EIF4EBP2 (基因名), Eukaryotic translation initiation factor 4E-binding protein 2 (蛋白名), 4EBP2_HUMAN.
产品名称:
Human EIF4EBP2/ Eukaryotic translation initiation factor 4E-binding protein 2 Recombinant Protein
真核翻译起始因子4e结合蛋白2
货号:
R2694h
商标:
EIAab®
监管等级:
别名:
4E-BP2
序列号:
Q13542
来源:
E.coli
种属:
Human
标签:
His
纯度:
>90% by SDS-PAGE
浓度:
Reconstitution Dependent
形态:
Liquid
内毒素水平:
Please contact protein@eiaab.com The technician for more information.
应用:
存储缓冲液:
50mM NaH2PO4, 500mM NaCl Buffer with 500mM Imidazole, 10%glycerol(PH8.0)
存储:
Store at -20°C. (Avoid repeated freezing and thawing.)
研究领域:
-
R&D 技术数据
更多信息，请参阅手册，或联系我们的技术支持: tech@eiaab.com.
基因位点
EIF4EBP2 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
通用注释
亚单元:
Hypophosphorylated EIF4EBP2 interacts with EIF4E; phosphorylation of EIF4EBP2 by mTORC1 causes dissociation of the complex allowing EIF4G1/EIF4G3 to bind and consequent initiation of translation (PubMed:7935836, PubMed:24207126, PubMed:21661078, PubMed:25533957). Interacts with RPTOR; promoting phosphorylation by mTORC1 (By similarity). Interacts with PCMT1; required to prevent isoaspartate accumulation and convert isoaspartate to Asp.
功能:
Repressor of translation initiation involved in synaptic plasticity, learning and memory formation (By similarity). Regulates EIF4E activity by preventing its assembly into the eIF4F complex: hypophosphorylated form of EIF4EBP2 competes with EIF4G1/EIF4G3 and strongly binds to EIF4E, leading to repress translation. In contrast, hyperphosphorylated form dissociates from EIF4E, allowing interaction between EIF4G1/EIF4G3 and EIF4E, leading to initiation of translation (PubMed:25533957). EIF4EBP2 is enriched in brain and acts as a regulator of synapse activity and neuronal stem cell renewal via its ability to repress translation initiation (By similarity). Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways.
亚细胞位置:
N/A
数据库链接
验证序列:
该产品尚未在任何出版物中被引用。
[1].
"Tissue distribution, genomic structure, and chromosome mapping of mouse and human eukaryotic initiation factor 4E-binding proteins 1 and 2."
[2].
"Insulin-dependent stimulation of protein synthesis by phosphorylation of a regulator of 5'-cap function."
[3].
"Folding of an intrinsically disordered protein by phosphorylation as a regulatory switch."
[4].
"Interaction of the eukaryotic initiation factor 4E with 4E-BP2 at a dynamic bipartite interface."
[5].
"Structural scaffold for eIF4E binding selectivity of 4E-BP isoforms: crystal structure of eIF4E binding region of 4E-BP2 and its comparison with that of 4E-BP1."
[6].
"Postnatal deamidation of 4E-BP2 in brain enhances its association with raptor and alters kinetics of excitatory synaptic transmission."
[7].
"Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study."
[8].
"Evaluation of candidate stromal epithelial cross-talk genes identifies association between risk of serous ovarian cancer and TERT, a cancer susceptibility "hot-spot"."
[9].
"Binding preference of eIF4E for 4E-binding protein isoform and function of eIF4E N-terminal flexible region for interaction, studied by SPR analysis."
[10].
"A scan of chromosome 10 identifies a novel locus showing strong association with late-onset Alzheimer disease."
[1].
人真核翻译起始因子4e结合蛋白2(EIF4EBP2)重组蛋白是否是无菌的？
蛋白试剂瓶和蛋白保存液是经过高压灭菌的，但也不能保证蛋白是完全无菌的。如果要求蛋白是无菌的，可以用0.2微米的滤器对蛋白进行过滤。
[2].
人真核翻译起始因子4e结合蛋白2(EIF4EBP2)重组蛋白的保存缓冲液是什么？
纯化后的蛋白保存在PBS（58mM Na2HPO4, 17mM NaH2PO4, 68mM NaCl, pH7.4）里，并往里面加入500mM咪唑和10%甘油。
[3].
怎样确定人真核翻译起始因子4e结合蛋白2(EIF4EBP2)重组蛋白的浓度？
蛋白浓度的确定没有一个统一的标准，这主要取决于蛋白的氨基酸序列。伊艾博是根据不同测试的组合来测定蛋白浓度。考马斯亮蓝法、BCA法、氨基酸序列和氨基酸全序列分析法等都用来测定蛋白浓度。
[4].
人真核翻译起始因子4e结合蛋白2(EIF4EBP2)重组蛋白蛋白保存条件是怎样的？
蛋白应保存在 -20℃或 -80℃条件下，为了避免反复冻融，可以将蛋白分装成小份保存。
[5].
人真核翻译起始因子4e结合蛋白2(EIF4EBP2)重组蛋白是否可以用于活体实验？
重组蛋白没有用于任何的活体实验，因此蛋白的活性和半衰期是不确定的。
[6].
人真核翻译起始因子4e结合蛋白2(EIF4EBP2)重组蛋白的保质期是多久？
在适当的保存条件下，从购买之日起蛋白可以稳定保存6-12个月。适当的保存条件是:蛋白保存在-20°C o或 -80℃，保证蛋白的保存浓度高于0.1mg/ml，限制蛋白反复冻融的次数。我们公司常规的质量检测保证所有产品在销售时都有可接受的生物活性。但是我们不能控制终端用户蛋白的保存条件。如果产品在有效期内出现问题，请联系我们的技术支持。
[7].
你们蛋白和抗体的报价是怎么样的？
我们将根据你需要的蛋白和抗体的大小进行报价。
[8].
人真核翻译起始因子4e结合蛋白2(EIF4EBP2)重组蛋白是否能够提供蛋白片段？
我们现有的人的蛋白的序列可以有很多。你可以选择你感兴趣的靶向部分，我们将会按您的需求提供蛋白和抗体。
[9].
人真核翻译起始因子4e结合蛋白2(EIF4EBP2)重组蛋白的货期或发货时间一般是多长？
具体指标的货期需要确定。最快一周，最长可能一个月。
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