Mapk8 (基因名), Mitogen-activated protein kinase 8 (蛋白名), MK08_MOUSE.
Mouse Mapk8/ Mitogen-activated protein kinase 8 Recombinant Protein
Stress-activated protein kinase JNK1, c-Jun N-terminal kinase 1, MAP kinase 8, Jnk1, Prkm8
>90% by SDS-PAGE
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50mM NaH2PO4, 500mM NaCl Buffer with 500mM Imidazole, 10%glycerol(PH8.0)
Store at -20°C. (Avoid repeated freezing and thawing.)
Binds to at least four scaffolding proteins, MAPK8IP1/JIP-1, MAPK8IP2/JIP-2, MAPK8IP3/JIP-3/JSAP1 and SPAG9/MAPK8IP4/JIP-4. These proteins also bind other components of the JNK signaling pathway. Forms a complex with MAPK8IP1 and ARHGEF28. Interacts with TP53 and WWOX. Interacts with JAMP. Interacts with NFATC4. Interacts with MECOM; regulates JNK signaling. Interacts with PIN1; this interaction mediates MAPK8 conformational changes leading to the binding of MAPK8 to its substrates (By similarity). Interacts with HSF1 (via D domain and preferentially with hyperphosphorylated form); this interaction occurs under both normal growth conditions and immediately upon heat shock (By similarity). Interacts (phosphorylated form) with NFE2; the interaction phosphorylates NFE2 in undifferentiated cells.
Serine/threonine-protein kinase involved in various processes such as cell proliferation, differentiation, migration, transformation and programmed cell death. Extracellular stimuli such as proinflammatory cytokines or physical stress stimulate the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. In this cascade, two dual specificity kinases MAP2K4/MKK4 and MAP2K7/MKK7 phosphorylate and activate MAPK8/JNK1. In turn, MAPK8/JNK1 phosphorylates a number of transcription factors, primarily components of AP-1 such as JUN, JDP2 and ATF2 and thus regulates AP-1 transcriptional activity. Phosphorylates the replication licensing factor CDT1, inhibiting the interaction between CDT1 and the histone H4 acetylase HBO1 to replication origins. Loss of this interaction abrogates the acetylation required for replication initiation. Promotes stressed cell apoptosis by phosphorylating key regulatory factors including p53/TP53 and Yes-associates protein YAP1. In T-cells, MAPK8 and MAPK9 are required for polarized differentiation of T-helper cells into Th1 cells. Contributes to the survival of erythroid cells by phosphorylating the antagonist of cell death BAD upon EPO stimulation. Mediates starvation-induced BCL2 phosphorylation, BCL2 dissociation from BECN1, and thus activation of autophagy. Phosphorylates STMN2 and hence regulates microtubule dynamics, controlling neurite elongation in cortical neurons. In the developing brain, through its cytoplasmic activity on STMN2, negatively regulates the rate of exit from multipolar stage and of radial migration from the ventricular zone (By similarity). Phosphorylates several other substrates including heat shock factor protein 4 (HSF4), the deacetylase SIRT1, ELK1, or the E3 ligase ITCH. Phosphorylates the CLOCK-ARNTL/BMAL1 heterodimer and plays a role in the regulation of the circadian clock (PubMed:22441692). Phosphorylates the heat shock transcription factor HSF1, suppressing HSF1-induced transcriptional activity.
MSRSKRDNNF | YSVEIGDSTF | TVLKRYQNLK | PIGSGAQGIV | CAAYDAILER |
NVAIKKLSRP | FQNQTHAKRA | YRELVLMKCV | NHKNIIGLLN | VFTPQKSLEE |
FQDVYIVMEL | MDANLCQVIQ | MELDHERMSY | LLYQMLCGIK | HLHSAGIIHR |
DLKPSNIVVK | SDCTLKILDF | GLARTAGTSF | MMTPYVVTRY | YRAPEVILGM |
GYKENVDLWS | VGCIMGEMVC | HKILFPGRDY | IDQWNKVIEQ | LGTPCPEFMK |
KLQPTVRTYV | ENRPKYAGYS | FEKLFPDVLF | PADSEHNKLK | ASQARDLLSK |
MLVIDASKRI | SVDEALQHPY | INVWYDPSEA | EAPPPKIPDK | QLDEREHTIE |
EWKELIYKEV | MDLEERTKNG | VIRGQPSPLA | QVQQ
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