MAPK3 (基因名), Mitogen-activated protein kinase 3 (蛋白名), MK03_HUMAN.
Human MAPK3/ Mitogen-activated protein kinase 3 Recombinant Protein
ERT2, Extracellular signal-regulated kinase 1, Insulin-stimulated MAP2 kinase, MAP kinase isoform p44, Microtubule-associated protein 2 kinase, p44-ERK1, ERK-1, p44-MAPK, MAP kinase 3, ERK1, PRKM3
>90% by SDS-PAGE
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50mM NaH2PO4, 500mM NaCl Buffer with 500mM Imidazole, 10%glycerol(PH8.0)
Store at -20°C. (Avoid repeated freezing and thawing.)
Binds both upstream activators and downstream substrates in multimolecular complexes. Found in a complex with at least BRAF, HRAS, MAP2K1/MEK1, MAPK3 and RGS14 (By similarity). Binds to HIV-1 Nef through its SH3 domain. This interaction inhibits its tyrosine-kinase activity. Interacts with ADAM15, ARRB2, CANX, DAPK1 (via death domain), HSF4, IER3, MAP2K1/MEK1, MORG1, NISCH, and SGK1. Interacts with PEA15 and MKNK2 (By similarity). MKNK2 isoform 1 binding prevents from dephosphorylation and inactivation (By similarity). Interacts with TPR. Interacts with CDKN2AIP. Interacts with HSF1 (via D domain and preferentially with hyperphosphorylated form); this interaction occurs upon heat shock (PubMed:10747973).
Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK cascade. They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF. Depending on the cellular context, the MAPK/ERK cascade mediates diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. The MAPK/ERK cascade plays also a role in initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors. About 160 substrates have already been discovered for ERKs. Many of these substrates are localized in the nucleus, and seem to participate in the regulation of transcription upon stimulation. However, other substrates are found in the cytosol as well as in other cellular organelles, and those are responsible for processes such as translation, mitosis and apoptosis. Moreover, the MAPK/ERK cascade is also involved in the regulation of the endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC); as well as in the fragmentation of the Golgi apparatus during mitosis. The substrates include transcription factors (such as ATF2, BCL6, ELK1, ERF, FOS, HSF4 or SPZ1), cytoskeletal elements (such as CANX, CTTN, GJA1, MAP2, MAPT, PXN, SORBS3 or STMN1), regulators of apoptosis (such as BAD, BTG2, CASP9, DAPK1, IER3, MCL1 or PPARG), regulators of translation (such as EIF4EBP1) and a variety of other signaling-related molecules (like ARHGEF2, FRS2 or GRB10). Protein kinases (such as RAF1, RPS6KA1/RSK1, RPS6KA3/RSK2, RPS6KA2/RSK3, RPS6KA6/RSK4, SYK, MKNK1/MNK1, MKNK2/MNK2, RPS6KA5/MSK1, RPS6KA4/MSK2, MAPKAPK3 or MAPKAPK5) and phosphatases (such as DUSP1, DUSP4, DUSP6 or DUSP16) are other substrates which enable the propagation the MAPK/ERK signal to additional cytosolic and nuclear targets, thereby extending the specificity of the cascade.
Cytoplasm Nucleus Autophosphorylation at Thr-207 promotes nuclear localization.
MAAAAAQGGG | GGEPRRTEGV | GPGVPGEVEM | VKGQPFDVGP | RYTQLQYIGE |
GAYGMVSSAY | DHVRKTRVAI | KKISPFEHQT | YCQRTLREIQ | ILLRFRHENV |
IGIRDILRAS | TLEAMRDVYI | VQDLMETDLY | KLLKSQQLSN | DHICYFLYQI |
LRGLKYIHSA | NVLHRDLKPS | NLLINTTCDL | KICDFGLARI | ADPEHDHTGF |
LTEYVATRWY | RAPEIMLNSK | GYTKSIDIWS | VGCILAEMLS | NRPIFPGKHY |
LDQLNHILGI | LGSPSQEDLN | CIINMKARNY | LQSLPSKTKV | AWAKLFPKSD |
SKALDLLDRM | LTFNPNKRIT | VEEALAHPYL | EQYYDPTDEP | VAEEPFTFAM |
ELDDLPKERL | KELIFQETAR | FQPGVLEAP
"Molecular cloning, expression, and characterization of the human mitogen-activated protein kinase p44erk1."
"Extracellular signal-regulated kinases in T cells: characterization of human ERK1 and ERK2 cDNAs."
"Collaborator of ARF (CARF) regulates proliferative fate of human cells by dose-dependent regulation of DNA damage signaling."
"ERK2 but not ERK1 induces epithelial-to-mesenchymal transformation via DEF motif-dependent signaling events."
"High-density association study of 383 candidate genes for volumetric BMD at the femoral neck and lumbar spine among older men."
"Genetical genomic determinants of alcohol consumption in rats and humans."
"Tumor suppressor density-enhanced phosphatase-1 (DEP-1) inhibits the RAS pathway by direct dephosphorylation of ERK1/2 kinases."
"Protein kinase SGK1 enhances MEK/ERK complex formation through the phosphorylation of ERK2: implication for the positive regulatory role of SGK1 on the ERK function during liver regeneration."
"The ERK signaling cascade--views from different subcellular compartments."
"A new type of ERK1/2 autophosphorylation causes cardiac hypertrophy."
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