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Calcitriol (短名 ),
产品名称:

General Calcitriol ELISA Kit
1,25二羟基维生素D3/骨化三醇

货号:

E0467Ge

商标:
EIAab®
监管等级:
别名:

1, 25-dihydroxycholecalciferol, 1, 25-dihydroxyvitamin D3

检测方法:
ELISA
实验类型:
Competitive
检测范围:
15.6-1000pg/mL
灵敏度:
3.6pg/mL
特异性:
Natural and recombinant general Calcitriol
样品类型:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
样品数据:
实验步骤:
实验步骤
研究领域:
-
General Calcitriol ELISA Kit
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General Calcitriol ELISA Kit
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产品说明书
数据表: 下载说明书
说明书: 下载说明书
MSDS: MSDS


精密度
批内差:已知浓度的3个样本在一个板子内重复检测20次,以评估批内精密度。
批内 CV: 4.7%
批间差:已知浓度的3个样本在不同的板子上重复测定5次,以评估测定批间精密度。
批间 CV: 8.6%
回收率
回收率:低、中和高浓度的分析物被掺入到血清或者血浆样本中,进行回收实验测定。

Recovery was determined by spiking various levels of Calcitriol into serum and plasma .

Sample Type

Average(%)

Recovery  Range (%)

Serum

90

88-98

Plasma

84

79-93

线性
线性:给定样本通过梯度稀释,每次稀释的测量值与理论值的比值。

Sample

1:2

1:4

1:8

1:16

serum(n=5)

110-119%

100-110%

100-110%

108-117%

EDTA plasma(n=5)

114-123%

91-101%

85-97%

107-117%

heparin plasma(n=5)

107-116%

 

105-115%

100-110%

103-113%

 

通用注释


亚单元:
N/A


功能:
Calcitriol, also called 1,25-dihydroxycholecalciferol or 1,25-dihydroxyvitamin D3, is the hormonally active form of vitamin D with three hydroxyl groups (abbreviated 1,25-(OH)2D3 or simply 1,25(OH)2D). It increases the level of calcium (Ca2+) in the blood by (1) increasing the uptake of calcium from the gut into the blood, (2) decreasing the transfer of calcium from blood to the urine by the kidney, and (3) increasing the release of calcium into the blood from bone.


Location:
Calcitriol is produced in the cells of the proximal tubule of the nephron in the kidneys by the action of 25-hydroxyvitamin D3 1-alpha-hydroxylase, a mitochondrial oxygenase and an enzyme which catalyzes the hydroxylation of 25-hydroxycholecalciferol (calcifediol). The activity of the enzyme is stimulated by PTH. The reaction is an important control point in Ca2+ homeostasis. The production of calcitriol is also increased by prolactin, a hormone which stimulates lactogenesis (the formation of breast milk), a process which requires large amounts of calcium. It is decreased by high levels of serum phosphate and by an increase in the production of the hormone FGF-23 by osteocyte cells in bone.


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