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Nos2 (基因名), Nitric oxide synthase, inducible (蛋白名), nos2_rat.

产品名称:

Rat Nos2/ Nitric oxide synthase, inducible ELISA Kit
诱导型一氧化氮合酶

货号:

E0837r

商标:

EIAab^®

监管等级:

RUO

别名:

Inducible NO synthase, NOS type II, Peptidyl-cysteine S-nitrosylase NOS2, Inducible NOS

检测方法:

ELISA

实验类型:

Sandwich

检测范围:

0.156-10ng/mL

灵敏度:

0.08ng/mL

特异性:

Natural and recombinant rat Nitric oxide synthase, inducible

样品类型:

Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids

样品数据:

实验步骤:

研究领域:

规格 & 价格:

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Rat Nos2 ELISA Kit

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产品说明书

数据表:

说明书:

MSDS:

在线询价

数据
文献 ( 21 )
序列 / 3D
常见问题解答
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精密度

批内差：已知浓度的3个样本在一个板子内重复检测20次，以评估批内精密度。
批内 CV： ≤4.7%
批间差：已知浓度的3个样本在不同的板子上重复测定5次，以评估测定批间精密度。
批间 CV： ≤7.1%

回收率

回收率：低、中和高浓度的分析物被掺入到血清或者血浆样本中，进行回收实验测定。

Sample Type	Average(%)	Recovery Range(%)
Serum	103	97-109
Plasma	105	99-111

线性

线性：给定样本通过梯度稀释，每次稀释的测量值与理论值的比值。

Sample	1：2	1:4	1:8	1:16
serum(n=5)	103-112%	102-110%	97-108%	91-101%
EDTA plasma(n=5)	102-111%	107-116%	105-114%	111-119%
heparin plasma(n=5)	110-120%	108-120%	97-108%	104-114%

通用注释

亚单元:

Homodimer. Binds SLC9A3R1 (By similarity). Interacts with GAPDH. Interacts with S100A8 and S100A9 to form the iNOS-S100A8/9 transnitrosylase complex.

功能:

Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such PTGS2/COX2. As component of the iNOS-S100A8/9 transnitrosylase complex involved in the selective inflammatory stimulus-dependent S-nitrosylation of GAPDH implicated in regulation of the GAIT complex activity and probably multiple targets including ANXA5, EZR, MSN and VIM. Involved in inflammation, enhances the synthesis of proinflammatory mediators such as IL6 and IL8.

亚细胞位置:

N/A

数据库链接

UniGene:

Rn.10400

STRING:

10116.ENSRNOP00000067662

KEGG:

rno:24599

Pfam:

PF00667

Uniprot:

Q06518

相关产品

Human NOS2 ELISA Kit

Mouse Nos2 ELISA Kit

Bovine NOS2 ELISA Kit

Chicken NOS2 ELISA Kit

Pig NOS2 ELISA Kit

序列长度:

1147

序列:

仅供参考，详细请咨询（services@eiaab.com）

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1147:

NLSEEQVEDY | FFQLKSQKRY | HEDIFGAVFS | YGAKKGNTLE | EPKGTRL

3D 模型:

3D 结构:

免疫原序列:

验证序列:

[1].

"Synergistic effect of cranberry extract and losartan against aluminium chloride-induced hepatorenal damage associated cardiomyopathy in rats"

S M Galal, H F Hasan, M K Abdel-Rafei, et al.

From: Archives of Physiology and Biochemistry

[2].

"Montelukast abrogates prednisolone-induced hepatic injury in rats: Modulation of mitochondrial dysfunction, oxidative/nitrosative stress, and apoptosis"

I I Hegab, H E El-Horany, M M. Elbatsh, et al.

From: Journal of Biochemical and Molecular Toxicology

[3].

"Beta-caryophyllene protects against diet-induced dyslipidemia and vascular inflammation in rats: Involvement of CB2 and PPAR-γ receptors"

D A. Youssef, H M. El-Fayoumi, M F. Mahmoud.

From: Chemico-Biological Interactions

[4].

"The anti-Alzheimer effect of telmisartan in a hyperglycemic ovariectomized rat model; role of central angiotensin and estrogen receptors"

A M. Abo-Youssef, W A. Khallaf, M M. Khattab, et al.

From: Food and Chemical Toxicology

[5].

"Bradykinin‐potentiating factor isolated from Leiurus quinquestriatus scorpion venom alleviates cardiomyopathy in irradiated rats via remodelling of the RAAS pathway"

H F Hasan, R R. Radwan, S M Galal.

From: Clinical and Experimental Pharmacology and Physiology

[6].

"Mechanistic exploration of the activities of poly(lactic-co-glycolic acid)-loaded nanoparticles of betulinic acid against hepatocellular carcinoma at cellular and molecular levels"

P Kumar, A K Gautam, U Kumar, et al.

From: Archives of Physiology and Biochemistry

[7].

"Assessment of hyaluronic acid-modified imatinib mesylate cubosomes through CD44 targeted drug delivery in NDEA-induced hepatic carcinoma"

R Nisha, P Kumar, U Kumar, et al.

From: International Journal of Pharmaceutics

[8].

"Protective effect of l-ascorbic acid on nickel induced pulmonary nitrosative stress in male albino rats"

SH Hattiwale, S Saha, SM Yendigeri, et al.

From: BioMetalsBioMetals

[9].

"Temporal determination of lung NO system and COX-2 upregulation following ischemia-reperfusion injury"

D Oleg, E Amar, S Shapira,et al.

From: Experimental lung research

[10].

"Neuroprotective effects of vildagliptin in rat rotenone Parkinson’s disease model: role of RAGE-NFκB and Nrf2-antioxidant signaling ..."

RM Abdelsalam, MM Safar

From: Journal of Neurochemistry

[11].

"Molecular mechanisms of the protective role of wheat germ oil against cyclosporin A-induced hepatotoxicity in rats"

ES Akool

From: Pharmaceutical Biology

[12].

"Protective effects of rosuvastatin in a rat model of lung contusion: Stimulation of the cyclooxygenase 2–prostaglandin E-2 pathway"

O Dolkart, E Amar, S Shapira, et al.

From: Surgery

[13].

"Molecular Mechanisms of the Modulatory Effect of Vitamin E on Tacrolimus (FK506)-Induced Renal Injury in Rats"

H Afify, AAH Abdel-Aziz, MN El-Din, et al.

From: British Journal of Pharmaceutical Research

[14].

"Comparative pharmaceutical study on colon targeted micro-particles of celecoxib: in-vitro–in-vivo evaluation"

Bazan L, Bendas E R, El Gazayerly O N, et al.

From: Drug delivery

[15].

"Effect of Pneumoperitoneum on Oxidative Stress and Inflammation via the Arginase Pathway in Rats"

Shin S, Na S, Kim O S, et al.

From: Yonsei medical journal

[16].

"Mangiferin mitigates gastric ulcer in ischemia/reperfused rats: involvement of PPAR-γ, NF-κB and Nrf2/HO-1 signaling pathways"

Mahmoud-Awny M, Attia A S, Abd-Ellah M F, et al.

From: PloS one

[17].

"The anti-osteoporotic and anti-atherogenic effects of alendronate and simvastatin in ovariectomized rats fed high fat diet: A comparative study of com"

MT Mohamed, S A. Abuelezz, SS Atalla, et al.

From: Biomedicine & Pharmacotherapy

[18].

"Protective effect of cardamonin against acetic acid-induced ulcerative colitis in rats"

AA Ali, ENAA Haleem, SA Khaleel, et al.

From: Pharmacological Reports

[19].

"Neuroprotective Effect of Nanodiamond in Alzheimer’s Disease Rat Model: a Pivotal Role for Modulating NF-κB and STAT3 Signaling"

Shawqi H. A, Ezzeldin S. E, Marwa M. S, et al.

From: Molecular Neurobiology

[20].

"Beta-caryophyllene alleviates diet-induced neurobehavioral changes in rats: The role of CB2 and PPAR-γ receptors"

D A. Youssef, H M. El-Fayoumi, M F. Mahmoud.

From: Biomedicine & Pharmacotherapy

[21].

"Perindopril ameliorates experimental Alzheimer’s disease progression: role of amyloid β degradation, central estrogen receptor and hyperlipidemic-lipid raft signaling"

B A. S. Messiha, M R. A. Ali, M M. Khattab, et al.

From: Inflammopharmacology

[1].

大鼠诱导型一氧化氮合酶(Nos2)ELISA试剂盒可以做多少个样本？

大鼠诱导型一氧化氮合酶(Nos2)ELISA试剂盒分为2种规格，96孔和48孔。96孔的试剂盒，标曲和样本都做复孔的话，可以检测40个样本。96孔的试剂盒，标曲和样本都不做复孔的话，可以检测88个样本。

[2].

大鼠诱导型一氧化氮合酶(Nos2)ELISA试剂盒使用视频？

大鼠诱导型一氧化氮合酶(Nos2)ELISA试剂盒实验操作视频在以下网址中，对每一步的实验步骤都做了演示，方便实验员能更好地理解ELISA实验的过程。
https://www.eiaab.com.cn/lesson-tech/805.html

[3].

大鼠诱导型一氧化氮合酶(Nos2)ELISA试剂盒是放在-20℃冰箱保存吗？

EIAab的大鼠诱导型一氧化氮合酶(Nos2)ELISA试剂盒，洗涤液、底物、终止液保存于4℃，其余试剂-20℃冰箱保存。

[4].

大鼠诱导型一氧化氮合酶(Nos2)ELISA试剂盒原理？

双抗体夹心法：用纯化的抗体包被微孔板，制成固相抗体，往包被有固相抗体的微孔中依次加入标准品或受检样本、生物素化抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。用酶标仪在450nm波长下测定吸光度(OD值）,计算样本浓度。
竞争法：用纯化的抗体包被微孔板，制成固相抗体，往包被有固相抗体的微孔中依次加入标准品或受检样本和生物素标记的目标分析物，受检标本中抗原与生物素标记抗原竞争结合有限的抗体。再加入HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。用酶标仪在450nm波长下测定吸光度(OD值）,计算样本浓度。

[5].

大鼠诱导型一氧化氮合酶(Nos2)ELISA试剂盒中需要使用的样品量是多少？

夹心法100μL/孔，竞争法50μL/孔。如样本浓度过高时，应对样本进行稀释，以使稀释后的样本符合试剂盒的检测范围，计算时再乘以相应的稀释倍数。

[6].

如何分析大鼠诱导型一氧化氮合酶(Nos2)ELISA试剂盒数据？

建议标准曲线，并计算样本浓度。对于elisa的曲线拟合，一般建议采用4参数曲线拟合，4参数曲线拟合通常更适合免疫分析。推荐使用专业软件进行曲线拟合，例如curve expert 1.3。根据样本的OD值由标曲查出相应的浓度，再乘以稀释倍数；或用标准物的浓度与OD值计算出标曲的回归方程式，将样本的OD值代入方程式，计算出样本浓度，再乘以稀释倍数，即为样本的实际浓度。以下链接是curve expert 1.3软件拟合曲线的方法。
https://www.eiaab.com.cn/news/502/

[7].

大鼠诱导型一氧化氮合酶(Nos2)ELISA试剂盒中是否包含人和动物的副产物，是否包含感染的或者传染性原料如HIV等？

除了抗体和稀释液中的BSA，不含其它人和动物的副产物，也不含感染材料。

[8].

收集大鼠诱导型一氧化氮合酶(Nos2)ELISA试剂盒血浆样本，用什么作为抗凝剂？

一般建议用EDTA和肝素作为抗凝剂。

[9].

大鼠诱导型一氧化氮合酶(Nos2)ELISA试剂盒酶标板可以拆成几部分？拆的时候是否需要避光，无菌？

大鼠诱导型一氧化氮合酶(Nos2)ELISA试剂盒酶标板是8×12孔条，可拆卸，板子可以拆成12条，注意避免孔污染，不需要避光和无菌。暂时不用的板子，放回原来装的袋子里，密封保存。

[10].

大鼠诱导型一氧化氮合酶(Nos2)ELISA试剂盒样本如何保存？

尽量检测新鲜样本。若无新鲜样本，则4℃保存1周，-20℃保存1个月，-80℃保存2个月。

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Rat Nos2/ Nitric oxide synthase, inducible ELISA Kit 诱导型一氧化氮合酶

E0837r

Inducible NO synthase, NOS type II, Peptidyl-cysteine S-nitrosylase NOS2, Inducible NOS

Rat Nos2/ Nitric oxide synthase, inducible ELISA Kit
诱导型一氧化氮合酶