Pig Proinsulin ELISA Kit

Cat.#:

E0379p

Brand:

EIAab^®

Regulatory Status:

RUO

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

0.625-40pmol/ml

Sensitivity:

0.26pmol/ml

Specificity:

Natural and recombinant pig Proinsulin

Sample Type:

Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids

Sample Data:

Assay Procedure:

Research Area:

Signal Transduction

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Pig Proinsulin ELISA Kit

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Product Datasheets

Datasheet:

Instruction:

MSDS:

Data
Citations
Sequence / 3D
Feedback Wall (0)

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Proinsulin and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	103-111%	106-116%	105-113%	112-120%
EDTA plasma(n=5)	87-97%	86-96%	88-96%	96-106%
heparin plasma(n=5)	102-114%	82-91%	80-90%	101-111%

General Annotation

Sub Unit:

N/A

Function:

Proinsulin is the prohormone precursor to insulin made in the beta cells of the islets of Langerhans, specialized regions of the pancreas. In humans, proinsulin is encoded by the INS gene.

Location:

Proinsulin is synthesized in the endoplasmic reticulum, where it is folded and its disulfide bonds are oxidized. It is then transported to the Golgi apparatus where it is packaged into secretory vesicles, and where it is processed by a series of proteases to form mature insulin. Mature insulin has 35 fewer amino acids; 4 are removed altogether, and the remaining 31 form the C-peptide. The C-peptide is abstracted from the center of the proinsulin sequence; the two other ends (the B chain and A chain) remain connected by disulfide bonds.

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