EIAab®ELISA Kits are in vitro enzyme-linked immunosorbent assays for the quantitative determination of concentrations of target antigen in a variety of species. Our kits provide researchers with a wide selection,including cytokines, growth factors, proteases, soluble receptors, apoptosis effectors, and many other small molecules. To ensure high quality and repeatability, ELISA Kits are thoroughly verified in house and undergo extremely stringent quality inspections. The scientific support team is on hand to provide you with any technical service about products.
Sandwich Test Principle
The Sandwich ELISA Kits are suitable for the detection and quantitative analysis of macromolecular antigens.
Competitive Test Principle
Target antigen in the sample competes with a fixed amount of biotin-antigen for sites on a pre-coated antibody.
Chemiluminescence immunoassay (CLIA) is a highly sensitive analytical technique for the quantitative detection of multiple antigens, haptens, antibodies, hormones, enzymes, fatty acids, vitamins and drugs by chemiluminescence assays combined with high specific immune responses. It is a novel immunoassay technique for enzyme analysis.
If you can select a make a specific antibody of plasma cells in culture, can be
formed by single cell by splitting proliferation of cells, namely monoclonal.
Polyclonal antibodiesA collection of immunoglobulins secreted by multiple plasma cells that are differentiated by stimulating multiple B cell clones to produce immune responses by a single antigen.
Monoclonal antibodiesA collection of immunoglobulins secreted by a single plasma cell that is differentiated by stimulating a single B cell clone to produce an immune response by a single antigenic epitope.
Recombinant Protein Recombinant proteins are obtained by the use of recombinant DNA or recombinant RNA. The expression platforms of recombinant protein mainly include five expression systems: escherichia coli, bacillus subtilis, yeast, mammalian cells and insect baculovirus cells.
That molecular weight is less than 500 molecules and small molecules are generally simple monomer substance.
Peptide is a biochemical substance between amino acid and protein. It is a protein fragment, smaller than the protein molecular weight and larger than the amino acid molecular weight.
Starting from the transcription product of a gene(such as mRNA), reverse transcription synthesizes complementary DNA(cDNA), which is then recombined into a carrier, After replication and screening to obtain cDNA molecules.
EIAab has a wide range of cell lysates from tissues and cell lines, including total protein lysates and cell lines lysates. The main source of tissue lysates are pathological tissue and normal tissue, and the cell lines lysates includes over-expression lysates of full length human proteins expressed in mammalian HEK293T cells as positive controls for antibody validation.
Tag/Control antibodies recognize some Target antigens such as His*6, GST, GAPDH,β-Actin etc.
Secondary antibodies recognize an antibody as their target antigen, usually referred to as the primary antibody which in turn is bound to its antigen or protein of interest.
Detection Reagent A
Assay Diluent A
Detection Reagent B
Assay Diluent B
Primary cells differ from immortalized cell lines by limited population doubling. Without genetic and chemical modifications, primary cells retain many of the important physiological properties of their origin tissue systems and closely mimic the in vivo conditions. They, therefore, offer ideal cell models for a vast variety of research, from fundamental cell biology and physiology, developmental biology, disease mechanisms, to drug screening and therapeutics development.
Recombinant proteins are obtained by the use of recombinant DNA or recombinant RNA. The expression platforms of recombinant protein mainly include five expression systems: escherichia coli, bacillus subtilis, yeast, mammalian cells and insect baculovirus cells.