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MIF (基因名), Macrophage migration inhibitory factor (蛋白名), MIF_HUMAN.

产品名称:

Human MIF/ Macrophage migration inhibitory factor Recombinant Protein
巨噬细胞迁移抑制因子

货号:

R0698h

商标:

EIAab^®

监管等级:

RUO

别名:

Glycosylation-inhibiting factor, L-dopachrome isomerase, L-dopachrome tautomerase, Phenylpyruvate tautomerase, GIF, MIF, GLIF, MMIF

序列号:

P14174

来源:

E.coli

种属:

Human

标签:

His

序列:

1-115aa

预估分子量:

12.65 kDa (monomer)

纯度:

Greater than 95% by SDS-PAGE

浓度:

Reconstitution Dependent

形态:

Liquid

内毒素水平:

Please contact protein@eiaab.com The technician for more information.

应用:

存储缓冲液:

50mM NaH2PO4, 500mM NaCl Buffer with 500mM Imidazole, 10%glycerol(PH8.0)

存储:

Store at -20°C. (Avoid repeated freezing and thawing.)

研究领域:

Immunology

规格 & 价格:

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Human MIF Protein

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产品说明书

数据表:

MSDS:

在线询价

数据
文献 ( 1 )
出版物
序列 / 3D
常见问题解答
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R&D 技术数据

The sequence analysis of human MIF protein including hydrophilcity, hydrophobicity and antigenicity.

Recombinant human MIF protein was determined by 12% SDS-PAGE stained with Coomassie Blue under reducing conditions.

基因位点

Cytogenetic band: 22q11.23 by HGNC 22q11.23 by Entrez Gene 22q11.23 by Ensembl

MIF Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)

通用注释

亚单元:

Homotrimer. Interacts with CXCR2 extracellular domain (By similarity). Interacts with the CD74 extracellular domain, COPS5 and BNIPL.

功能:

Pro-inflammatory cytokine. Involved in the innate immune response to bacterial pathogens. The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense. Counteracts the anti-inflammatory activity of glucocorticoids. Has phenylpyruvate tautomerase and dopachrome tautomerase activity (in vitro), but the physiological substrate is not known. It is not clear whether the tautomerase activity has any physiological relevance, and whether it is important for cytokine activity.

亚细胞位置:

Secreted Cytoplasm Does not have a cleavable signal sequence and is secreted via a specialized, non-classical pathway. Secreted by macrophages upon stimulation by bacterial lipopolysaccharide (LPS), or by M.tuberculosis antigens.

数据库链接

UniGene:

Hs.407995

SMR:

P14174

STRING:

9606.ENSP00000215754

KEGG:

hsa:4282

MIM:

153620

Pfam:

PF01187

Uniprot:

P14174

相关产品

Mouse Mif ELISA Kit

Rat Mif ELISA Kit

Bovine MIF ELISA Kit

Chicken MIF ELISA Kit

Pig MIF ELISA Kit

序列长度:

115

序列:

仅供参考，详细请咨询（services@eiaab.com）

50:

100:

116:

YDMNAANVGW | NNSTFA

3D 模型:

3D 结构:

免疫原序列:

验证序列:

[1].

"A genetic role for macrophage migration inhibitory factor (MIF) in Epinephelus awoara infected with Vibrio parahaemolyticus"

Xu X, Sang B, Luo G.

From: European Journal of Inflammation

[1].

"Serum macrophage migration inhibitory factor (MIF) in the intercritical phase of hereditary periodic fevers and its relationship with the MIF-173G/C polymorphism."

(PMID: 17763209)

[2].

"A polymorphism in the macrophage migration inhibitory factor gene is involved in the genetic predisposition of Crohn's disease and associated with cumulative steroid doses."

(PMID: 17591062)

[3].

"A macrophage migration inhibitory factor promoter polymorphism is associated with high-density parasitemia in children with malaria."

(PMID: 16929348)

[4].

"Correlation of rheumatoid arthritis severity with the genetic functional variants and circulating levels of macrophage migration inhibitory factor."

(PMID: 16200611)

[5].

"Coumarin and chromen-4-one analogues as tautomerase inhibitors of macrophage migration inhibitory factor: discovery and X-ray crystallography."

(PMID: 11170644)

[6].

"A novel 5'-flanking region polymorphism of macrophage migration inhibitory factor is associated with systemic-onset juvenile idiopathic arthritis."

(PMID: 11508429)

[7].

"Intracellular action of the cytokine MIF to modulate AP-1 activity and the cell cycle through Jab1."

(PMID: 11089976)

[8].

"Pro-1 of macrophage migration inhibitory factor functions as a catalytic base in the phenylpyruvate tautomerase activity."

(PMID: 10353846)

[9].

"Molecular cloning and functional expression of a cDNA encoding glycosylation-inhibiting factor."

(PMID: 8234256)

[10].

"Molecular cloning of a cDNA encoding a human macrophage migration inhibitory factor."

(PMID: 2552447)

[1].

人巨噬细胞迁移抑制因子(MIF)重组蛋白是否是无菌的？

蛋白试剂瓶和蛋白保存液是经过高压灭菌的，但也不能保证蛋白是完全无菌的。如果要求蛋白是无菌的，可以用0.2微米的滤器对蛋白进行过滤。

[2].

人巨噬细胞迁移抑制因子(MIF)重组蛋白的保存缓冲液是什么？

纯化后的蛋白保存在PBS（58mM Na2HPO4, 17mM NaH2PO4, 68mM NaCl, pH7.4）里，并往里面加入500mM咪唑和10%甘油。

[3].

怎样确定人巨噬细胞迁移抑制因子(MIF)重组蛋白的浓度？

蛋白浓度的确定没有一个统一的标准，这主要取决于蛋白的氨基酸序列。伊艾博是根据不同测试的组合来测定蛋白浓度。考马斯亮蓝法、BCA法、氨基酸序列和氨基酸全序列分析法等都用来测定蛋白浓度。

[4].

人巨噬细胞迁移抑制因子(MIF)重组蛋白蛋白保存条件是怎样的？

蛋白应保存在 -20℃或 -80℃条件下，为了避免反复冻融，可以将蛋白分装成小份保存。

[5].

人巨噬细胞迁移抑制因子(MIF)重组蛋白是否可以用于活体实验？

重组蛋白没有用于任何的活体实验，因此蛋白的活性和半衰期是不确定的。

[6].

人巨噬细胞迁移抑制因子(MIF)重组蛋白的保质期是多久？

在适当的保存条件下，从购买之日起蛋白可以稳定保存6-12个月。适当的保存条件是:蛋白保存在-20°C o或 -80℃，保证蛋白的保存浓度高于0.1mg/ml，限制蛋白反复冻融的次数。我们公司常规的质量检测保证所有产品在销售时都有可接受的生物活性。但是我们不能控制终端用户蛋白的保存条件。如果产品在有效期内出现问题，请联系我们的技术支持。

[7].

你们蛋白和抗体的报价是怎么样的？

我们将根据你需要的蛋白和抗体的大小进行报价。

[8].

人巨噬细胞迁移抑制因子(MIF)重组蛋白是否能够提供蛋白片段？

我们现有的人的蛋白的序列可以有很多。你可以选择你感兴趣的靶向部分，我们将会按您的需求提供蛋白和抗体。

[9].

人巨噬细胞迁移抑制因子(MIF)重组蛋白的货期或发货时间一般是多长？

具体指标的货期需要确定。最快一周，最长可能一个月。

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