首页 > 重组蛋白 > Human MFN1 Recombinant Protein

MFN1 (基因名), Mitofusin-1 (蛋白名), MFN1_HUMAN.

产品名称:

Human MFN1/ Mitofusin-1 Recombinant Protein
Mitofusin-1

货号:

R15253h

商标:

EIAab^®

监管等级:

RUO

别名:

Fzo homolog, Transmembrane GTPase MFN1

序列号:

Q8IWA4

来源:

E.coli

种属:

Human

标签:

His

序列:

2-77aa

预估分子量:

8.36 kDa (monomer)

纯度:

Greater than 95% by SDS-PAGE

浓度:

Reconstitution Dependent

形态:

Liquid

内毒素水平:

Please contact protein@eiaab.com The technician for more information.

应用:

存储缓冲液:

50mM NaH2PO4, 500mM NaCl Buffer with 500mM Imidazole, 10%glycerol(PH8.0)

存储:

Store at -20°C. (Avoid repeated freezing and thawing.)

研究领域:

Neurosciences

规格 & 价格:

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Human MFN1 Protein

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产品说明书

说明书:

MSDS:

在线询价

数据
文献
出版物
序列 / 3D
常见问题解答
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R&D 技术数据

The PCR product of human MFN1 gene was determined by 1% Agarose stained with EB.

Recombinant human MFN1 protein was determined by 12% SDS-PAGE stained with Coomassie Blue under reducing conditions.

基因位点

Cytogenetic band: 3q26.33 by HGNC 3q26.33 by Entrez Gene 3q26.33 by Ensembl

MFN1 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)

通用注释

亚单元:

Homodimer, also in the absence of bound GTP (PubMed:27920125, PubMed:28114303). Forms higher oligomers in the presence of a transition state GTP analog (PubMed:28114303). Forms homomultimers and heteromultimers with MFN2 (By similarity). Oligomerization is essential for mitochondrion fusion (PubMed:27920125, PubMed:28114303). Component of a high molecular weight multiprotein complex (PubMed:12759376). Interacts with VAT1.

功能:

Mitochondrial outer membrane GTPase that mediates mitochondrial clustering and fusion (PubMed:12475957, PubMed:12759376, PubMed:27920125, PubMed:28114303). Membrane clustering requires GTPase activity (PubMed:27920125). It may involve a major rearrangement of the coiled coil domains (PubMed:27920125, PubMed:28114303). Mitochondria are highly dynamic organelles, and their morphology is determined by the equilibrium between mitochondrial fusion and fission events (PubMed:12475957, PubMed:12759376). Overexpression induces the formation of mitochondrial networks (in vitro) (PubMed:12759376). Has low GTPase activity (PubMed:27920125, PubMed:28114303).

亚细胞位置:

Isoform 2 Cytoplasm

数据库链接

UniGene:

Hs.478383

SMR:

Q8IWA4

STRING:

9606.ENSP00000263969

KEGG:

hsa:55669

MIM:

608506

Pfam:

PF00350

Uniprot:

Q8IWA4

相关产品

序列长度:

741

序列:

仅供参考，详细请咨询（services@eiaab.com）

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741:

EIDQLEKIQN | NSKLLRNKAV | QLENELENFT | KQFLPSSNEE | S

3D 模型:

3D 结构:

免疫原序列:

验证序列:

该产品尚未在任何出版物中被引用。

[1].

"Control of mitochondrial morphology by a human mitofusin."

(PMID: 11181170)

[2].

"Loss of MARCH5 mitochondrial E3 ubiquitin ligase induces cellular senescence through dynamin-related protein 1 and mitofusin 1."

(PMID: 20103533)

[3].

"Evaluation of nine candidate genes in patients with normal tension glaucoma: a case control study."

(PMID: 19754948)

[4].

"Mitofusin-1 protein is a generally expressed mediator of mitochondrial fusion in mammalian cells."

(PMID: 12759376)

[5].

"Mitochondrial fusion in human cells is efficient, requires the inner membrane potential, and is mediated by mitofusins."

(PMID: 12475957)

[6].

"MFN1 structures reveal nucleotide-triggered dimerization critical for mitochondrial fusion."

(PMID: 28114303)

[7].

"Structures of human mitofusin 1 provide insight into mitochondrial tethering."

(PMID: 27920125)

[8].

"Genetic variants in nuclear-encoded mitochondrial genes influence AIDS progression."

(PMID: 20877624)

[9].

"Mitofusins 1/2 and ERRalpha expression are increased in human skeletal muscle after physical exercise."

(PMID: 15961417)

[10].

"Complete sequencing and characterization of 21,243 full-length human cDNAs."

(PMID: 14702039)

[1].

人Mitofusin-1(MFN1)重组蛋白是否是无菌的？

蛋白试剂瓶和蛋白保存液是经过高压灭菌的，但也不能保证蛋白是完全无菌的。如果要求蛋白是无菌的，可以用0.2微米的滤器对蛋白进行过滤。

[2].

人Mitofusin-1(MFN1)重组蛋白的保存缓冲液是什么？

纯化后的蛋白保存在PBS（58mM Na2HPO4, 17mM NaH2PO4, 68mM NaCl, pH7.4）里，并往里面加入500mM咪唑和10%甘油。

[3].

怎样确定人Mitofusin-1(MFN1)重组蛋白的浓度？

蛋白浓度的确定没有一个统一的标准，这主要取决于蛋白的氨基酸序列。伊艾博是根据不同测试的组合来测定蛋白浓度。考马斯亮蓝法、BCA法、氨基酸序列和氨基酸全序列分析法等都用来测定蛋白浓度。

[4].

人Mitofusin-1(MFN1)重组蛋白蛋白保存条件是怎样的？

蛋白应保存在 -20℃或 -80℃条件下，为了避免反复冻融，可以将蛋白分装成小份保存。

[5].

人Mitofusin-1(MFN1)重组蛋白是否可以用于活体实验？

重组蛋白没有用于任何的活体实验，因此蛋白的活性和半衰期是不确定的。

[6].

人Mitofusin-1(MFN1)重组蛋白的保质期是多久？

在适当的保存条件下，从购买之日起蛋白可以稳定保存6-12个月。适当的保存条件是:蛋白保存在-20°C o或 -80℃，保证蛋白的保存浓度高于0.1mg/ml，限制蛋白反复冻融的次数。我们公司常规的质量检测保证所有产品在销售时都有可接受的生物活性。但是我们不能控制终端用户蛋白的保存条件。如果产品在有效期内出现问题，请联系我们的技术支持。

[7].

你们蛋白和抗体的报价是怎么样的？

我们将根据你需要的蛋白和抗体的大小进行报价。

[8].

人Mitofusin-1(MFN1)重组蛋白是否能够提供蛋白片段？

我们现有的人的蛋白的序列可以有很多。你可以选择你感兴趣的靶向部分，我们将会按您的需求提供蛋白和抗体。

[9].

人Mitofusin-1(MFN1)重组蛋白的货期或发货时间一般是多长？

具体指标的货期需要确定。最快一周，最长可能一个月。

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