首页 > 重组蛋白 > Human MDK Recombinant Protein

MDK (基因名), Midkine (蛋白名), MK_HUMAN.

产品名称:

Human MDK/ Midkine Recombinant Protein
肝素结合细胞因子

货号:

R0631h

商标:

EIAab^®

监管等级:

RUO

别名:

Amphiregulin-associated protein, Midgestation and kidney protein, Neurite outgrowth-promoting factor 2, Neurite outgrowth-promoting protein, ARAP, MK, MK1, NEGF2

序列号:

P21741

来源:

E.coli

种属:

Human

标签:

His

序列:

21-143aa

预估分子量:

13.53 kDa (monomer)

纯度:

Greater than 95% by SDS-PAGE

浓度:

Reconstitution Dependent

形态:

Liquid

内毒素水平:

Please contact protein@eiaab.com The technician for more information.

应用:

存储缓冲液:

50mM NaH2PO4, 500mM NaCl Buffer with 500mM Imidazole, 10%glycerol(PH8.0)

存储:

Store at -20°C. (Avoid repeated freezing and thawing.)

研究领域:

Neurosciences

规格 & 价格:

关注

Human MDK Protein

邮箱 *

消息 *

Please 登录.

产品说明书

说明书:

MSDS:

在线询价

数据
文献 ( 1 )
出版物
序列 / 3D
常见问题解答
反馈墙 (0)

R&D 技术数据

更多信息，请参阅手册，或联系我们的技术支持: tech@eiaab.com.

基因位点

Cytogenetic band: 11p11.2 by HGNC 11p11.2 by Entrez Gene 11p11.2 by Ensembl

MDK Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)

通用注释

亚单元:

Homodimer. Interacts with ALK.

功能:

Developmentally regulated, secreted growth factor homologous to pleiotrophin (PTN), which has heparin binding activity. Binds anaplastic lymphoma kinase (ALK) which induces ALK activation and subsequent phosphorylation of the insulin receptor substrate (IRS1), followed by the activation of mitogen-activated protein kinase (MAPK) and PI3-kinase, and the induction of cell proliferation. Involved in neointima formation after arterial injury, possibly by mediating leukocyte recruitment. Also involved in early fetal adrenal gland development.

亚细胞位置:

Secreted

数据库链接

UniGene:

Hs.82045

SMR:

P21741

STRING:

9606.ENSP00000352852

KEGG:

hsa:4192

MIM:

162096

Pfam:

PF01091

Uniprot:

P21741

相关产品

序列长度:

143

序列:

仅供参考，详细请咨询（services@eiaab.com）

50:

100:

143:

KVRQGTLKKA | RYNAQCQETI | RVTKPCTPKT | KAKAKAKKGK | GKD

3D 模型:

3D 结构:

免疫原序列:

验证序列:

[1].

"COMPARATIVE STUDY BETWEEN DKK-1 AND AFP FOR DIAGNOSING OF HEPATOCELLULAR CARCINOMA AMONG EGYPTIAN PATIENTS"

Mohamed F Z, Barakat L A A, Radwan N H, et al.

From: "EUROPEAN JOURNAL OF PHARMACEUTICAL AND MEDICAL RESEARCH"

[1].

"Midkine gene (MDK), a gene for prenatal differentiation and neuroregulation, maps to band 11p11.2 by fluorescence in situ hybridization."

(PMID: 8406506)

[2].

"Cloning, characterization and developmental regulation of two members of a novel human gene family of neurite outgrowth-promoting proteins."

(PMID: 1768439)

[3].

"Amphiregulin-associated protein: complete amino acid sequence of a protein produced by the 12-0-tetradecanoylphorbol-13-acetate-treated human breast adenocarcinoma cell line MCF-7."

(PMID: 1883381)

[4].

"Midkine positively regulates the proliferation of human gastric cancer cells."

(PMID: 19250738)

[5].

"[Relationship between midkine expression and drug efflux in childhood acute lymphoblastic leukemia cells]."

(PMID: 20030935)

[6].

"The angiogenic factor midkine is regulated by dexamethasone and retinoic acid during alveolarization and in alveolar epithelial cells."

(PMID: 19698107)

[7].

"Midkine prevents ventricular remodeling and improves long-term survival after myocardial infarction."

(PMID: 19060126)

[8].

"Midkine promotes tetraspanin-integrin interaction and induces FAK-Stat1alpha pathway contributing to migration/invasiveness of human head and neck squamous cell carcinoma cells."

(PMID: 18851943)

[9].

"Midkine as a prognostic biomarker in oral squamous cell carcinoma."

(PMID: 18682710)

[10].

"Impact of alcohol abuse on protein expression of midkine and excitatory amino acid transporter 1 in the human prefrontal cortex."

(PMID: 18657127)

[1].

人肝素结合细胞因子(MDK)重组蛋白是否是无菌的？

蛋白试剂瓶和蛋白保存液是经过高压灭菌的，但也不能保证蛋白是完全无菌的。如果要求蛋白是无菌的，可以用0.2微米的滤器对蛋白进行过滤。

[2].

人肝素结合细胞因子(MDK)重组蛋白的保存缓冲液是什么？

纯化后的蛋白保存在PBS（58mM Na2HPO4, 17mM NaH2PO4, 68mM NaCl, pH7.4）里，并往里面加入500mM咪唑和10%甘油。

[3].

怎样确定人肝素结合细胞因子(MDK)重组蛋白的浓度？

蛋白浓度的确定没有一个统一的标准，这主要取决于蛋白的氨基酸序列。伊艾博是根据不同测试的组合来测定蛋白浓度。考马斯亮蓝法、BCA法、氨基酸序列和氨基酸全序列分析法等都用来测定蛋白浓度。

[4].

人肝素结合细胞因子(MDK)重组蛋白蛋白保存条件是怎样的？

蛋白应保存在 -20℃或 -80℃条件下，为了避免反复冻融，可以将蛋白分装成小份保存。

[5].

人肝素结合细胞因子(MDK)重组蛋白是否可以用于活体实验？

重组蛋白没有用于任何的活体实验，因此蛋白的活性和半衰期是不确定的。

[6].

人肝素结合细胞因子(MDK)重组蛋白的保质期是多久？

在适当的保存条件下，从购买之日起蛋白可以稳定保存6-12个月。适当的保存条件是:蛋白保存在-20°C o或 -80℃，保证蛋白的保存浓度高于0.1mg/ml，限制蛋白反复冻融的次数。我们公司常规的质量检测保证所有产品在销售时都有可接受的生物活性。但是我们不能控制终端用户蛋白的保存条件。如果产品在有效期内出现问题，请联系我们的技术支持。

[7].

你们蛋白和抗体的报价是怎么样的？

我们将根据你需要的蛋白和抗体的大小进行报价。

[8].

人肝素结合细胞因子(MDK)重组蛋白是否能够提供蛋白片段？

我们现有的人的蛋白的序列可以有很多。你可以选择你感兴趣的靶向部分，我们将会按您的需求提供蛋白和抗体。

[9].

人肝素结合细胞因子(MDK)重组蛋白的货期或发货时间一般是多长？

具体指标的货期需要确定。最快一周，最长可能一个月。

反馈墙

评论数 : 0

所有用户