MMP9 (基因名), Matrix metalloproteinase-9 (蛋白名), MMP9_HUMAN.
产品名称:
Human MMP9/ Matrix metalloproteinase-9 Recombinant Protein
矩阵metalloproteinase-9
货号:
R0553h
商标:
EIAab®
监管等级:
别名:
92 kDa gelatinase, 92 kDa type IV collagenase, Gelatinase B, GELB, MMP-9, CLG4B
序列号:
P14780
来源:
E.coli
种属:
Human
标签:
His
序列:
161-320aa
预估分子量:
17.6 kDa (monomer)
纯度:
Greater than 98% by SDS-PAGE
浓度:
Reconstitution Dependent
形态:
Liquid
内毒素水平:
Please contact protein@eiaab.com The technician for more information.
应用:
存储缓冲液:
50mM NaH2PO4, 500mM NaCl Buffer with 500mM Imidazole, 10%glycerol(PH8.0)
存储:
Store at -20°C. (Avoid repeated freezing and thawing.)
研究领域:
Cancer
R&D 技术数据
The PCR product of human MMP9 gene was determined by 1% Agarose stained with EB.
Recombinant human MMP9 protein was determined by 12% SDS-PAGE stained with Coomassie Blue under reducing conditions.
基因位点
MMP9 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
通用注释
亚单元:
Exists as monomer or homodimer; disulfide-linked. Exists also as heterodimer with a 25 kDa protein. Macrophages and transformed cell lines produce only the monomeric form. Interacts with ECM1.
功能:
May play an essential role in local proteolysis of the extracellular matrix and in leukocyte migration. Could play a role in bone osteoclastic resorption. Cleaves KiSS1 at a Gly-|-Leu bond. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments. Degrades fibronectin but not laminin or Pz-peptide.
亚细胞位置:
Secreted
Extracellular space
Extracellular matrix
数据库链接
验证序列:
[1].
Haiwei Wu, Guohua Dong, Hao Liu, Biao Xu, Demin Li, Hua Jing
[2].
[1].
"Association of matrix metalloproteinase 9 genotypes and cardiovascular disease risk factors with serum matrix metalloproteinase 9 concentrations in Taiwanese individuals."
[2].
"A functional polymorphism in THBS2 that affects alternative splicing and MMP binding is associated with lumbar-disc herniation."
[3].
"Promoter polymorphisms in matrix metalloproteinases and their inhibitors: few associations with breast cancer susceptibility and progression."
[4].
"Influence of MMP-2 and MMP-9 promoter polymorphisms on gene expression and clinical outcome of non-small cell lung cancer."
[5].
"Analysis of MMP-1 and MMP-9 promoter polymorphisms in early osseointegrated implant failure."
[6].
"Matrix metalloproteinase 9 (MMP-9) gene polymorphism and MMP-9 plasma levels in primary Sjogren's syndrome."
[7].
"Interferon gamma receptor 2 gene variants are associated with liver fibrosis in patients with chronic hepatitis C infection."
[8].
"Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study."
[10].
"Genetic risk factors for hepatopulmonary syndrome in patients with advanced liver disease."
[1].
人矩阵metalloproteinase-9(MMP9)重组蛋白是否是无菌的？
蛋白试剂瓶和蛋白保存液是经过高压灭菌的，但也不能保证蛋白是完全无菌的。如果要求蛋白是无菌的，可以用0.2微米的滤器对蛋白进行过滤。
[2].
人矩阵metalloproteinase-9(MMP9)重组蛋白的保存缓冲液是什么？
纯化后的蛋白保存在PBS（58mM Na2HPO4, 17mM NaH2PO4, 68mM NaCl, pH7.4）里，并往里面加入500mM咪唑和10%甘油。
[3].
怎样确定人矩阵metalloproteinase-9(MMP9)重组蛋白的浓度？
蛋白浓度的确定没有一个统一的标准，这主要取决于蛋白的氨基酸序列。伊艾博是根据不同测试的组合来测定蛋白浓度。考马斯亮蓝法、BCA法、氨基酸序列和氨基酸全序列分析法等都用来测定蛋白浓度。
[4].
人矩阵metalloproteinase-9(MMP9)重组蛋白蛋白保存条件是怎样的？
蛋白应保存在 -20℃或 -80℃条件下，为了避免反复冻融，可以将蛋白分装成小份保存。
[5].
人矩阵metalloproteinase-9(MMP9)重组蛋白是否可以用于活体实验？
重组蛋白没有用于任何的活体实验，因此蛋白的活性和半衰期是不确定的。
[6].
人矩阵metalloproteinase-9(MMP9)重组蛋白的保质期是多久？
在适当的保存条件下，从购买之日起蛋白可以稳定保存6-12个月。适当的保存条件是:蛋白保存在-20°C o或 -80℃，保证蛋白的保存浓度高于0.1mg/ml，限制蛋白反复冻融的次数。我们公司常规的质量检测保证所有产品在销售时都有可接受的生物活性。但是我们不能控制终端用户蛋白的保存条件。如果产品在有效期内出现问题，请联系我们的技术支持。
[7].
你们蛋白和抗体的报价是怎么样的？
我们将根据你需要的蛋白和抗体的大小进行报价。
[8].
人矩阵metalloproteinase-9(MMP9)重组蛋白是否能够提供蛋白片段？
我们现有的人的蛋白的序列可以有很多。你可以选择你感兴趣的靶向部分，我们将会按您的需求提供蛋白和抗体。
[9].
人矩阵metalloproteinase-9(MMP9)重组蛋白的货期或发货时间一般是多长？
具体指标的货期需要确定。最快一周，最长可能一个月。
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