首页 > 重组蛋白 > Human INPP5D Recombinant Protein

INPP5D (基因名), Phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase 1 (蛋白名), ship1_human.

产品名称:

Human INPP5D/ Phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase 1 Recombinant Protein
磷脂酰肌醇-3,4,5-六磷铵-磷酸酶1

货号:

R8909h

商标:

EIAab^®

监管等级:

RUO

别名:

Inositol polyphosphate-5-phosphatase D, Inositol polyphosphate-5-phosphatase of 145 kDa, Phosphatidylinositol 4, 5-bisphosphate 5-phosphatase, SH2 domain-containing inositol 5'-phosphatase 1, p150Ship, SIP-145, SH2 domain-containing inositol phosphatase 1, hp51CN, SHIP, SHIP1

序列号:

Q92835

来源:

E.coli

种属:

Human

标签:

His

序列:

Recombinant protein

纯度:

>90% by SDS-PAGE

浓度:

Reconstitution Dependent

形态:

Liquid

内毒素水平:

Please contact protein@eiaab.com The technician for more information.

应用:

存储缓冲液:

50mM NaH2PO4, 500mM NaCl Buffer with 500mM Imidazole, 10%glycerol(PH8.0)

存储:

Store at -20°C. (Avoid repeated freezing and thawing.)

研究领域:

Cancer

规格 & 价格:

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Human INPP5D Protein

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产品说明书

说明书:

MSDS:

在线询价

数据
文献 ( 1 )
出版物
序列 / 3D
常见问题解答
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R&D 技术数据

更多信息，请参阅手册，或联系我们的技术支持: tech@eiaab.com.

基因位点

Cytogenetic band: 2q37.1 by HGNC 2q37.1 by Entrez Gene 2q37.1 by Ensembl

INPP5D Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)

通用注释

亚单元:

Interacts with tyrosine phosphorylated form of SHC1 (PubMed:8874179). Interacts with tyrosine phosphorylated form of DOK1 (PubMed:10822173). Interacts with tyrosine phosphorylated form of DOK3 (By similarity). Interacts with tyrosine phosphorylated form of SLAMF1/CD150 (PubMed:10229804). Interacts with PTPN11 in response to IL-3 (By similarity). Interacts with receptor EPOR (By similarity). Interacts with receptors MS4A2/FCER1B and FCER1G (By similarity). Interacts with receptors FCGR2B and FCGR3 (By similarity). Interacts with receptor FCGR2A, leading to regulate gene expression during the phagocytic process (By similarity). Interacts with GRB2 (PubMed:8723348, PubMed:9108392). Interacts with PLCG1 (By similarity). Interacts with tyrosine kinases SRC and TEC (By similarity). Interacts with c-Met/MET (By similarity). Interacts with MILR1 (tyrosine-phosphorylated) (By similarity). Can weakly interact (via NPXY motif 2) with DAB2 (via PID domain); the interaction is impaired by tyrosine phosphorylation of the NPXY motif (By similarity). Interacts with FCRL3 and FCRL6 (tyrosine phosphorylated form) (PubMed:20933011, PubMed:19843936).

功能:

Phosphatidylinositol (PtdIns) phosphatase that specifically hydrolyzes the 5-phosphate of phosphatidylinositol-3,4,5-trisphosphate (PtdIns(3,4,5)P3) to produce PtdIns(3,4)P2, thereby negatively regulating the PI3K (phosphoinositide 3-kinase) pathways (PubMed:8723348, PubMed:10764818, PubMed:8769125). Able also to hydrolyzes the 5-phosphate of phosphatidylinositol-4,5-bisphosphate (PtdIns(4,5)P3) and inositol 1,3,4,5-tetrakisphosphate (PubMed:9108392, PubMed:10764818, PubMed:8769125). Acts as a negative regulator of B-cell antigen receptor signaling. Mediates signaling from the FC-gamma-RIIB receptor (FCGR2B), playing a central role in terminating signal transduction from activating immune/hematopoietic cell receptor systems. Acts as a negative regulator of myeloid cell proliferation/survival and chemotaxis, mast cell degranulation, immune cells homeostasis, integrin alpha-IIb/beta-3 signaling in platelets and JNK signaling in B-cells. Regulates proliferation of osteoclast precursors, macrophage programming, phagocytosis and activation and is required for endotoxin tolerance. Involved in the control of cell-cell junctions, CD32a signaling in neutrophils and modulation of EGF-induced phospholipase C activity (PubMed:16682172). Key regulator of neutrophil migration, by governing the formation of the leading edge and polarization required for chemotaxis. Modulates FCGR3/CD16-mediated cytotoxicity in NK cells. Mediates the activin/TGF-beta-induced apoptosis through its Smad-dependent expression.

亚细胞位置:

Cytoplasm Cell membrane Peripheral membrane protein Membrane raft Cytoplasm Cytoskeleton Membrane Peripheral membrane protein Translocates to the plasma membrane when activated, translocation is probably due to different mechanisms depending on the stimulus and cell type. Translocates from the cytoplasm to membrane ruffles in a FCGR3/CD16-dependent manner. Colocalizes with FC-gamma-RIIB receptor (FCGR2B) or FCGR3/CD16 at membrane ruffles. Tyrosine phosphorylation may also participate in membrane localization.

数据库链接

UniGene:

Hs.262886

SMR:

Q92835

STRING:

9606.ENSP00000352575

KEGG:

hsa:3635

MIM:

601582

Pfam:

PF03372

Uniprot:

Q92835

相关产品

Mouse Inpp5d ELISA Kit

Rat Inpp5d ELISA Kit

Mouse Inpp5d CLIA Kit

Rat Inpp5d CLIA Kit

Mouse Inpp5d Protein

序列长度:

1189

序列:

仅供参考，详细请咨询（services@eiaab.com）

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PLGRTAMQ

3D 模型:

3D 结构:

免疫原序列:

验证序列:

[1].

"Phosphatidylinositol-3, 4, 5-Trisphosphate 5-Phosphatase 1: A Meaningful and Independent Marker to Predict Stroke in the Chinese Population"

WJ Tu, XY Liu, H Dong, et al.

From: Journal of Molecular Neuroscience

[1].

"Cloning and characterization of human SHIP, the 145-kD inositol 5-phosphatase that associates with SHC after cytokine stimulation."

(PMID: 8874179)

[2].

"The Src homology 2-containing inositol 5-phosphatase 1 (SHIP1) is involved in CD32a signaling in human neutrophils."

(PMID: 16682172)

[3].

"Evidence that SHIP-1 contributes to phosphatidylinositol 3,4,5-trisphosphate metabolism in T lymphocytes and can regulate novel phosphoinositide 3-kinase effectors."

(PMID: 12421919)

[4].

"The phosphatidylinositol polyphosphate 5-phosphatase SHIP1 associates with the dok1 phosphoprotein in bcr-Abl transformed cells."

(PMID: 10822173)

[5].

"CDw150 associates with src-homology 2-containing inositol phosphatase and modulates CD95-mediated apoptosis."

(PMID: 10229804)

[6].

"The human SHIP gene is differentially expressed in cell lineages of the bone marrow and blood."

(PMID: 9058707)

[7].

"Multiple forms of an inositol polyphosphate 5-phosphatase form signaling complexes with Shc and Grb2."

(PMID: 8723348)

[8].

"Large-scale association analysis identifies 13 new susceptibility loci for coronary artery disease."

(PMID: 21378990)

[9].

"FCRL6 receptor: expression and associated proteins."

(PMID: 20933011)

[10].

"Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study."

(PMID: 20628086)

[1].

人磷脂酰肌醇-3,4,5-六磷铵-磷酸酶1(INPP5D)重组蛋白是否是无菌的？

蛋白试剂瓶和蛋白保存液是经过高压灭菌的，但也不能保证蛋白是完全无菌的。如果要求蛋白是无菌的，可以用0.2微米的滤器对蛋白进行过滤。

[2].

人磷脂酰肌醇-3,4,5-六磷铵-磷酸酶1(INPP5D)重组蛋白的保存缓冲液是什么？

纯化后的蛋白保存在PBS（58mM Na2HPO4, 17mM NaH2PO4, 68mM NaCl, pH7.4）里，并往里面加入500mM咪唑和10%甘油。

[3].

怎样确定人磷脂酰肌醇-3,4,5-六磷铵-磷酸酶1(INPP5D)重组蛋白的浓度？

蛋白浓度的确定没有一个统一的标准，这主要取决于蛋白的氨基酸序列。伊艾博是根据不同测试的组合来测定蛋白浓度。考马斯亮蓝法、BCA法、氨基酸序列和氨基酸全序列分析法等都用来测定蛋白浓度。

[4].

人磷脂酰肌醇-3,4,5-六磷铵-磷酸酶1(INPP5D)重组蛋白蛋白保存条件是怎样的？

蛋白应保存在 -20℃或 -80℃条件下，为了避免反复冻融，可以将蛋白分装成小份保存。

[5].

人磷脂酰肌醇-3,4,5-六磷铵-磷酸酶1(INPP5D)重组蛋白是否可以用于活体实验？

重组蛋白没有用于任何的活体实验，因此蛋白的活性和半衰期是不确定的。

[6].

人磷脂酰肌醇-3,4,5-六磷铵-磷酸酶1(INPP5D)重组蛋白的保质期是多久？

在适当的保存条件下，从购买之日起蛋白可以稳定保存6-12个月。适当的保存条件是:蛋白保存在-20°C o或 -80℃，保证蛋白的保存浓度高于0.1mg/ml，限制蛋白反复冻融的次数。我们公司常规的质量检测保证所有产品在销售时都有可接受的生物活性。但是我们不能控制终端用户蛋白的保存条件。如果产品在有效期内出现问题，请联系我们的技术支持。

[7].

你们蛋白和抗体的报价是怎么样的？

我们将根据你需要的蛋白和抗体的大小进行报价。

[8].

人磷脂酰肌醇-3,4,5-六磷铵-磷酸酶1(INPP5D)重组蛋白是否能够提供蛋白片段？

我们现有的人的蛋白的序列可以有很多。你可以选择你感兴趣的靶向部分，我们将会按您的需求提供蛋白和抗体。

[9].

人磷脂酰肌醇-3,4,5-六磷铵-磷酸酶1(INPP5D)重组蛋白的货期或发货时间一般是多长？

具体指标的货期需要确定。最快一周，最长可能一个月。

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Human INPP5D/ Phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase 1 Recombinant Protein磷脂酰肌醇-3,4,5-六磷铵-磷酸酶1

R8909h

Inositol polyphosphate-5-phosphatase D, Inositol polyphosphate-5-phosphatase of 145 kDa, Phosphatidylinositol 4, 5-bisphosphate 5-phosphatase, SH2 domain-containing inositol 5'-phosphatase 1, p150Ship, SIP-145, SH2 domain-containing inositol phosphatase 1, hp51CN, SHIP, SHIP1

Human INPP5D/ Phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase 1 Recombinant Protein
磷脂酰肌醇-3,4,5-六磷铵-磷酸酶1