027-59234612(+86)
EIAab
Home  >  ELISA Kit  >  Human ABCB1 ELISA Kit
ABCB1 (GeneName), ATP-dependent translocase ABCB1 (ProteinName), MDR1_HUMAN.
Product Name:

Human ABCB1/ ATP-dependent translocase ABCB1 ELISA Kit

Cat.#:

E8039h

Brand:
EIAab®
Regulatory Status:
Alternative:

ATP-binding cassette sub-family B member 1, MDR1, PGY1, Multidrug resistance protein 1, P-glycoprotein 1, Phospholipid transporter ABCB1, CD243

Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
0.156-10ng/mL
Sensitivity:
0.076ng/mL
Specificity:
Natural and recombinant human ATP-dependent translocase ABCB1
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Assay Procedure
Research Area:
Signal Transduction
Human ABCB1 ELISA Kit
Size & Price: Sign In.
×
Human ABCB1 ELISA Kit
Email *
Message *
Please Sign In.
Product Datasheets
Datasheet: Down Datasheet
Instruction: Down Instruction
MSDS: MSDS


Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤5.3%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤9.2%

Recovery
Recovery was determined by spiking various levels of ATP-dependent translocase ABCB1 into serum and plasma.

Sample Type

Average(%)

Recovery Range(%)

Serum

86

80-92

Plasma

88

82-94

 

 

 

 

Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ATP-dependent translocase ABCB1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

87-98%

95-105%

110-120%

92-102%

EDTA plasma(n=5)

109-120%

105-115%

104-114%

101-109%

heparin plasma(n=5)

94-105%

 

106-115%

98-107%

107-117%

 

General Annotation


Sub Unit:
Interacts with PSMB5. Finds in a complex with ABCB1, TFPI2 and PPP2R3C; leading to the dephosphorylation of ABCB1.


Function:
Translocates drugs and phospholipids across the membrane (PubMed:8898203, PubMed:2897240, PubMed:9038218). Catalyzes the flop of phospholipids from the cytoplasmic to the exoplasmic leaflet of the apical membrane. Participates mainly to the flop of phosphatidylcholine, phosphatidylethanolamine, beta-D-glucosylceramides and sphingomyelins (PubMed:8898203). Energy-dependent efflux pump responsible for decreased drug accumulation in multidrug-resistant cells (PubMed:2897240, PubMed:9038218).


Subcellular Location:
Cell membrane Multi-pass membrane protein Apical cell membrane


This product has not yet been referenced specifically in any publications.

[1].
"Xenopus laevis oocytes expressing human P-glycoprotein: probing trans- and cis-inhibitory effects on [3H]vinblastine and [3H]digoxin efflux."

[2].
"Comparative description of haplotype structure and genetic diversity of MDR1 (ABCB1) in HIV-positive and HIV-negative populations."

[3].
"Breast cancer resistance protein and P-glycoprotein limit sorafenib brain accumulation."

[4].
"Influence of pharmacogenetics on response and toxicity in breast cancer patients treated with doxorubicin and cyclophosphamide."

[5].
"Genetic variations in ABCB1 and CYP3A5 as well as sex influence quinine disposition among Ugandans."
Close
Sample Data
Close
Sample Data
Close
Sample Data
My Cart close
Attention close
History close
Clear All
Comparison close
User
My Cart
Attention
History
Comparison
Top
Message
close_message
Product Name *
Email *
Message: *
Inform
new Message
Size Quantity Price ($) Subtotal 1 ($)
Subtotal 2: $
triangle
Size Quantity Price ($)
Do you want to be our agent and get a lower discount?
Please contact us:
Tel:027-59234612(+86)
Fax:027-59759960(+86)
Email:sales@eiaab.com