首页 > 重组蛋白 > Human CHCHD4 Recombinant Protein

CHCHD4 (基因名), Mitochondrial intermembrane space import and assembly protein 40 (蛋白名), MIA40_HUMAN.

产品名称:

Human CHCHD4/ Mitochondrial intermembrane space import and assembly protein 40 Recombinant Protein

货号:

商标:

EIAab^®

监管等级:

RUO

别名:

Coiled-coil-helix-coiled-coil-helix domain-containing protein 4, MIA40

序列号:

Q8N4Q1

来源:

E.coli

种属:

Human

标签:

His

纯度:

>90% by SDS-PAGE

浓度:

Reconstitution Dependent

形态:

Liquid

内毒素水平:

Please contact protein@eiaab.com The technician for more information.

应用:

存储缓冲液:

50mM NaH2PO4, 500mM NaCl Buffer with 500mM Imidazole, 10%glycerol(PH8.0)

存储:

Store at -20°C. (Avoid repeated freezing and thawing.)

研究领域:

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Human CHCHD4 Protein

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产品说明书

说明书:

MSDS:

在线询价

数据
文献
出版物
序列 / 3D
常见问题解答
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基因位点

Cytogenetic band: 3p25.1 by HGNC 3p25.1 by Entrez Gene 3p25.1 by Ensembl

CHCHD4 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)

通用注释

亚单元:

Monomer (PubMed:19182799). Can form homooligomers (PubMed:16185709). Interacts with GFER and forms transient disulfide bonds with GFER (PubMed:23186364). Interacts with MICU1 (PubMed:26387864).

功能:

Functions as chaperone and catalyzes the formation of disulfide bonds in substrate proteins, such as COX17 or MICU1 (PubMed:16185709, PubMed:26387864, PubMed:19182799, PubMed:21059946, PubMed:23186364). Required for the import and folding of small cysteine-containing proteins (small Tim) in the mitochondrial intermembrane space (IMS) (PubMed:16185709, PubMed:19182799, PubMed:21059946). Precursor proteins to be imported into the IMS are translocated in their reduced form into the mitochondria (PubMed:16185709, PubMed:19182799, PubMed:21059946). The oxidized form of CHCHD4/MIA40 forms a transient intermolecular disulfide bridge with the reduced precursor protein, resulting in oxidation of the precursor protein that now contains an intramolecular disulfide bond and is able to undergo folding in the IMS (PubMed:16185709, PubMed:19182799, PubMed:21059946). Reduced CHCHD4/MIA40 is then reoxidized by GFER/ERV1 via a disulfide relay system (PubMed:23186364). Mediates formation of disulfide bond in MICU1 in the IMS, promoting formation of the MICU1-MICU2 heterodimer that regulates mitochondrial calcium uptake (PubMed:26387864).

亚细胞位置:

Mitochondrion intermembrane space

数据库链接

UniGene:

Hs.517815

SMR:

Q8N4Q1

STRING:

9606.ENSP00000295767

KEGG:

hsa:131474

MIM:

611077

Pfam:

PF06747

Uniprot:

Q8N4Q1

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序列长度:

142

序列:

仅供参考，详细请咨询（services@eiaab.com）

50:

100:

142:

YPDLYPQEDE | DEEEEREKKP | AEQAEETAPI | EATATKEEEG | SS

3D 模型:

3D 结构:

免疫原序列:

验证序列:

该产品尚未在任何出版物中被引用。

[1].

"The Ca(2+)-Dependent Release of the Mia40-Induced MICU1-MICU2 Dimer from MCU Regulates Mitochondrial Ca(2+) Uptake."

(PMID: 26387864)

[2].

"Protein import and oxidative folding in the mitochondrial intermembrane space of intact mammalian cells."

(PMID: 23676665)

[3].

"Disulfide bond formation: sulfhydryl oxidase ALR controls mitochondrial biogenesis of human MIA40."

(PMID: 23186364)

[4].

"Human CHCHD4 mitochondrial proteins regulate cellular oxygen consumption rate and metabolism and provide a critical role in hypoxia signaling and tumor progression."

(PMID: 22214851)

[5].

"Genetic variants in nuclear-encoded mitochondrial genes influence AIDS progression."

(PMID: 20877624)

[6].

"Molecular chaperone function of Mia40 triggers consecutive induced folding steps of the substrate in mitochondrial protein import."

(PMID: 21059946)

[7].

"MIA40 is an oxidoreductase that catalyzes oxidative protein folding in mitochondria."

(PMID: 19182799)

[8].

"Functional and mutational characterization of human MIA40 acting during import into the mitochondrial intermembrane space."

(PMID: 16185709)

[9].

"Complete sequencing and characterization of 21,243 full-length human cDNAs."

(PMID: 14702039)

[10].

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."

(PMID: 15489334)

[1].

人CHCHD4重组蛋白是否是无菌的？

蛋白试剂瓶和蛋白保存液是经过高压灭菌的，但也不能保证蛋白是完全无菌的。如果要求蛋白是无菌的，可以用0.2微米的滤器对蛋白进行过滤。

[2].

人CHCHD4重组蛋白的保存缓冲液是什么？

纯化后的蛋白保存在PBS（58mM Na2HPO4, 17mM NaH2PO4, 68mM NaCl, pH7.4）里，并往里面加入500mM咪唑和10%甘油。

[3].

怎样确定人CHCHD4重组蛋白的浓度？

蛋白浓度的确定没有一个统一的标准，这主要取决于蛋白的氨基酸序列。伊艾博是根据不同测试的组合来测定蛋白浓度。考马斯亮蓝法、BCA法、氨基酸序列和氨基酸全序列分析法等都用来测定蛋白浓度。

[4].

人CHCHD4重组蛋白蛋白保存条件是怎样的？

蛋白应保存在 -20℃或 -80℃条件下，为了避免反复冻融，可以将蛋白分装成小份保存。

[5].

人CHCHD4重组蛋白是否可以用于活体实验？

重组蛋白没有用于任何的活体实验，因此蛋白的活性和半衰期是不确定的。

[6].

人CHCHD4重组蛋白的保质期是多久？

在适当的保存条件下，从购买之日起蛋白可以稳定保存6-12个月。适当的保存条件是:蛋白保存在-20°C o或 -80℃，保证蛋白的保存浓度高于0.1mg/ml，限制蛋白反复冻融的次数。我们公司常规的质量检测保证所有产品在销售时都有可接受的生物活性。但是我们不能控制终端用户蛋白的保存条件。如果产品在有效期内出现问题，请联系我们的技术支持。

[7].

你们蛋白和抗体的报价是怎么样的？

我们将根据你需要的蛋白和抗体的大小进行报价。

[8].

人CHCHD4重组蛋白是否能够提供蛋白片段？

我们现有的人的蛋白的序列可以有很多。你可以选择你感兴趣的靶向部分，我们将会按您的需求提供蛋白和抗体。

[9].

人CHCHD4重组蛋白的货期或发货时间一般是多长？

具体指标的货期需要确定。最快一周，最长可能一个月。

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