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Tigar (基因名), Fructose-2,6-bisphosphatase TIGAR (蛋白名), tigar_mouse.

产品名称:

Mouse Tigar/ Fructose-2,6-bisphosphatase TIGAR ELISA Kit
果糖-2,6-二磷酸酶TIGAR

货号:

E5948m

商标:

EIAab^®

监管等级:

RUO

别名:

TP53-induced glycolysis and apoptosis regulator, TP53-induced glycolysis regulatory phosphatase

检测方法:

ELISA

实验类型:

Sandwich

检测范围:

0.78-50ng/mL

灵敏度:

0.392ng/mL

特异性:

Natural and recombinant mouse Fructose-2,6-bisphosphatase TIGAR

样品类型:

Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids

样品数据:

实验步骤:

研究领域:

规格 & 价格:

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Mouse Tigar ELISA Kit

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产品说明书

数据表:

说明书:

MSDS:

在线询价

数据
文献
序列 / 3D
常见问题解答
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精密度

批内差：已知浓度的3个样本在一个板子内重复检测20次，以评估批内精密度。
批内 CV： ≤5.8%
批间差：已知浓度的3个样本在不同的板子上重复测定5次，以评估测定批间精密度。
批间 CV： ≤8.3%

回收率

回收率：低、中和高浓度的分析物被掺入到血清或者血浆样本中，进行回收实验测定。

Sample Type	Average(%)	Recovery Range(%)
Serum	111	105-117
Plasma	113	107-119

线性

线性：给定样本通过梯度稀释，每次稀释的测量值与理论值的比值。

Sample	1：2	1:4	1:8	1:16
serum(n=5)	103-115%	107-116%	103-114%	100-112%
EDTA plasma(n=5)	103-114%	114-124%	106-115%	91-101%
heparin plasma(n=5)	97-107%	101-110%	104-114%	89-101%

通用注释

亚单元:

Interacts with HK2; the interaction increases hexokinase HK2 activity in a hypoxia- and HIF1A-dependent manner, resulting in the regulation of mitochondrial membrane potential, thus increasing NADPH production and decreasing intracellular ROS levels.

功能:

Fructose-bisphosphatase hydrolyzing fructose-2,6-bisphosphate as well as fructose-1,6-bisphosphate (By similarity). Acts as a negative regulator of glycolysis by lowering intracellular levels of fructose-2,6-bisphosphate in a p53/TP53-dependent manner, resulting in the pentose phosphate pathway (PPP) activation and NADPH production (PubMed:23726973). Contributes to the generation of reduced glutathione to cause a decrease in intracellular reactive oxygen species (ROS) content, correlating with its ability to protect cells from oxidative or metabolic stress-induced cell death (PubMed:23726973). Plays a role in promoting protection against cell death during hypoxia by decreasing mitochondria ROS levels in a HK2-dependent manner through a mechanism that is independent of its fructose-bisphosphatase activity (By similarity). In response to cardiac damage stress, mediates p53-induced inhibition of myocyte mitophagy through ROS levels reduction and the subsequent inactivation of BNIP3 (PubMed:22044588). Reduced mitophagy results in an enhanced apoptotic myocyte cell death, and exacerbates cardiac damage (PubMed:22044588). Plays a role in adult intestinal regeneration; contributes to the growth, proliferation and survival of intestinal crypts following tissue ablation (PubMed:23726973). Plays a neuroprotective role against ischemic brain damage by enhancing PPP flux and preserving mitochondria functions (PubMed:24872551). Protects glioma cells from hypoxia- and ROS-induced cell death by inhibiting glycolysis and activating mitochondrial energy metabolism and oxygen consumption in a TKTL1-dependent and p53/TP53-independent manner. Plays a role in cancer cell survival by promoting DNA repair through activating PPP flux in a CDK5-ATM-dependent signaling pathway during hypoxia and/or genome stress-induced DNA damage responses (By similarity). Involved in intestinal tumor progression (PubMed:23726973).

亚细胞位置:

Cytoplasm Nucleus Mitochondrion Translocated to the mitochondria during hypoxia in a HIF1A-dependent manner. Colocalizes with HK2 in the mitochondria during hypoxia. Translocated to the nucleus during hypoxia and/or genome stress-induced DNA damage responses in cancer cells (By similarity). Translocation to the mitochondria is enhanced in ischemic cortex after reperfusion and/or during oxygen and glucose deprivation (OGD)/reoxygenation insult in primary neurons (PubMed:24872551).

数据库链接

UniGene:

Mm.101836

SMR:

Q8BZA9

STRING:

10090.ENSMUSP00000048643

KEGG:

mmu:319801

Pfam:

PF00300

Uniprot:

Q8BZA9

相关产品

Human TIGAR ELISA Kit

Bovine TIGAR ELISA Kit

Human TIGAR CLIA Kit

Bovine TIGAR CLIA Kit

Human TIGAR Protein

序列长度:

269

序列:

仅供参考，详细请咨询（services@eiaab.com）

50:

100:

150:

200:

250:

269:

QCVCMNLQEH | LNGVTEKQH

3D 模型:

3D 结构:

免疫原序列:

验证序列:

该产品尚未在任何出版物中被引用。

[1].

小鼠果糖-2,6-二磷酸酶TIGAR(Tigar)ELISA试剂盒可以做多少个样本？

小鼠果糖-2,6-二磷酸酶TIGAR(Tigar)ELISA试剂盒分为2种规格，96孔和48孔。96孔的试剂盒，标曲和样本都做复孔的话，可以检测40个样本。96孔的试剂盒，标曲和样本都不做复孔的话，可以检测88个样本。

[2].

小鼠果糖-2,6-二磷酸酶TIGAR(Tigar)ELISA试剂盒使用视频？

小鼠果糖-2,6-二磷酸酶TIGAR(Tigar)ELISA试剂盒实验操作视频在以下网址中，对每一步的实验步骤都做了演示，方便实验员能更好地理解ELISA实验的过程。
https://www.eiaab.com.cn/lesson-tech/805.html

[3].

小鼠果糖-2,6-二磷酸酶TIGAR(Tigar)ELISA试剂盒是放在-20℃冰箱保存吗？

EIAab的小鼠果糖-2,6-二磷酸酶TIGAR(Tigar)ELISA试剂盒，洗涤液、底物、终止液保存于4℃，其余试剂-20℃冰箱保存。

[4].

小鼠果糖-2,6-二磷酸酶TIGAR(Tigar)ELISA试剂盒原理？

双抗体夹心法：用纯化的抗体包被微孔板，制成固相抗体，往包被有固相抗体的微孔中依次加入标准品或受检样本、生物素化抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。用酶标仪在450nm波长下测定吸光度(OD值）,计算样本浓度。
竞争法：用纯化的抗体包被微孔板，制成固相抗体，往包被有固相抗体的微孔中依次加入标准品或受检样本和生物素标记的目标分析物，受检标本中抗原与生物素标记抗原竞争结合有限的抗体。再加入HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。用酶标仪在450nm波长下测定吸光度(OD值）,计算样本浓度。

[5].

小鼠果糖-2,6-二磷酸酶TIGAR(Tigar)ELISA试剂盒中需要使用的样品量是多少？

夹心法100μL/孔，竞争法50μL/孔。如样本浓度过高时，应对样本进行稀释，以使稀释后的样本符合试剂盒的检测范围，计算时再乘以相应的稀释倍数。

[6].

如何分析小鼠果糖-2,6-二磷酸酶TIGAR(Tigar)ELISA试剂盒数据？

建议标准曲线，并计算样本浓度。对于elisa的曲线拟合，一般建议采用4参数曲线拟合，4参数曲线拟合通常更适合免疫分析。推荐使用专业软件进行曲线拟合，例如curve expert 1.3。根据样本的OD值由标曲查出相应的浓度，再乘以稀释倍数；或用标准物的浓度与OD值计算出标曲的回归方程式，将样本的OD值代入方程式，计算出样本浓度，再乘以稀释倍数，即为样本的实际浓度。以下链接是curve expert 1.3软件拟合曲线的方法。
https://www.eiaab.com.cn/news/502/

[7].

小鼠果糖-2,6-二磷酸酶TIGAR(Tigar)ELISA试剂盒中是否包含人和动物的副产物，是否包含感染的或者传染性原料如HIV等？

除了抗体和稀释液中的BSA，不含其它人和动物的副产物，也不含感染材料。

[8].

收集小鼠果糖-2,6-二磷酸酶TIGAR(Tigar)ELISA试剂盒血浆样本，用什么作为抗凝剂？

一般建议用EDTA和肝素作为抗凝剂。

[9].

小鼠果糖-2,6-二磷酸酶TIGAR(Tigar)ELISA试剂盒酶标板可以拆成几部分？拆的时候是否需要避光，无菌？

小鼠果糖-2,6-二磷酸酶TIGAR(Tigar)ELISA试剂盒酶标板是8×12孔条，可拆卸，板子可以拆成12条，注意避免孔污染，不需要避光和无菌。暂时不用的板子，放回原来装的袋子里，密封保存。

[10].

小鼠果糖-2,6-二磷酸酶TIGAR(Tigar)ELISA试剂盒样本如何保存？

尽量检测新鲜样本。若无新鲜样本，则4℃保存1周，-20℃保存1个月，-80℃保存2个月。

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Mouse Tigar/ Fructose-2,6-bisphosphatase TIGAR ELISA Kit 果糖-2,6-二磷酸酶TIGAR

E5948m

TP53-induced glycolysis and apoptosis regulator, TP53-induced glycolysis regulatory phosphatase

Mouse Tigar/ Fructose-2,6-bisphosphatase TIGAR ELISA Kit
果糖-2,6-二磷酸酶TIGAR