Secondary Antibody

EIAab^®ELISA Kits are in vitro enzyme-linked immunosorbent assays for the quantitative determination of concentrations of target antigen in a variety of species. Our kits provide researchers with a wide selection,including cytokines, growth factors, proteases, soluble receptors, apoptosis effectors, and many other small molecules. To ensure high quality and repeatability, ELISA Kits are thoroughly verified in house and undergo extremely stringent quality inspections. The scientific support team is on hand to provide you with any technical service about products.

Sandwich Test Principle
The Sandwich ELISA Kits are suitable for the detection and quantitative analysis of macromolecular antigens.

Competitive Test Principle
Target antigen in the sample competes with a fixed amount of biotin-antigen for sites on a pre-coated antibody.

CLIA Kit

Chemiluminescence immunoassay (CLIA) is a highly sensitive analytical technique for the quantitative detection of multiple antigens, haptens, antibodies, hormones, enzymes, fatty acids, vitamins and drugs by chemiluminescence assays combined with high specific immune responses. It is a novel immunoassay technique for enzyme analysis.

Monoclonal antibody

If you can select a make a specific antibody of plasma cells in culture, can be formed by single cell by splitting proliferation of cells, namely monoclonal.

Polyclonal antibodies A collection of immunoglobulins secreted by multiple plasma cells that are differentiated by stimulating multiple B cell clones to produce immune responses by a single antigen.

Monoclonal antibodies A collection of immunoglobulins secreted by a single plasma cell that is differentiated by stimulating a single B cell clone to produce an immune response by a single antigenic epitope.

Recombinant Protein
Recombinant proteins are obtained by the use of recombinant DNA or recombinant RNA. The expression platforms of recombinant protein mainly include five expression systems: escherichia coli, bacillus subtilis, yeast, mammalian cells and insect baculovirus cells.

Small Molecule

That molecular weight is less than 500 molecules and small molecules are generally simple monomer substance.

Peptide

Peptide is a biochemical substance between amino acid and protein. It is a protein fragment, smaller than the protein molecular weight and larger than the amino acid molecular weight.

cDNA Clone

Starting from the transcription product of a gene(such as mRNA), reverse transcription synthesizes complementary DNA(cDNA), which is then recombined into a carrier, After replication and screening to obtain cDNA molecules.

Over-expression Lysate

EIAab has a wide range of cell lysates from tissues and cell lines, including total protein lysates and cell lines lysates. The main source of tissue lysates are pathological tissue and normal tissue, and the cell lines lysates includes over-expression lysates of full length human proteins expressed in mammalian HEK293T cells as positive controls for antibody validation.

Tag/Control Antibody

Epitope tag antibodies are highly specific antibodies for the detection and purification of the tagged proteins. EIAab offers a wide range of highly specific epitope tag antibodies to satisfy researchers' need, including His Tag antibodies, HA Tag antibodies, Myc Tag antibodies and GFP Tag antibodies, etc.
Loading control antibodies are against loading controls which are usually encoded by housekeeping genes and express stably at high levels in many cell lines and tissues. Some common loading control antibodies include, GAPDH、β -tubulin、β -actin etc.

Secondary Antibody

Secondary antibodies recognize an antibody as their target antigen, usually referred to as the primary antibody which in turn is bound to its antigen or protein of interest.

ELISA/ CLIA Kit Reagent

Cat.#	Reagent Name	Quantity/ Size
EPL+Cat.	Assay plate（ELISA）
CPL+Cat.	Assay plate（CLIA）
ST+Cat.	Standard
IC0001	Sample Diluent	*20mL
ED+Cat.	Detection Reagent A	*120μL
IC0002	Assay Diluent A	*10mL
EC+Cat.	Detection Reagent B	*120μL
IC0003	Assay Diluent B	*10mL
IC0006	Substrate	*10mL
IC0005	Stop Solution	*10mL
IC0007	Substrate A	*6mL
IC0008	Substrate B	*6mL
IC0004	Wash Buffer(25×)	*30mL

Cell Culture Consumable

Cat.#	Consumable Name	Quantity/ Size
iCell-02	24孔板专用细胞爬片	*100片
iCell-03	细胞培养皿	60mm/10个/包 100mm/10个/包
iCell-01	激光共聚焦培养皿	*10个/包
iCell-06	细胞培养瓶	T25/20个/包 T75/5个/包
iCell-09	方形血清瓶，培养基方瓶	*25个/包
iCell-010	巴氏吸管	*200支/盒
iCell-011	离心管	15ml/50个/包 50ml/25个/包
iCell-012	1.8ml细胞冻存管\|冷冻管	*50个/包
iCell-013	PCR薄壁反应管	*0.2ml
iCell-014	微量离心管	*500支/包
iCell-015	细胞刮、铲刀	*1支
66007-1-Ig	V5-Tag Antibody	*100 μl
iCell-15140-122	青霉素-链霉素	*100ml
iCell-17100-017	胶原酶I	*100mg
iCell-17101-015	胶原酶II	*100mg
iCell-17104-019	胶原酶IV	*100mg
iCell-016	一次性无菌移液管	5ml/50支/包 10ml/50支/包 *25ml/50支/包
iCell-017	细胞培养孔板	*6/12/24/48/96

Primary Cells

Primary cells differ from immortalized cell lines by limited population doubling. Without genetic and chemical modifications, primary cells retain many of the important physiological properties of their origin tissue systems and closely mimic the in vivo conditions. They, therefore, offer ideal cell models for a vast variety of research, from fundamental cell biology and physiology, developmental biology, disease mechanisms, to drug screening and therapeutics development.

Cell Line

A cell line refers to a population of cells that have been propagated after the primary cell culture has been successfully passaged for the first time. It also refers to cultured cells that can be passaged for a long period of time. (This led to the subsequent FiniteCellLine and InfiniteCellLine). Therefore, a cell line narrowly refers to a cell that can be passaged continuously (used in both spoken and written languages in a specific environment). Refers to cells that can be passaged.

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Recombinant Protein

Recombinant proteins are obtained by the use of recombinant DNA or recombinant RNA. The expression platforms of recombinant protein mainly include five expression systems: escherichia coli, bacillus subtilis, yeast, mammalian cells and insect baculovirus cells.