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Klf5 (GeneName), Krueppel-like factor 5 (ProteinName), klf5_mouse.
Product Name:

Mouse Klf5/ Krueppel-like factor 5 ELISA Kit

 
Cat.#:

E1047m

Brand:
EIAab®
Regulatory Status:
Alternative:

Basic transcription element-binding protein 2,BTE-binding protein 2,Intestinal-enriched krueppel-like factor,Transcription factor BTEB2,Bteb2,Iklf

Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
0.156-10ng/mL
Sensitivity:
0.078ng/mL
Specificity:
Natural and recombinant mouse Krueppel-like factor 5
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Research Area:
Cancer
Product Overview:
E1047m is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A Disintegrin and Metalloprotease 30 (Krueppel-like factor 5) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Krueppel-like factor 5. The ELISA analytical biochemical technique of the E1047m kit is based on Krueppel-like factor 5 antibody-Krueppel-like factor 5 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Krueppel-like factor 5 antigen targets in samples. The ELISA Kit is designed to detect native, recombinant, Krueppel-like factor 5. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Mouse Klf5 ELISA Kit
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Product Datasheets
Datasheet:
Instruction:
MSDS:


Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤7.6%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤9.5%

Recovery
Recovery was determined by spiking various levels of Krueppel-like factor 5 into serum and plasma.

Sample Type

Average(%)

Recovery Range(%)

Serum

88

82-94

Plasma

90

84-96

 

 

 

 

Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Krueppel-like factor 5 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

93-103%

98-110%

92-102%

86-96%

EDTA plasma(n=5)

97-109%

102-113%

110-120%

104-116%

heparin plasma(n=5)

88-98%

 

119-129%

93-104%

98-108%

 

General Annotation


Sub Unit:
Interacts with WWP1.


Function:
Transcription factor that binds to GC box promoter elements. Activates the transcription of these genes.


Subcellular Location:
Nucleus


This product has not yet been referenced specifically in any publications.
Publishing research using this product ?
Please let us know and we will provide a credit for your next order.

[1].
"cDNA cloning and transcriptional properties of a novel GC box-binding protein, BTEB2."

[2].
"Regulatory polymorphism in transcription factor KLF5 at the MEF2 element alters the response to angiotensin II and is associated with human hypertension."

[3].
"Expression of Kruppel-like factor 5 gene in human brain and association of the gene with the susceptibility to schizophrenia."

[4].
"Analysis of KLF transcription factor family gene variants in type 2 diabetes."

[5].
"Ubiquitin-proteasome degradation of KLF5 transcription factor in cancer and untransformed epithelial cells."

[6].
"A gene encoding an intestinal-enriched member of the Krüppel-like factor family expressed in intestinal epithelial cells."

[7].
"Isolation and characterization of a gene encoding human Kruppel-like factor 5 (IKLF): binding to the CAAT/GT box of the mouse lactoferrin gene promoter."

[8].
"Shaking the family tree: identification of novel and biologically active alternatively spliced isoforms across the KLF family of transcription factors."

[9].
"A genome-wide association study identifies pancreatic cancer susceptibility loci on chromosomes 13q22.1, 1q32.1 and 5p15.33."

[10].
"The Fbw7/human CDC4 tumor suppressor targets proproliferative factor KLF5 for ubiquitination and degradation through multiple phosphodegron motifs."
Sample Data
Sample Data
Sample Data
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