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Calcitriol (Shorten Name),
Product Name:

General Calcitriol ELISA Kit

 
Cat.#:

E0467Ge

Brand:
EIAab®
Regulatory Status:
Alternative:

1,25-dihydroxycholecalciferol,1,25-dihydroxyvitamin D3

Detection Method:
ELISA
Assay Type:
Competitive
Detection Range:
15.6-1000pg/mL
Sensitivity:
3.6pg/mL
Specificity:
Natural and recombinant general Calcitriol
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Research Area:
-
Product Overview:
E0467Ge is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A Disintegrin and Metalloprotease 30 (Calcitriol) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Calcitriol. The ELISA analytical biochemical technique of the E0467Ge kit is based on Calcitriol antibody-Calcitriol antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Calcitriol antigen targets in samples. The ELISA Kit is designed to detect native, recombinant, Calcitriol. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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General Calcitriol ELISA Kit
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Product Datasheets
Datasheet:
Instruction:
MSDS:


Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: 4.7


Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: 8.6%

Recovery
Recovery was determined by spiking various levels of Calcitriol into serum and plasma.

Recovery was determined by spiking various levels of Calcitriol into serum and plasma .

Sample Type

Average(%)

Recovery  Range (%)

Serum

90

88-98

Plasma

84

79-93

Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Calcitriol and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

110-119%

100-110%

100-110%

108-117%

EDTA plasma(n=5)

114-123%

91-101%

85-97%

107-117%

heparin plasma(n=5)

107-116%

 

105-115%

100-110%

103-113%

 

General Annotation


Sub Unit:
N/A


Function:
Calcitriol, also called 1,25-dihydroxycholecalciferol or 1,25-dihydroxyvitamin D3, is the hormonally active form of vitamin D with three hydroxyl groups (abbreviated 1,25-(OH)2D3 or simply 1,25(OH)2D). It increases the level of calcium (Ca2+) in the blood by (1) increasing the uptake of calcium from the gut into the blood, (2) decreasing the transfer of calcium from blood to the urine by the kidney, and (3) increasing the release of calcium into the blood from bone.


Location:
Calcitriol is produced in the cells of the proximal tubule of the nephron in the kidneys by the action of 25-hydroxyvitamin D3 1-alpha-hydroxylase, a mitochondrial oxygenase and an enzyme which catalyzes the hydroxylation of 25-hydroxycholecalciferol (calcifediol). The activity of the enzyme is stimulated by PTH. The reaction is an important control point in Ca2+ homeostasis. The production of calcitriol is also increased by prolactin, a hormone which stimulates lactogenesis (the formation of breast milk), a process which requires large amounts of calcium. It is decreased by high levels of serum phosphate and by an increase in the production of the hormone FGF-23 by osteocyte cells in bone.


Sample Data
Sample Data
Sample Data
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