Abstract

Aims: This study in bioinformatics aims to investigate the potential effect of Ipomoea tricolor and Sophora tomentosa on liver function enzymes activity, serum lipid profile, oxidative stress biomarkers and on blood glucose in high fat diet-induced hypercholesterolemia and streptozotocin (STZ)-induced hyperglycemia in rats. Methods: :Blood glucose level, liver function enzymes, alanine and aspartate aminotransferases (ALT and AST), alkaline phosphatase (ALP) and lactate dehydrogenase (LDH), were determined. Besides, lipid profile including total cholesterol (TC), triacylglycerol (TG), total lipid and high density lipoprotein –cholesterol (HDL-c) were investigated. Moreover, oxidative stress biomarkers, lipid peroxide and nitric oxide as well as non-enzymatic antioxidant, glutathione (GSH) were also examined in different therapeutic groups. Results :Significant increase in blood glucose level, liver function enzyme activities, LDH, lipid profile and  oxidative  stress markers, while  significant decrease in LDH–c and GSH level in  hypercholesteroemic–hyperglycemic(HC-HG) induced rats  compared to control one. A marked amelioration in all biochemical parameters under investigation upon treatment of  HC-HG rats with I. tricolor and S. tomentosa with different fluctuating percentages of improvement. Histopathological examination  of liver and pancreas was also performed and declared HC-HG showed congestion in portal vessels and sinusoids with mild centrilobular hepatocyte degeneration, marked hepatocyte ballooning and hydropic degeneration while, HC- HG treated rats with I. tricolor and S. tomentosa showed normal lobular hepatic architecture with mild sinusoidal dilatation and congestion. On the other hand, histological organization of  pancreas of HC-HG rats showing disarrangement changes in pancreatic blood vessels and interlobular duct as well as disordered in acini. Treatment of HC-HG rats with  I. tricolor and S. tomentosa showed enhancement  in Langerhans cells  and  restore of  most pancreatic tissue in comparison with standard  drugs. Conclusion :The statistical results showed that each extract ameliorated high blood glucose level liver injury, hypercholesterolemia and  oxidative stress  indicating  relieving of oxidative damage  associated with the complexity of hyperglycemia and hypercholesterolemia. These results demonstrated that these two plants extracts may be a candidate intelligent antioxidant, hypolipidemic, hypoglycemic and hepatoprotective nutraceuticals which need further clinical investigation to be applied effectively to reduce perturbation in hypercholesterolemia associated diabetes.