Secondary Antibody

EIAab^®ELISA Kits are in vitro enzyme-linked immunosorbent assays for the quantitative determination of concentrations of target antigen in a variety of species. Our kits provide researchers with a wide selection,including cytokines, growth factors, proteases, soluble receptors, apoptosis effectors, and many other small molecules. To ensure high quality and repeatability, ELISA Kits are thoroughly verified in house and undergo extremely stringent quality inspections. The scientific support team is on hand to provide you with any technical service about products.

Sandwich Test Principle
The Sandwich ELISA Kits are suitable for the detection and quantitative analysis of macromolecular antigens.

Competitive Test Principle
Target antigen in the sample competes with a fixed amount of biotin-antigen for sites on a pre-coated antibody.

CLIA Kit

Chemiluminescence immunoassay (CLIA) is a highly sensitive analytical technique for the quantitative detection of multiple antigens, haptens, antibodies, hormones, enzymes, fatty acids, vitamins and drugs by chemiluminescence assays combined with high specific immune responses. It is a novel immunoassay technique for enzyme analysis.

Monoclonal antibody

If you can select a make a specific antibody of plasma cells in culture, can be formed by single cell by splitting proliferation of cells, namely monoclonal.

Polyclonal antibodies A collection of immunoglobulins secreted by multiple plasma cells that are differentiated by stimulating multiple B cell clones to produce immune responses by a single antigen.

Monoclonal antibodies A collection of immunoglobulins secreted by a single plasma cell that is differentiated by stimulating a single B cell clone to produce an immune response by a single antigenic epitope.

Recombinant Protein
Recombinant proteins are obtained by the use of recombinant DNA or recombinant RNA. The expression platforms of recombinant protein mainly include five expression systems: escherichia coli, bacillus subtilis, yeast, mammalian cells and insect baculovirus cells.

Peptide

Peptide is a biochemical substance between amino acid and protein. It is a protein fragment, smaller than the protein molecular weight and larger than the amino acid molecular weight.

cDNA Clone

Starting from the transcription product of a gene(such as mRNA), reverse transcription synthesizes complementary DNA(cDNA), which is then recombined into a carrier, After replication and screening to obtain cDNA molecules.

Over-expression Lysate

EIAab has a wide range of cell lysates from tissues and cell lines, including total protein lysates and cell lines lysates. The main source of tissue lysates are pathological tissue and normal tissue, and the cell lines lysates includes over-expression lysates of full length human proteins expressed in mammalian HEK293T cells as positive controls for antibody validation.

Secondary Antibody

Secondary antibodies recognize an antibody as their target antigen, usually referred to as the primary antibody which in turn is bound to its antigen or protein of interest.

Kit Reagent

Cat.#	Reagent Name	Quantity
EPL+Cat.	Assay plate（ELISA）
CPL+Cat.	Assay plate（CLIA）
ST+Cat.	Standard
IC0001	Sample Diluent	*20mL
ED+Cat.	Detection Reagent A	*120μL
IC0002	Assay Diluent A	*10mL
EC+Cat.	Detection Reagent B	*120μL
IC0003	Assay Diluent B	*10mL
IC0006	Substrate	*10mL
IC0005	Stop Solution	*10mL
IC0007	Substrate A	*6mL

Primary Cells

Primary cells differ from immortalized cell lines by limited population doubling. Without genetic and chemical modifications, primary cells retain many of the important physiological properties of their origin tissue systems and closely mimic the in vivo conditions. They, therefore, offer ideal cell models for a vast variety of research, from fundamental cell biology and physiology, developmental biology, disease mechanisms, to drug screening and therapeutics development.

Recombinant Protein

Recombinant proteins are obtained by the use of recombinant DNA or recombinant RNA. The expression platforms of recombinant protein mainly include five expression systems: escherichia coli, bacillus subtilis, yeast, mammalian cells and insect baculovirus cells.