RLN (基因名), Prorelaxin (蛋白名), relx_pig.
产品名称:
Pig RLN/ Prorelaxin ELISA Kit
Prorelaxin
货号:
E1216p
商标:
EIAab®
监管等级:
检测方法:
ELISA
实验类型:
Sandwich
检测范围:
62.5-4000pg/mL
灵敏度:
31.25pg/mL
特异性:
Natural and recombinant pig Prorelaxin
样品类型:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
样品数据:
登录.
实验步骤:
研究领域:
-
线性
线性:给定样本通过梯度稀释,每次稀释的测量值与理论值的比值。
Sample |
1:2 |
1:4 |
1:8 |
1:16 |
serum(n=5) |
89-101% |
102-114% |
102-112% |
88-97% |
EDTA plasma(n=5) |
83-96% |
100-110% |
95-105% |
101-114% |
heparin plasma(n=5) |
96-107%
|
104-114% |
108-119% |
96-107% |
通用注释
亚单元:
Heterodimer of a B chain and an A chain linked by two disulfide bonds.
功能:
Relaxin is an ovarian hormone that acts with estrogen to produce dilatation of the birth canal in many mammals.
亚细胞位置:
Secreted
数据库链接
UniGene:
SMR:
STRING:
KEGG:
Pfam:
Uniprot:
该产品尚未在任何出版物中被引用。
[1].
猪Prorelaxin(RLN)ELISA试剂盒可以做多少个样本?
猪Prorelaxin(RLN)ELISA试剂盒分为2种规格,96孔和48孔。96孔的试剂盒,标曲和样本都做复孔的话,可以检测40个样本。96孔的试剂盒,标曲和样本都不做复孔的话,可以检测88个样本。
[2].
猪Prorelaxin(RLN)ELISA试剂盒使用视频?
猪Prorelaxin(RLN)ELISA试剂盒实验操作视频在以下网址中,对每一步的实验步骤都做了演示,方便实验员能更好地理解ELISA实验的过程。
https://www.eiaab.com.cn/lesson-tech/805.html
https://www.eiaab.com.cn/lesson-tech/805.html
[3].
猪Prorelaxin(RLN)ELISA试剂盒是放在-20℃冰箱保存吗?
EIAab的猪Prorelaxin(RLN)ELISA试剂盒,洗涤液、底物、终止液保存于4℃,其余试剂-20℃冰箱保存。
[4].
猪Prorelaxin(RLN)ELISA试剂盒原理?
双抗体夹心法:用纯化的抗体包被微孔板,制成固相抗体,往包被有固相抗体的微孔中依次加入标准品或受检样本、生物素化抗体、HRP标记的亲和素,经过彻底洗涤后用底物TMB显色。用酶标仪在450nm波长下测定吸光度(OD值),计算样本浓度。
竞争法:用纯化的抗体包被微孔板,制成固相抗体,往包被有固相抗体的微孔中依次加入标准品或受检样本和生物素标记的目标分析物,受检标本中抗原与生物素标记抗原竞争结合有限的抗体。再加入HRP标记的亲和素,经过彻底洗涤后用底物TMB显色。用酶标仪在450nm波长下测定吸光度(OD值),计算样本浓度。
竞争法:用纯化的抗体包被微孔板,制成固相抗体,往包被有固相抗体的微孔中依次加入标准品或受检样本和生物素标记的目标分析物,受检标本中抗原与生物素标记抗原竞争结合有限的抗体。再加入HRP标记的亲和素,经过彻底洗涤后用底物TMB显色。用酶标仪在450nm波长下测定吸光度(OD值),计算样本浓度。
[5].
猪Prorelaxin(RLN)ELISA试剂盒中需要使用的样品量是多少?
夹心法100μL/孔,竞争法50μL/孔。如样本浓度过高时,应对样本进行稀释,以使稀释后的样本符合试剂盒的检测范围,计算时再乘以相应的稀释倍数。
[6].
如何分析猪Prorelaxin(RLN)ELISA试剂盒数据?
建议标准曲线,并计算样本浓度。对于elisa的曲线拟合,一般建议采用4参数曲线拟合,4参数曲线拟合通常更适合免疫分析。推荐使用专业软件进行曲线拟合,例如curve expert 1.3。根据样本的OD值由标曲查出相应的浓度,再乘以稀释倍数;或用标准物的浓度与OD值计算出标曲的回归方程式,将样本的OD值代入方程式,计算出样本浓度,再乘以稀释倍数,即为样本的实际浓度。以下链接是curve expert 1.3软件拟合曲线的方法。
https://www.eiaab.com.cn/news/502/
https://www.eiaab.com.cn/news/502/
[7].
猪Prorelaxin(RLN)ELISA试剂盒中是否包含人和动物的副产物,是否包含感染的或者传染性原料如HIV等?
除了抗体和稀释液中的BSA,不含其它人和动物的副产物,也不含感染材料。
[8].
收集猪Prorelaxin(RLN)ELISA试剂盒血浆样本,用什么作为抗凝剂?
一般建议用EDTA和肝素作为抗凝剂。
[9].
猪Prorelaxin(RLN)ELISA试剂盒酶标板可以拆成几部分?拆的时候是否需要避光,无菌?
猪Prorelaxin(RLN)ELISA试剂盒酶标板是8×12孔条,可拆卸,板子可以拆成12条,注意避免孔污染,不需要避光和无菌。暂时不用的板子,放回原来装的袋子里,密封保存。
[10].
猪Prorelaxin(RLN)ELISA试剂盒样本如何保存?
尽量检测新鲜样本。若无新鲜样本,则4℃保存1周,-20℃保存1个月,-80℃保存2个月。
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porcine relaxin ELISA kit
I am interested in the porcine relaxin ELISA kit (E1216p). Can you send me the quotation and the details? In addition, can you please let me know of the source of the porcine relaxin antibody.
customer
2015-02-01 21:50
2015-02-01 21:50
Dear April,
The price is USD900, additionally the shipping fee USD50 per kit. If a Express account provided, you do not need charge the shipping fee.
As to our company's rule, the purchasing way is that 1 you send us an order (email), we send you the Proforma Invoice 2 you prepay us by T/T (wire transfer) 3 we send the products to you directly from China. And we will arrange the shipment via FedEx or UPS in 3-5 workings as soon as we receive your payment. And you can receive it in 3-5 working days after the shipment.
The source of the antibody used in the kit is polyclonal from rabbit.
If you have further questions please do not hesitate to get back to us.
star
2015-02-02 17:55 最佳回复
2015-02-02 17:55 最佳回复
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0
I cannot differentiate spiked control (porcine relaxin) sample versus blank sample. Therefore the sensitivity is very bad.
Since we are only testing the ELISA kit, will there be a discount?
customer
2015-02-02 18:02
2015-02-02 18:02
0
0
The sensitivity is 31.2pg/ml.
The specificity: recognizes recombinant and natural Pig Prorelaxin.
The antibodies is produced by our company.
Did you purchased the kit from our company?What is the problem?
star
2015-02-02 18:00
2015-02-02 18:00
0
0
Can you please give me the details on the sensitivity and specificity. Are the antibodies attached on the ELISA manufactured in-house or supplied by other collaborators? The reason I’m asking is that I have tried a porcine relaxin ELISA a few years ago, however, it does not work.
customer
2015-02-02 17:58
2015-02-02 17:58
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