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ABCA7 (GeneName), Phospholipid-transporting ATPase ABCA7 (ProteinName), ABCA7_HUMAN.
Product Name:

Human ABCA7/ Phospholipid-transporting ATPase ABCA7 ELISA Kit

Cat.#:

E15004h

Brand:
EIAab®
Regulatory Status:
Alternative:

ABCA-SSN, ATP-binding cassette sub-family A member 7, Autoantigen SS-N, Macrophage ABC transporter

Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
0.156-10ng/ml
Sensitivity:
0.045ng/ml
Specificity:
Natural and recombinant human Phospholipid-transporting ATPase ABCA7
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Assay Procedure
Research Area:
Cell Biology
Human ABCA7 ELISA Kit
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Human ABCA7 ELISA Kit
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Product Datasheets
Datasheet: Down Datasheet
Instruction: Down Instruction
MSDS: MSDS


Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤3.2%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.4%

Recovery
Recovery was determined by spiking various levels of Phospholipid-transporting ATPase ABCA7 into serum and plasma.

Sample Type

Average(%)

Recovery Range(%)

Serum

100

94-106

Plasma

102

96-108

 

 

 

 

Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Phospholipid-transporting ATPase ABCA7 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

100-100%

109-119%

84-92%

83-93%

EDTA plasma(n=5)

106-116%

109-119%

101-110%

104-114%

heparin plasma(n=5)

82-95%

 

108-118%

85-95%

116-126%

 

General Annotation


Sub Unit:
N/A


Function:
Catalyzes the translocation of specific phospholipids from the cytoplasmic to the extracellular/lumenal leaflet of membrane coupled to the hydrolysis of ATP (PubMed:24097981). Transports preferentially phosphatidylserine over phosphatidylcholine (PubMed:24097981). Plays a role in lipid homeostasis and macrophage-mediated phagocytosis (PubMed:14592415, PubMed:12917409, PubMed:12925201, PubMed:14570867). Binds APOA1 and may function in apolipoprotein-mediated phospholipid efflux from cells (PubMed:12917409, PubMed:14570867, PubMed:14592415). May also mediate cholesterol efflux (PubMed:14570867). May regulate cellular ceramide homeostasis during keratinocyte differentiation (PubMed:12925201). Involved in lipid raft organization and CD1D localization on thymocytes and antigen-presenting cells, which plays an important role in natural killer T-cell development and activation (By similarity). Plays a role in phagocytosis of apoptotic cells by macrophages (By similarity). Macrophage phagocytosis is stimulated by APOA1 or APOA2, probably by stabilization of ABCA7 (By similarity). Also involved in phagocytic clearance of amyloid-beta by microglia cells and macrophages (By similarity). Further limits amyloid-beta production by playing a role in the regulation of amyloid-beta A4 precursor protein (APP) endocytosis and/or processing (PubMed:26260791). Amyloid-beta is the main component of amyloid plaques found in the brains of Alzheimer patients (PubMed:26260791).


Subcellular Location:
Isoform 2 Cytoplasm Endoplasmic reticulum May localize to the endoplasmic reticulum.


This product has not yet been referenced specifically in any publications.

[1].
"Human ABCA7 supports apolipoprotein-mediated release of cellular cholesterol and phospholipid to generate high density lipoprotein."

[2].
"Identification of a novel human sterol-sensitive ATP-binding cassette transporter (ABCA7)."

[3].
"Loss-of-function variants in ABCA7 confer risk of Alzheimer's disease."

[4].
"Mutations in ABCA7 in a Belgian cohort of Alzheimer's disease patients: a targeted resequencing study."

[5].
"Common variants at ABCA7, MS4A6A/MS4A4E, EPHA1, CD33 and CD2AP are associated with Alzheimer's disease."

[6].
"Common variants at MS4A4/MS4A6E, CD2AP, CD33 and EPHA1 are associated with late-onset Alzheimer's disease."

[7].
"Genetic susceptibility to distinct bladder cancer subphenotypes."

[8].
"Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study."

[9].
"Common genetic variation in multiple metabolic pathways influences susceptibility to low HDL-cholesterol and coronary heart disease."

[10].
"Polymorphisms in innate immunity genes and lung cancer risk in Xuanwei, China."
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