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Home  >  ELISA Kit  >  Human C19orf80 ELISA Kit
C19orf80 (GeneName), Betatrophin (ProteinName), betat_human.
Product Name:

Human C19orf80/ Betatrophin ELISA Kit

 
Cat.#:

E11644h

Brand:
EIAab®
Regulatory Status:
Alternative:

Angiopoietin-like protein 8,Lipasin,Angptl8,UNQ599/PRO1185

Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
78-5000pg/mL
Sensitivity:
32pg/ml
Specificity:
Natural and recombinant human Betatrophin
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Research Area:
Immunology
Product Overview:
E11644h is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A Disintegrin and Metalloprotease 30 (Betatrophin) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Betatrophin. The ELISA analytical biochemical technique of the E11644h kit is based on Betatrophin antibody-Betatrophin antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Betatrophin antigen targets in samples. The ELISA Kit is designed to detect native, recombinant, Betatrophin. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Human C19orf80 ELISA Kit
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Product Datasheets
Datasheet:
Instruction:
MSDS:


Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤≤6.5%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤≤9.2%

Recovery
Recovery was determined by spiking various levels of Betatrophin into serum and plasma.

Sample Type

Average(%)

Recovery Range(%)

Serum

101

95-107

Plasma

103

97-109

 

 

 

 

Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Betatrophin and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

95-107%

86-99%

97-107%

87-96%

EDTA plasma(n=5)

96-106%

91-100%

102-112%

90-100%

heparin plasma(n=5)

92-102%

 

103-113%

86-96%

83-93%

 

General Annotation


Sub Unit:
Interacts with ANGPTL3.


Function:
Hormone that specifically promotes pancreatic beta cell proliferation and beta cell mass expansion, thereby improving glucose tolerance. Promotes pancreatic beta cell proliferation without insulin resistance. Also acts as a blood lipid regulator by regulating serum triglyceride levels; possibly by promoting ANGPTL3 cleavage.


Subcellular Location:
Secreted


Sample Data
Sample Data
Sample Data
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