General ET/ Endotoxin ELISA Kit

Cat.#:

E0818Ge

Brand:

EIAab^®

Regulatory Status:

RUO

Detection Method:

ELISA

Assay Type:

Competitive

Detection Range:

0.0156-1EU/mL

Sensitivity:

0.005EU/mL

Specificity:

Natural and recombinant general Endotoxin

Sample Type:

Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids

Sample Data:

Assay Procedure:

Research Area:

Product Overview:

E0818Ge is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A Disintegrin and Metalloprotease 30 (Endotoxin) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Endotoxin. The ELISA analytical biochemical technique of the E0818Ge kit is based on Endotoxin antibody-Endotoxin antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Endotoxin antigen targets in samples. The ELISA Kit is designed to detect native, recombinant, Endotoxin. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

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General ET ELISA Kit

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Product Datasheets

Datasheet:

Instruction:

MSDS:

Data
Citations ( 2 )
Sequence / 3D
Feedback Wall (0)

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Endotoxin and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	105-114%	95-105%	92-101%	107-117%
EDTA plasma(n=5)	89-101%	80-80%	106-118%	108-118%
heparin plasma(n=5)	111-120%	95-104%	101-113%	104-113%

General Annotation

Sub Unit:

N/A

Function:

Endotoxins are a component of the cell wall of certain bacteria. Bacteria which contain endotoxin are known as gram negative bacteria. The endotoxins are released from the bacteria as they are destroyed and elicit a strong response from the immune system by activating various components of the immune system and inducing cytokine release.

Location:

N/A

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