首页 > 重组蛋白 > Human MSH6 Recombinant Protein

MSH6 (基因名), DNA mismatch repair protein Msh6 (蛋白名), MSH6_HUMAN.

产品名称:

Human MSH6/ DNA mismatch repair protein Msh6 Recombinant Protein
DNA错配修复蛋白Msh6

货号:

R2360h

商标:

EIAab^®

监管等级:

RUO

别名:

G/T mismatch-binding protein, MutS protein homolog 6, MutS-alpha 160 kDa subunit, GTBP, p160, hMSH6, GTBP

序列号:

P52701

来源:

E.coli

种属:

Human

标签:

His

纯度:

>90% by SDS-PAGE

浓度:

Reconstitution Dependent

形态:

Liquid

内毒素水平:

Please contact protein@eiaab.com The technician for more information.

应用:

存储缓冲液:

50mM NaH2PO4, 500mM NaCl Buffer with 500mM Imidazole, 10%glycerol(PH8.0)

存储:

Store at -20°C. (Avoid repeated freezing and thawing.)

研究领域:

Cancer

规格 & 价格:

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Human MSH6 Protein

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产品说明书

说明书:

MSDS:

在线询价

数据
文献
出版物
序列 / 3D
常见问题解答
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R&D 技术数据

更多信息，请参阅手册，或联系我们的技术支持: tech@eiaab.com.

基因位点

Cytogenetic band: 2p16.3 by HGNC 2p16.3 by Entrez Gene 2p16.3 by Ensembl

MSH6 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)

通用注释

亚单元:

Heterodimer consisting of MSH2-MSH6 (MutS alpha). Forms a ternary complex with MutL alpha (MLH1-PMS1). Interacts with EXO1. Part of the BRCA1-associated genome surveillance complex (BASC), which contains BRCA1, MSH2, MSH6, MLH1, ATM, BLM, PMS2 and the RAD50-MRE11-NBS1 protein complex. This association could be a dynamic process changing throughout the cell cycle and within subnuclear domains. Interacts with ATR. Interacts with herpes simplex virus 1 protein UL12 (PubMed:21957315).

功能:

Component of the post-replicative DNA mismatch repair system (MMR). Heterodimerizes with MSH2 to form MutS alpha, which binds to DNA mismatches thereby initiating DNA repair. When bound, MutS alpha bends the DNA helix and shields approximately 20 base pairs, and recognizes single base mismatches and dinucleotide insertion-deletion loops (IDL) in the DNA. After mismatch binding, forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. ATP binding and hydrolysis play a pivotal role in mismatch repair functions. The ATPase activity associated with MutS alpha regulates binding similar to a molecular switch: mismatched DNA provokes ADP-->ATP exchange, resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone. This transition is crucial for mismatch repair. MutS alpha may also play a role in DNA homologous recombination repair. Recruited on chromatin in G1 and early S phase via its PWWP domain that specifically binds trimethylated 'Lys-36' of histone H3 (H3K36me3): early recruitment to chromatin to be replicated allowing a quick identification of mismatch repair to initiate the DNA mismatch repair reaction.

亚细胞位置:

Nucleus Chromosome Associates with H3K36me3 via its PWWP domain.

数据库链接

UniGene:

Hs.445052

SMR:

P52701

STRING:

9606.ENSP00000234420

KEGG:

hsa:2956

MIM:

114500

Pfam:

PF01624

Uniprot:

P52701

相关产品

Mouse Msh6 ELISA Kit

Chicken MSH6 ELISA Kit

Mouse Msh6 CLIA Kit

Chicken MSH6 CLIA Kit

Mouse Msh6 Protein

序列长度:

1360

序列:

仅供参考，详细请咨询（services@eiaab.com）

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HKLLTLIKEL |

3D 模型:

3D 结构:

免疫原序列:

验证序列:

该产品尚未在任何出版物中被引用。

[1].

"Mutation analysis of the MLH1, MSH2 and MSH6 genes in patients with double primary cancers of the colorectum and the endometrium: a population-based study in northern Sweden."

(PMID: 14961575)

[2].

"MSH6 germline mutations are rare in colorectal cancer families."

(PMID: 14520694)

[3].

"Mutations of GTBP in genetically unstable cells."

(PMID: 7604266)

[4].

"Screening for germline mutations of MLH1, MSH2, MSH6 and PMS2 genes in Slovenian colorectal cancer patients: implications for a population specific detection strategy of Lynch syndrome."

(PMID: 19526325)

[5].

"Cancer risk in MLH1, MSH2 and MSH6 mutation carriers; different risk profiles may influence clinical management."

(PMID: 20028567)

[6].

"Meta-analysis of MSH6 gene mutation frequency in colorectal and endometrial cancers."

(PMID: 19492230)

[7].

"Association of MUTYH and MSH6 germline mutations in colorectal cancer patients."

(PMID: 19685280)

[8].

"Classification of ambiguous mutations in DNA mismatch repair genes identified in a population-based study of colorectal cancer."

(PMID: 18033691)

[9].

"Detection of mismatch repair gene germline mutation carrier among Chinese population with colorectal cancer."

(PMID: 18257912)

[10].

"Germline MSH6 mutations are more prevalent in endometrial cancer patient cohorts than hereditary non polyposis colorectal cancer cohorts."

(PMID: 18269114)

[1].

人DNA错配修复蛋白Msh6(MSH6)重组蛋白是否是无菌的？

蛋白试剂瓶和蛋白保存液是经过高压灭菌的，但也不能保证蛋白是完全无菌的。如果要求蛋白是无菌的，可以用0.2微米的滤器对蛋白进行过滤。

[2].

人DNA错配修复蛋白Msh6(MSH6)重组蛋白的保存缓冲液是什么？

纯化后的蛋白保存在PBS（58mM Na2HPO4, 17mM NaH2PO4, 68mM NaCl, pH7.4）里，并往里面加入500mM咪唑和10%甘油。

[3].

怎样确定人DNA错配修复蛋白Msh6(MSH6)重组蛋白的浓度？

蛋白浓度的确定没有一个统一的标准，这主要取决于蛋白的氨基酸序列。伊艾博是根据不同测试的组合来测定蛋白浓度。考马斯亮蓝法、BCA法、氨基酸序列和氨基酸全序列分析法等都用来测定蛋白浓度。

[4].

人DNA错配修复蛋白Msh6(MSH6)重组蛋白蛋白保存条件是怎样的？

蛋白应保存在 -20℃或 -80℃条件下，为了避免反复冻融，可以将蛋白分装成小份保存。

[5].

人DNA错配修复蛋白Msh6(MSH6)重组蛋白是否可以用于活体实验？

重组蛋白没有用于任何的活体实验，因此蛋白的活性和半衰期是不确定的。

[6].

人DNA错配修复蛋白Msh6(MSH6)重组蛋白的保质期是多久？

在适当的保存条件下，从购买之日起蛋白可以稳定保存6-12个月。适当的保存条件是:蛋白保存在-20°C o或 -80℃，保证蛋白的保存浓度高于0.1mg/ml，限制蛋白反复冻融的次数。我们公司常规的质量检测保证所有产品在销售时都有可接受的生物活性。但是我们不能控制终端用户蛋白的保存条件。如果产品在有效期内出现问题，请联系我们的技术支持。

[7].

你们蛋白和抗体的报价是怎么样的？

我们将根据你需要的蛋白和抗体的大小进行报价。

[8].

人DNA错配修复蛋白Msh6(MSH6)重组蛋白是否能够提供蛋白片段？

我们现有的人的蛋白的序列可以有很多。你可以选择你感兴趣的靶向部分，我们将会按您的需求提供蛋白和抗体。

[9].

人DNA错配修复蛋白Msh6(MSH6)重组蛋白的货期或发货时间一般是多长？

具体指标的货期需要确定。最快一周，最长可能一个月。

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