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Abcb1 (GeneName), ATP-dependent translocase ABCB1 (ProteinName), MDR1_MOUSE.
Product Name:

Mouse Abcb1/ ATP-dependent translocase ABCB1 ELISA Kit

Cat.#:

E8039m

Brand:
EIAab®
Regulatory Status:
Alternative:

ATP-binding cassette sub-family B member 1B, P-glycoprotein 1, Abcb1b, Mdr1, Mdr1b, Pgy1, Pgy-1, Multidrug resistance protein 1B

Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
0.156-10ng/ml
Sensitivity:
0.078ng/mL
Specificity:
Natural and recombinant mouse ATP-dependent translocase ABCB1
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Assay Procedure
Research Area:
Signal Transduction
Mouse Abcb1 ELISA Kit
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Mouse Abcb1 ELISA Kit
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Product Datasheets
Datasheet: Down Datasheet
Instruction: Down Instruction
MSDS: MSDS


Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤6.5%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤9.4%

Recovery
Recovery was determined by spiking various levels of ATP-dependent translocase ABCB1 into serum and plasma.

Sample Type

Average(%)

Recovery Range(%)

Serum

89

83-95

Plasma

91

85-97

 

 

 

 

Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ATP-dependent translocase ABCB1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

80-93%

105-116%

89-97%

80-90%

EDTA plasma(n=5)

113-123%

100-110%

115-125%

111-120%

heparin plasma(n=5)

111-120%

 

101-111%

94-103%

91-103%

 

General Annotation


Sub Unit:
Interacts with PSMB5.


Function:
Energy-dependent efflux pump responsible for decreased drug accumulation in multidrug-resistant cells.


Subcellular Location:
Membrane Multi-pass membrane protein


This product has not yet been referenced specifically in any publications.

[1].
"Xenopus laevis oocytes expressing human P-glycoprotein: probing trans- and cis-inhibitory effects on [3H]vinblastine and [3H]digoxin efflux."

[2].
"Comparative description of haplotype structure and genetic diversity of MDR1 (ABCB1) in HIV-positive and HIV-negative populations."

[3].
"Breast cancer resistance protein and P-glycoprotein limit sorafenib brain accumulation."

[4].
"Influence of pharmacogenetics on response and toxicity in breast cancer patients treated with doxorubicin and cyclophosphamide."

[5].
"Genetic variations in ABCB1 and CYP3A5 as well as sex influence quinine disposition among Ugandans."
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