首页 > 重组蛋白 > Human CDC42BPB Recombinant Protein

CDC42BPB (基因名), Serine/threonine-protein kinase MRCK beta (蛋白名), MRCKB_HUMAN.

产品名称:

Human CDC42BPB/ Serine/threonine-protein kinase MRCK beta Recombinant Protein

货号:

商标:

EIAab^®

监管等级:

RUO

别名:

CDC42-binding protein kinase beta, DMPK-like beta, Myotonic dystrophy kinase-related CDC42-binding kinase beta, CDC42BP-beta, MRCK beta, KIAA1124

序列号:

Q9Y5S2

来源:

E.coli

种属:

Human

标签:

His

纯度:

>90% by SDS-PAGE

浓度:

Reconstitution Dependent

形态:

Liquid

内毒素水平:

Please contact protein@eiaab.com The technician for more information.

应用:

存储缓冲液:

50mM NaH2PO4, 500mM NaCl Buffer with 500mM Imidazole, 10%glycerol(PH8.0)

存储:

Store at -20°C. (Avoid repeated freezing and thawing.)

研究领域:

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Human CDC42BPB Protein

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产品说明书

说明书:

MSDS:

在线询价

数据
文献
出版物
序列 / 3D
常见问题解答
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基因位点

Cytogenetic band: 14q32.32 by HGNC 14q32.32 by Entrez Gene 14q32.32 by Ensembl

CDC42BPB Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)

通用注释

亚单元:

Homodimer and homotetramer via the coiled coil regions (PubMed:21949762). Interacts tightly with GTP-bound but not GDP-bound CDC42. Interacts with TJP1, when in the presence of catalytically active CDC42 (By similarity). Forms a tripartite complex with MYO18A and LURAP1 with the latter acting as an adapter connecting CDC42BPB and MYO18A. LURAP1 binding results in activation of CDC42BPB by abolition of its negative autoregulation (PubMed:18854160). Interacts with STRIP1, STRN3 and SIKE1 (PubMed:25743393). Interacts with CPNE4 (via VWFA domain).

功能:

Serine/threonine-protein kinase which is an important downstream effector of CDC42 and plays a role in the regulation of cytoskeleton reorganization and cell migration. Regulates actin cytoskeletal reorganization via phosphorylation of PPP1R12C and MYL9/MLC2. In concert with MYO18A and LURAP1, is involved in modulating lamellar actomyosin retrograde flow that is crucial to cell protrusion and migration. Phosphorylates PPP1R12A.

亚细胞位置:

Cytoplasm Cell membrane Peripheral membrane protein Cytoplasmic side Cell junction Displays a dispersed punctate distribution and concentrates along the cell periphery, especially at the leading edge and cell-cell junction. This concentration is PH-domain dependent (By similarity). Detected at the leading edge of migrating cells. Localization at the leading edge of migrating cells requires interaction with catalytically active CDC42.

数据库链接

UniGene:

Hs.654634

SMR:

Q9Y5S2

STRING:

9606.ENSP00000355237

KEGG:

hsa:9578

MIM:

614062

Pfam:

PF00130

Uniprot:

Q9Y5S2

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序列长度:

1711

序列:

仅供参考，详细请咨询（services@eiaab.com）

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LEGLEQPACD | T

3D 模型:

3D 结构:

免疫原序列:

验证序列:

该产品尚未在任何出版物中被引用。

[1].

"Cloning and chromosomal localization of human Cdc42-binding protein kinase beta."

(PMID: 10198171)

[2].

"CCM-3/STRIPAK promotes seamless tube extension through endocytic recycling."

(PMID: 25743393)

[3].

"Co-crystal structures of inhibitors with MRCKβ, a key regulator of tumor cell invasion."

(PMID: 21949762)

[4].

"Common variants on 14q32 and 13q12 are associated with DLBCL susceptibility."

(PMID: 21471979)

[5].

"Cdc42-dependent formation of the ZO-1/MRCKβ complex at the leading edge controls cell migration."

(PMID: 21240187)

[1].

人CDC42BPB重组蛋白是否是无菌的？

蛋白试剂瓶和蛋白保存液是经过高压灭菌的，但也不能保证蛋白是完全无菌的。如果要求蛋白是无菌的，可以用0.2微米的滤器对蛋白进行过滤。

[2].

人CDC42BPB重组蛋白的保存缓冲液是什么？

纯化后的蛋白保存在PBS（58mM Na2HPO4, 17mM NaH2PO4, 68mM NaCl, pH7.4）里，并往里面加入500mM咪唑和10%甘油。

[3].

怎样确定人CDC42BPB重组蛋白的浓度？

蛋白浓度的确定没有一个统一的标准，这主要取决于蛋白的氨基酸序列。伊艾博是根据不同测试的组合来测定蛋白浓度。考马斯亮蓝法、BCA法、氨基酸序列和氨基酸全序列分析法等都用来测定蛋白浓度。

[4].

人CDC42BPB重组蛋白蛋白保存条件是怎样的？

蛋白应保存在 -20℃或 -80℃条件下，为了避免反复冻融，可以将蛋白分装成小份保存。

[5].

人CDC42BPB重组蛋白是否可以用于活体实验？

重组蛋白没有用于任何的活体实验，因此蛋白的活性和半衰期是不确定的。

[6].

人CDC42BPB重组蛋白的保质期是多久？

在适当的保存条件下，从购买之日起蛋白可以稳定保存6-12个月。适当的保存条件是:蛋白保存在-20°C o或 -80℃，保证蛋白的保存浓度高于0.1mg/ml，限制蛋白反复冻融的次数。我们公司常规的质量检测保证所有产品在销售时都有可接受的生物活性。但是我们不能控制终端用户蛋白的保存条件。如果产品在有效期内出现问题，请联系我们的技术支持。

[7].

你们蛋白和抗体的报价是怎么样的？

我们将根据你需要的蛋白和抗体的大小进行报价。

[8].

人CDC42BPB重组蛋白是否能够提供蛋白片段？

我们现有的人的蛋白的序列可以有很多。你可以选择你感兴趣的靶向部分，我们将会按您的需求提供蛋白和抗体。

[9].

人CDC42BPB重组蛋白的货期或发货时间一般是多长？

具体指标的货期需要确定。最快一周，最长可能一个月。

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