首页 > 重组蛋白 > Mouse Csrp3 Recombinant Protein

Csrp3 (基因名), Cysteine and glycine-rich protein 3 (蛋白名), csrp3_mouse.

产品名称:

Mouse Csrp3/ Cysteine and glycine-rich protein 3 Recombinant Protein
富含半胱氨酸和甘氨酸的蛋白质

货号:

R15404m

商标:

EIAab^®

监管等级:

RUO

别名:

Cysteine-rich protein 3, LIM domain protein, cardiac, Muscle LIM protein, CRP3, Clp, Mlp

序列号:

P50462

来源:

E.coli

种属:

Mouse

标签:

His

纯度:

>90% by SDS-PAGE

浓度:

Reconstitution Dependent

形态:

Liquid

内毒素水平:

Please contact protein@eiaab.com The technician for more information.

应用:

存储缓冲液:

50mM NaH2PO4, 500mM NaCl Buffer with 500mM Imidazole, 10%glycerol(PH8.0)

存储:

Store at -20°C. (Avoid repeated freezing and thawing.)

研究领域:

规格 & 价格:

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Mouse Csrp3 Protein

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产品说明书

说明书:

MSDS:

在线询价

数据
文献
序列 / 3D
常见问题解答
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R&D 技术数据

更多信息，请参阅手册，或联系我们的技术支持: tech@eiaab.com.

通用注释

亚单元:

Self-associates. Oligomeric in the cytoplasm and monomeric in the nucleus. Homooligomers preferentially form along the actin cytoskeleton (By similarity). Interacts with TCAP (PubMed:20044516). Interacts with LDHD, MYOD1, MYOG, ACTN2, NRAP, MYF6 (By similarity). Interacts (via N-terminus)D with GLRX3 (via C-terminus) and PPP3CA; GLRX3 and calcineurin compete for interaction with CSRP3 (PubMed:18258855). Interacts with CFL2; the stoichiometry influences F-actin depolymerization and possibly two molecules of CFL2 can interact with one molecule of CSRP3 resulting in the highest functional impact; the interaction is stronger with phosphorylated CFL2.

功能:

Positive regulator of myogenesis. Acts as cofactor for myogenic bHLH transcription factors such as MYOD1, and probably MYOG and MYF6. Enhances the DNA-binding activity of the MYOD1:TCF3 isoform E47 complex and may promote formation of a functional MYOD1:TCF3 isoform E47:MEF2A complex involved in myogenesis (By similarity). Plays a crucial and specific role in the organization of cytosolic structures in cardiomyocytes. Could play a role in mechanical stretch sensing. May be a scaffold protein that promotes the assembly of interacting proteins at Z-line structures. It is essential for calcineurin anchorage to the Z line. Required for stress-induced calcineurin-NFAT activation (PubMed:9039266, PubMed:15665106). The role in regulation of cytoskeleton dynamics by association with CFL2 is reported conflictingly. Proposed to contribute to the maintenance of muscle cell integerity through an actin-based mechanism. Can directly bind to actin filaments, cross-link actin filaments into bundles without polarity selectivity and protect them from dilution- and cofilin-mediated depolymerization; the function seems to involve its self-association (By similarity). In vitro can inhibit PKC/PRKCA activity. Proposed to be involved in cardiac stress signaling by down-regulating excessive PKC/PRKCA signaling (PubMed:27353086).

亚细胞位置:

Nucleus Cytoplasm Cytoplasm Cytoskeleton Cytoplasm Myofibril Sarcomere Z line Cytoplasm Myofibril Sarcomere Nucleocytoplasmic shuttling protein (By similarity). Mainly cytoplasmic. In the Z line, found associated with GLRX3 (via C-terminus).

数据库链接

UniGene:

Mm.17235

SMR:

P50462

STRING:

10090.ENSMUSP00000032658

KEGG:

mmu:13009

Pfam:

PF00412

Uniprot:

P50462

相关产品

Human CSRP3 ELISA Kit

Rat Csrp3 ELISA Kit

Bovine CSRP3 ELISA Kit

Human CSRP3 CLIA Kit

Rat Csrp3 CLIA Kit

序列长度:

194

序列:

仅供参考，详细请咨询（services@eiaab.com）

50:

100:

150:

194:

GKSLESTNVT | DKDGELYCKV | CYAKNFGPTG | IGFGGLTQQV | EKKE

3D 模型:

3D 结构:

免疫原序列:

验证序列:

该产品尚未在任何出版物中被引用。

[1].

小鼠富含半胱氨酸和甘氨酸的蛋白质(Csrp3)重组蛋白是否是无菌的？

蛋白试剂瓶和蛋白保存液是经过高压灭菌的，但也不能保证蛋白是完全无菌的。如果要求蛋白是无菌的，可以用0.2微米的滤器对蛋白进行过滤。

[2].

小鼠富含半胱氨酸和甘氨酸的蛋白质(Csrp3)重组蛋白的保存缓冲液是什么？

纯化后的蛋白保存在PBS（58mM Na2HPO4, 17mM NaH2PO4, 68mM NaCl, pH7.4）里，并往里面加入500mM咪唑和10%甘油。

[3].

怎样确定小鼠富含半胱氨酸和甘氨酸的蛋白质(Csrp3)重组蛋白的浓度？

蛋白浓度的确定没有一个统一的标准，这主要取决于蛋白的氨基酸序列。伊艾博是根据不同测试的组合来测定蛋白浓度。考马斯亮蓝法、BCA法、氨基酸序列和氨基酸全序列分析法等都用来测定蛋白浓度。

[4].

小鼠富含半胱氨酸和甘氨酸的蛋白质(Csrp3)重组蛋白蛋白保存条件是怎样的？

蛋白应保存在 -20℃或 -80℃条件下，为了避免反复冻融，可以将蛋白分装成小份保存。

[5].

小鼠富含半胱氨酸和甘氨酸的蛋白质(Csrp3)重组蛋白是否可以用于活体实验？

重组蛋白没有用于任何的活体实验，因此蛋白的活性和半衰期是不确定的。

[6].

小鼠富含半胱氨酸和甘氨酸的蛋白质(Csrp3)重组蛋白的保质期是多久？

在适当的保存条件下，从购买之日起蛋白可以稳定保存6-12个月。适当的保存条件是:蛋白保存在-20°C o或 -80℃，保证蛋白的保存浓度高于0.1mg/ml，限制蛋白反复冻融的次数。我们公司常规的质量检测保证所有产品在销售时都有可接受的生物活性。但是我们不能控制终端用户蛋白的保存条件。如果产品在有效期内出现问题，请联系我们的技术支持。

[7].

你们蛋白和抗体的报价是怎么样的？

我们将根据你需要的蛋白和抗体的大小进行报价。

[8].

小鼠富含半胱氨酸和甘氨酸的蛋白质(Csrp3)重组蛋白是否能够提供蛋白片段？

我们现有的人的蛋白的序列可以有很多。你可以选择你感兴趣的靶向部分，我们将会按您的需求提供蛋白和抗体。

[9].

小鼠富含半胱氨酸和甘氨酸的蛋白质(Csrp3)重组蛋白的货期或发货时间一般是多长？

具体指标的货期需要确定。最快一周，最长可能一个月。

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