首页 > 重组蛋白 > Human EZH2 Recombinant Protein

EZH2 (基因名), Histone-lysine N-methyltransferase EZH2 (蛋白名), ezh2_human.

产品名称:

Human EZH2/ Histone-lysine N-methyltransferase EZH2 Recombinant Protein
Histone-lysine N-methyltransferase EZH2

货号:

R7688h

商标:

EIAab^®

监管等级:

RUO

别名:

ENX-1, Enhancer of zeste homolog 2, Lysine N-methyltransferase 6, KMT6

序列号:

Q15910

来源:

E.coli

种属:

Human

标签:

His

纯度:

>90% by SDS-PAGE

浓度:

Reconstitution Dependent

形态:

Liquid

内毒素水平:

Please contact protein@eiaab.com The technician for more information.

应用:

存储缓冲液:

50mM NaH2PO4, 500mM NaCl Buffer with 500mM Imidazole, 10%glycerol(PH8.0)

存储:

Store at -20°C. (Avoid repeated freezing and thawing.)

研究领域:

Epigenetics

规格 & 价格:

关注

Human EZH2 Protein

邮箱 *

消息 *

Please 登录.

产品说明书

说明书:

MSDS:

在线询价

数据
文献
出版物
序列 / 3D
常见问题解答
反馈墙 (0)

R&D 技术数据

更多信息，请参阅手册，或联系我们的技术支持: tech@eiaab.com.

基因位点

Cytogenetic band: 7q36.1 by HGNC 7q36.1 by Entrez Gene 7q36.1 by Ensembl

EZH2 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)

通用注释

亚单元:

Binds ATRX via the SET domain (Probable). Component of the PRC2/EED-EZH2 complex, which includes EED, EZH2, SUZ12, RBBP4 and RBBP7 and possibly AEBP2. The minimum components required for methyltransferase activity of the PRC2/EED-EZH2 complex are EED, EZH2 and SUZ12. The PRC2 complex may also interact with DNMT1, DNMT3A, DNMT3B and PHF1 via the EZH2 subunit and with SIRT1 via the SUZ12 subunit. Interacts with HDAC1 and HDAC2. Interacts with PRAME. Interacts with CDYL. Interacts with CLOCK, ARNTL/BMAL1 and CRY1 (By similarity). Interacts with DNMT3L; the interaction is direct.

功能:

Polycomb group (PcG) protein. Catalytic subunit of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' (H3K9me) and 'Lys-27' (H3K27me) of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate 'Lys-27' of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Compared to EZH2-containing complexes, it is more abundant in embryonic stem cells and plays a major role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1, CDKN2A and retinoic acid target genes. EZH2 can also methylate non-histone proteins such as the transcription factor GATA4 and the nuclear receptor RORA. Regulates the circadian clock via histone methylation at the promoter of the circadian genes. Essential for the CRY1/2-mediated repression of the transcriptional activation of PER1/2 by the CLOCK-ARNTL/BMAL1 heterodimer; involved in the di and trimethylation of 'Lys-27' of histone H3 on PER1/2 promoters which is necessary for the CRY1/2 proteins to inhibit transcription.

亚细胞位置:

Nucleus

数据库链接

UniGene:

Hs.444082

SMR:

Q15910

STRING:

9606.ENSP00000320147

KEGG:

hsa:2146

MIM:

277590

Pfam:

PF11616

Uniprot:

Q15910

相关产品

Mouse Ezh2 ELISA Kit

Mouse Ezh2 CLIA Kit

Mouse Ezh2 Protein

Mouse Ezh2 Polyclonal Antibody

Mouse Ezh2 Monoclonal Antibody

序列长度:

746

序列:

仅供参考，详细请咨询（services@eiaab.com）

50:

100:

150:

200:

250:

300:

350:

400:

450:

500:

550:

600:

650:

700:

746:

MVNGDHRIGI | FAKRAIQTGE | ELFFDYRYSQ | ADALKYVGIE | REMEIP

3D 模型:

3D 结构:

免疫原序列:

验证序列:

该产品尚未在任何出版物中被引用。

[1].

"Cloning of a human homolog of the Drosophila enhancer of zeste gene (EZH2) that maps to chromosome 21q22.2."

(PMID: 8954776)

[2].

"Somatic mutations altering EZH2 (Tyr641) in follicular and diffuse large B-cell lymphomas of germinal-center origin."

(PMID: 20081860)

[3].

"Genetic polymorphisms in the polycomb group gene EZH2 and the risk of lung cancer."

(PMID: 19901851)

[4].

"Ezh2 requires PHF1 to efficiently catalyze H3 lysine 27 trimethylation in vivo."

(PMID: 18285464)

[5].

"Histone deacetylase inhibitors deplete enhancer of zeste 2 and associated polycomb repressive complex 2 proteins in human acute leukemia cells."

(PMID: 17172412)

[6].

"The Polycomb group protein EZH2 directly controls DNA methylation."

(PMID: 16357870)

[7].

"Akt-mediated phosphorylation of EZH2 suppresses methylation of lysine 27 in histone H3."

(PMID: 16224021)

[8].

"Mutation screen and association study of EZH2 as a susceptibility gene for aggressive prostate cancer."

(PMID: 16015586)

[9].

"Activated p53 suppresses the histone methyltransferase EZH2 gene."

(PMID: 15208672)

[10].

"EZH2 is downstream of the pRB-E2F pathway, essential for proliferation and amplified in cancer."

(PMID: 14532106)

[1].

人Histone-lysine N-methyltransferase EZH2(EZH2)重组蛋白是否是无菌的？

蛋白试剂瓶和蛋白保存液是经过高压灭菌的，但也不能保证蛋白是完全无菌的。如果要求蛋白是无菌的，可以用0.2微米的滤器对蛋白进行过滤。

[2].

人Histone-lysine N-methyltransferase EZH2(EZH2)重组蛋白的保存缓冲液是什么？

纯化后的蛋白保存在PBS（58mM Na2HPO4, 17mM NaH2PO4, 68mM NaCl, pH7.4）里，并往里面加入500mM咪唑和10%甘油。

[3].

怎样确定人Histone-lysine N-methyltransferase EZH2(EZH2)重组蛋白的浓度？

蛋白浓度的确定没有一个统一的标准，这主要取决于蛋白的氨基酸序列。伊艾博是根据不同测试的组合来测定蛋白浓度。考马斯亮蓝法、BCA法、氨基酸序列和氨基酸全序列分析法等都用来测定蛋白浓度。

[4].

人Histone-lysine N-methyltransferase EZH2(EZH2)重组蛋白蛋白保存条件是怎样的？

蛋白应保存在 -20℃或 -80℃条件下，为了避免反复冻融，可以将蛋白分装成小份保存。

[5].

人Histone-lysine N-methyltransferase EZH2(EZH2)重组蛋白是否可以用于活体实验？

重组蛋白没有用于任何的活体实验，因此蛋白的活性和半衰期是不确定的。

[6].

人Histone-lysine N-methyltransferase EZH2(EZH2)重组蛋白的保质期是多久？

在适当的保存条件下，从购买之日起蛋白可以稳定保存6-12个月。适当的保存条件是:蛋白保存在-20°C o或 -80℃，保证蛋白的保存浓度高于0.1mg/ml，限制蛋白反复冻融的次数。我们公司常规的质量检测保证所有产品在销售时都有可接受的生物活性。但是我们不能控制终端用户蛋白的保存条件。如果产品在有效期内出现问题，请联系我们的技术支持。

[7].

你们蛋白和抗体的报价是怎么样的？

我们将根据你需要的蛋白和抗体的大小进行报价。

[8].

人Histone-lysine N-methyltransferase EZH2(EZH2)重组蛋白是否能够提供蛋白片段？

我们现有的人的蛋白的序列可以有很多。你可以选择你感兴趣的靶向部分，我们将会按您的需求提供蛋白和抗体。

[9].

人Histone-lysine N-methyltransferase EZH2(EZH2)重组蛋白的货期或发货时间一般是多长？

具体指标的货期需要确定。最快一周，最长可能一个月。

反馈墙

评论数 : 0

所有用户