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Inpp5d (基因名), Phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase 1 (蛋白名), SHIP1_MOUSE.

产品名称:

Mouse Inpp5d/ Phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase 1 ELISA Kit
磷脂酰肌醇-3,4,5-六磷铵-磷酸酶1

货号:

E8909m

商标:

EIAab^®

监管等级:

RUO

别名:

Inositol polyphosphate-5-phosphatase D, Inositol polyphosphate-5-phosphatase of 145 kDa, Phosphatidylinositol-4, 5-bisphosphate 5-phosphatase, SH2 domain-containing inositol 5'-phosphatase 1, p150Ship, SIP-145, SH2 domain-containing inositol phosphatase 1, 7a33, Ship, Ship1

检测方法:

ELISA

实验类型:

Competitive

检测范围:

0.78-50ng/ml

灵敏度:

0.403ng/mL

特异性:

Natural and recombinant mouse Phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase 1

样品类型:

Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids

样品数据:

实验步骤:

研究领域:

Cancer

规格 & 价格:

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Mouse Inpp5d ELISA Kit

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产品说明书

数据表:

说明书:

MSDS:

在线询价

数据
文献
序列 / 3D
常见问题解答
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精密度

批内差：已知浓度的3个样本在一个板子内重复检测20次，以评估批内精密度。
批内 CV： ≤6.4%
批间差：已知浓度的3个样本在不同的板子上重复测定5次，以评估测定批间精密度。
批间 CV： ≤8.8%

回收率

回收率：低、中和高浓度的分析物被掺入到血清或者血浆样本中，进行回收实验测定。

Sample Type	Average(%)	Recovery Range(%)
Serum	82	80-88
Plasma	84	80-90

线性

线性：给定样本通过梯度稀释，每次稀释的测量值与理论值的比值。

Sample	1：2	1:4	1:8	1:16
serum(n=5)	87-99%	107-115%	103-113%	86-96%
EDTA plasma(n=5)	95-105%	92-101%	100-109%	106-115%
heparin plasma(n=5)	100-110%	92-102%	99-109%	109-119%

通用注释

亚单元:

Interacts with tyrosine phosphorylated form of SHC1 (PubMed:8654924, PubMed:8643691, PubMed:10068665, PubMed:9083021, PubMed:9099679, PubMed:10395202). Interacts with tyrosine phosphorylated form of DOK1 (PubMed:11031258). Interacts with tyrosine phosphorylated form of DOK3 (PubMed:14993273). Interacts with tyrosine phosphorylated form of SLAMF1/CD150 (By similarity). Interacts with PTPN11/SHP-2 in response to IL-3 (PubMed:9110989, PubMed:9393882). Interacts with receptor EPOR (PubMed:10660611). Interacts with receptors MS4A2/FCER1B and FCER1G (By similarity). Interacts with receptors FCGR2B and FCGR3 (PubMed:10395202, PubMed:11016922, PubMed:10779347, PubMed:15456754, PubMed:12393695). Interacts with receptor FCGR2A, leading to regulate gene expression during the phagocytic process (PubMed:12370370). Interacts with GRB2 (PubMed:8643691, PubMed:10068665). Interacts with PLCG1 (PubMed:16000869). Interacts with tyrosine kinases SRC and TEC (PubMed:10794720, PubMed:15492005). Interacts with CRKL (PubMed:11031258). Interacts with c-Met/MET (PubMed:11896575). Interacts with MILR1 (tyrosine-phosphorylated) (PubMed:20526344). Isoform 5 interacts with IL6ST/gp130 (PubMed:17105399). Can weakly interact (via NPXY motif 2) with DAB2 (via PID domain); the interaction is impaired by tyrosine phosphorylation of the NPXY motif (PubMed:11247302).

功能:

Phosphatidylinositol (PtdIns) phosphatase that specifically hydrolyzes the 5-phosphate of phosphatidylinositol-3,4,5-trisphosphate (PtdIns(3,4,5)P3) to produce PtdIns(3,4)P2, thereby negatively regulating the PI3K (phosphoinositide 3-kinase) pathways (By similarity). Able also to hydrolyzes the 5-phosphate of phosphatidylinositol-4,5-bisphosphate (PtdIns(4,5)P3) and inositol 1,3,4,5-tetrakisphosphate (PubMed:9367159). Acts as a negative regulator of B-cell antigen receptor signaling. Mediates signaling from the FC-gamma-RIIB receptor (FCGR2B), playing a central role in terminating signal transduction from activating immune/hematopoietic cell receptor systems. Acts as a negative regulator of myeloid cell proliferation/survival and chemotaxis, mast cell degranulation, immune cells homeostasis, integrin alpha-IIb/beta-3 signaling in platelets and JNK signaling in B-cells. Regulates proliferation of osteoclast precursors, macrophage programming, phagocytosis and activation and is required for endotoxin tolerance. Involved in the control of cell-cell junctions, CD32a signaling in neutrophils and modulation of EGF-induced phospholipase C activity. Key regulator of neutrophil migration, by governing the formation of the leading edge and polarization required for chemotaxis. Modulates FCGR3/CD16-mediated cytotoxicity in NK cells. Mediates the activin/TGF-beta-induced apoptosis through its Smad-dependent expression.

亚细胞位置:

Isoform 5 Cell membrane Peripheral membrane protein Constitutively present at the cell membrane (PubMed:11567986).

数据库链接

UniGene:

Mm.15105

SMR:

Q9ES52

STRING:

10090.ENSMUSP00000127941

KEGG:

mmu:16331

Pfam:

PF03372

Uniprot:

Q9ES52

相关产品

Human INPP5D ELISA Kit

Rat Inpp5d ELISA Kit

Human INPP5D CLIA Kit

Rat Inpp5d CLIA Kit

Human INPP5D Protein

序列长度:

1191

序列:

仅供参考，详细请咨询（services@eiaab.com）

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EGLLGRTAMQ |

3D 模型:

3D 结构:

免疫原序列:

验证序列:

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[1].

小鼠磷脂酰肌醇-3,4,5-六磷铵-磷酸酶1(Inpp5d)ELISA试剂盒可以做多少个样本？

小鼠磷脂酰肌醇-3,4,5-六磷铵-磷酸酶1(Inpp5d)ELISA试剂盒分为2种规格，96孔和48孔。96孔的试剂盒，标曲和样本都做复孔的话，可以检测40个样本。96孔的试剂盒，标曲和样本都不做复孔的话，可以检测88个样本。

[2].

小鼠磷脂酰肌醇-3,4,5-六磷铵-磷酸酶1(Inpp5d)ELISA试剂盒使用视频？

小鼠磷脂酰肌醇-3,4,5-六磷铵-磷酸酶1(Inpp5d)ELISA试剂盒实验操作视频在以下网址中，对每一步的实验步骤都做了演示，方便实验员能更好地理解ELISA实验的过程。
https://www.eiaab.com.cn/lesson-tech/805.html

[3].

小鼠磷脂酰肌醇-3,4,5-六磷铵-磷酸酶1(Inpp5d)ELISA试剂盒是放在-20℃冰箱保存吗？

EIAab的小鼠磷脂酰肌醇-3,4,5-六磷铵-磷酸酶1(Inpp5d)ELISA试剂盒，洗涤液、底物、终止液保存于4℃，其余试剂-20℃冰箱保存。

[4].

小鼠磷脂酰肌醇-3,4,5-六磷铵-磷酸酶1(Inpp5d)ELISA试剂盒原理？

双抗体夹心法：用纯化的抗体包被微孔板，制成固相抗体，往包被有固相抗体的微孔中依次加入标准品或受检样本、生物素化抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。用酶标仪在450nm波长下测定吸光度(OD值）,计算样本浓度。
竞争法：用纯化的抗体包被微孔板，制成固相抗体，往包被有固相抗体的微孔中依次加入标准品或受检样本和生物素标记的目标分析物，受检标本中抗原与生物素标记抗原竞争结合有限的抗体。再加入HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。用酶标仪在450nm波长下测定吸光度(OD值）,计算样本浓度。

[5].

小鼠磷脂酰肌醇-3,4,5-六磷铵-磷酸酶1(Inpp5d)ELISA试剂盒中需要使用的样品量是多少？

夹心法100μL/孔，竞争法50μL/孔。如样本浓度过高时，应对样本进行稀释，以使稀释后的样本符合试剂盒的检测范围，计算时再乘以相应的稀释倍数。

[6].

如何分析小鼠磷脂酰肌醇-3,4,5-六磷铵-磷酸酶1(Inpp5d)ELISA试剂盒数据？

建议标准曲线，并计算样本浓度。对于elisa的曲线拟合，一般建议采用4参数曲线拟合，4参数曲线拟合通常更适合免疫分析。推荐使用专业软件进行曲线拟合，例如curve expert 1.3。根据样本的OD值由标曲查出相应的浓度，再乘以稀释倍数；或用标准物的浓度与OD值计算出标曲的回归方程式，将样本的OD值代入方程式，计算出样本浓度，再乘以稀释倍数，即为样本的实际浓度。以下链接是curve expert 1.3软件拟合曲线的方法。
https://www.eiaab.com.cn/news/502/

[7].

小鼠磷脂酰肌醇-3,4,5-六磷铵-磷酸酶1(Inpp5d)ELISA试剂盒中是否包含人和动物的副产物，是否包含感染的或者传染性原料如HIV等？

除了抗体和稀释液中的BSA，不含其它人和动物的副产物，也不含感染材料。

[8].

收集小鼠磷脂酰肌醇-3,4,5-六磷铵-磷酸酶1(Inpp5d)ELISA试剂盒血浆样本，用什么作为抗凝剂？

一般建议用EDTA和肝素作为抗凝剂。

[9].

小鼠磷脂酰肌醇-3,4,5-六磷铵-磷酸酶1(Inpp5d)ELISA试剂盒酶标板可以拆成几部分？拆的时候是否需要避光，无菌？

小鼠磷脂酰肌醇-3,4,5-六磷铵-磷酸酶1(Inpp5d)ELISA试剂盒酶标板是8×12孔条，可拆卸，板子可以拆成12条，注意避免孔污染，不需要避光和无菌。暂时不用的板子，放回原来装的袋子里，密封保存。

[10].

小鼠磷脂酰肌醇-3,4,5-六磷铵-磷酸酶1(Inpp5d)ELISA试剂盒样本如何保存？

尽量检测新鲜样本。若无新鲜样本，则4℃保存1周，-20℃保存1个月，-80℃保存2个月。

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Mouse Inpp5d/ Phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase 1 ELISA Kit 磷脂酰肌醇-3,4,5-六磷铵-磷酸酶1

E8909m

Inositol polyphosphate-5-phosphatase D, Inositol polyphosphate-5-phosphatase of 145 kDa, Phosphatidylinositol-4, 5-bisphosphate 5-phosphatase, SH2 domain-containing inositol 5'-phosphatase 1, p150Ship, SIP-145, SH2 domain-containing inositol phosphatase 1, 7a33, Ship, Ship1

Mouse Inpp5d/ Phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase 1 ELISA Kit
磷脂酰肌醇-3,4,5-六磷铵-磷酸酶1