MOCS3 (基因名), Adenylyltransferase and sulfurtransferase MOCS3 (蛋白名), MOCS3_HUMAN.
Human MOCS3/ Adenylyltransferase and sulfurtransferase MOCS3 Recombinant Protein
Molybdenum cofactor synthesis protein 3, Molybdopterin synthase sulfurylase, MPT synthase sulfurylase, UBA4
>90% by SDS-PAGE
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50mM NaH2PO4, 500mM NaCl Buffer with 500mM Imidazole, 10%glycerol(PH8.0)
Store at -20°C. (Avoid repeated freezing and thawing.)
Interacts with NFS1.
Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of cytosolic tRNA(Lys), tRNA(Glu) and tRNA(Gln). Also essential during biosynthesis of the molybdenum cofactor. Acts by mediating the C-terminal thiocarboxylation of sulfur carriers URM1 and MOCS2A. Its N-terminus first activates URM1 and MOCS2A as acyl-adenylates (-COAMP), then the persulfide sulfur on the catalytic cysteine is transferred to URM1 and MOCS2A to form thiocarboxylation (-COSH) of their C-terminus. The reaction probably involves hydrogen sulfide that is generated from the persulfide intermediate and that acts as nucleophile towards URM1 and MOCS2A. Subsequently, a transient disulfide bond is formed. Does not use thiosulfate as sulfur donor; NFS1 probably acting as a sulfur donor for thiocarboxylation reactions.
MASREEVLAL | QAEVAQREEE | LNSLKQKLAS | ALLAEQEPQP | ERLVPVSPLP |
PKAALSRDEI | LRYSRQLVLP | ELGVHGQLRL | GTACVLIVGC | GGLGCPLAQY |
LAAAGVGRLG | LVDYDVVEMS | NLARQVLHGE | ALAGQAKAFS | AAASLRRLNS |
AVECVPYTQA | LTPATALDLV | RRYDVVADCS | DNVPTRYLVN | DACVLAGRPL |
VSASALRFEG | QITVYHYDGG | PCYRCIFPQP | PPAETVTNCA | DGGVLGVVTG |
VLGCLQALEV | LKIAAGLGPS | YSGSLLLFDA | LRGHFRSIRL | RSRRLDCAAC |
GERPTVTDLL | DYEAFCGSSA | TDKCRSLQLL | SPEERVSVTD | YKRLLDSGAF |
HLLLDVRPQV | EVDICRLPHA | LHIPLKHLER | RDAESLKLLK | EAIWEEKQGT |
QEGAAVPIYV | ICKLGNDSQK | AVKILQSLSA | AQELDPLTVR | DVVGGLMAWA |
"Site-directed mutagenesis of the active site loop of the rhodanese-like domain of the human molybdopterin synthase sulfurase MOCS3. Major differences in substrate specificity between eukaryotic and bacterial homologs."
"Molybdenum cofactor biosynthesis in humans: identification of a persulfide group in the rhodanese-like domain of MOCS3 by mass spectrometry."
"Evidence for the physiological role of a rhodanese-like protein for the biosynthesis of the molybdenum cofactor in humans."
"The sulfurtransferase activity of Uba4 presents a link between ubiquitin-like protein conjugation and activation of sulfur carrier proteins."
"A novel role for human Nfs1 in the cytoplasm: Nfs1 acts as a sulfur donor for MOCS3, a protein involved in molybdenum cofactor biosynthesis."
"A functional proteomics approach links the ubiquitin-related modifier Urm1 to a tRNA modification pathway."
"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
"Mutations in the molybdenum cofactor biosynthetic genes MOCS1, MOCS2, and GEPH."
"The DNA sequence and comparative analysis of human chromosome 20."
"Architecture of the human interactome defines protein communities and disease networks."
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