C19orf80 (基因名), Betatrophin (蛋白名), betat_human.
产品名称:
Human C19orf80/ Betatrophin ELISA Kit
Betatrophin
货号:
E11644h
商标:
EIAab®
监管等级:
别名:
Angiopoietin-like protein 8, Lipasin, UNQ599/PRO1185, Angptl8
检测方法:
ELISA
实验类型:
Sandwich
检测范围:
78-5000pg/mL
灵敏度:
32pg/ml
特异性:
Natural and recombinant human Betatrophin
样品类型:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
样品数据:
登录.
实验步骤:
研究领域:
Immunology
精密度
批内差:已知浓度的3个样本在一个板子内重复检测20次,以评估批内精密度。
批内 CV: le;≤6.5%
批间差:已知浓度的3个样本在不同的板子上重复测定5次,以评估测定批间精密度。
批间 CV: le;≤9.2%
批内 CV: le;≤6.5%
批间差:已知浓度的3个样本在不同的板子上重复测定5次,以评估测定批间精密度。
批间 CV: le;≤9.2%
回收率
回收率:低、中和高浓度的分析物被掺入到血清或者血浆样本中,进行回收实验测定。
Sample Type | Average(%) | Recovery Range(%) |
Serum | 101 | 95-107 |
Plasma | 103 | 97-109 |
线性
线性:给定样本通过梯度稀释,每次稀释的测量值与理论值的比值。
Sample | 1:2 | 1:4 | 1:8 | 1:16 |
serum(n=5) | 95-107% | 86-99% | 97-107% | 87-96% |
EDTA plasma(n=5) | 96-106% | 91-100% | 102-112% | 90-100% |
heparin plasma(n=5) | 92-102%
| 103-113% | 86-96% | 83-93% |
通用注释
亚单元:
Interacts with ANGPTL3.
功能:
Hormone that specifically promotes pancreatic beta cell proliferation and beta cell mass expansion, thereby improving glucose tolerance. Promotes pancreatic beta cell proliferation without insulin resistance. Also acts as a blood lipid regulator by regulating serum triglyceride levels; possibly by promoting ANGPTL3 cleavage.
亚细胞位置:
Secreted
[1].
M Murillo, M Fonolleda, L Bosch, et al.
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[14].
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[16].
HX Li, MJ Xu, L Zhao, et al.
[18].
X Su, Gm Zhang, Y Cheng, et al.
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[20].
M Abu-Farha, J Abubaker, J Tuomilehto.
[21].
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[24].
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[34].
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[35].
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[37].
Abu-Farha M, Melhem M, Abubaker J, et al.
[38].
[39].
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[40].
Abu-Farha M, Abubaker J, Al-Khairi I, et al.
[42].
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[43].
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[44].
Chen C C, Susanto H, Chuang W H, et al.
[46].
Fang C, Huang Y, Guo H, et al.
[47].
"Positive Association Between Betatrophin and Diabetic Retinopathy Risk in Type 2 Diabetes Patients"
Wang Y Y, Zhang D, Jiang Z Y, et al.
[48].
S. Tokumoto, Y. Hamamoto, E. Okamura, et al
[50].
Hanson R L, Leti F, Tsinajinnie D, et al.
[51].
[53].
[54].
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[56].
[59].
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[61].
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[62].
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[64].
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[65].
[66].
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[68].
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[69].
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[72].
M Abu-Farha, P Cherian, D Sriraman, et al.
[73].
[1].
人Betatrophin(C19orf80)ELISA试剂盒可以做多少个样本?
人Betatrophin(C19orf80)ELISA试剂盒分为2种规格,96孔和48孔。96孔的试剂盒,标曲和样本都做复孔的话,可以检测40个样本。96孔的试剂盒,标曲和样本都不做复孔的话,可以检测88个样本。
[2].
人Betatrophin(C19orf80)ELISA试剂盒使用视频?
人Betatrophin(C19orf80)ELISA试剂盒实验操作视频在以下网址中,对每一步的实验步骤都做了演示,方便实验员能更好地理解ELISA实验的过程。
https://www.eiaab.com.cn/lesson-tech/805.html
https://www.eiaab.com.cn/lesson-tech/805.html
[3].
人Betatrophin(C19orf80)ELISA试剂盒是放在-20℃冰箱保存吗?
EIAab的人Betatrophin(C19orf80)ELISA试剂盒,洗涤液、底物、终止液保存于4℃,其余试剂-20℃冰箱保存。
[4].
人Betatrophin(C19orf80)ELISA试剂盒原理?
双抗体夹心法:用纯化的抗体包被微孔板,制成固相抗体,往包被有固相抗体的微孔中依次加入标准品或受检样本、生物素化抗体、HRP标记的亲和素,经过彻底洗涤后用底物TMB显色。用酶标仪在450nm波长下测定吸光度(OD值),计算样本浓度。
竞争法:用纯化的抗体包被微孔板,制成固相抗体,往包被有固相抗体的微孔中依次加入标准品或受检样本和生物素标记的目标分析物,受检标本中抗原与生物素标记抗原竞争结合有限的抗体。再加入HRP标记的亲和素,经过彻底洗涤后用底物TMB显色。用酶标仪在450nm波长下测定吸光度(OD值),计算样本浓度。
竞争法:用纯化的抗体包被微孔板,制成固相抗体,往包被有固相抗体的微孔中依次加入标准品或受检样本和生物素标记的目标分析物,受检标本中抗原与生物素标记抗原竞争结合有限的抗体。再加入HRP标记的亲和素,经过彻底洗涤后用底物TMB显色。用酶标仪在450nm波长下测定吸光度(OD值),计算样本浓度。
[5].
人Betatrophin(C19orf80)ELISA试剂盒中需要使用的样品量是多少?
夹心法100μL/孔,竞争法50μL/孔。如样本浓度过高时,应对样本进行稀释,以使稀释后的样本符合试剂盒的检测范围,计算时再乘以相应的稀释倍数。
[6].
如何分析人Betatrophin(C19orf80)ELISA试剂盒数据?
建议标准曲线,并计算样本浓度。对于elisa的曲线拟合,一般建议采用4参数曲线拟合,4参数曲线拟合通常更适合免疫分析。推荐使用专业软件进行曲线拟合,例如curve expert 1.3。根据样本的OD值由标曲查出相应的浓度,再乘以稀释倍数;或用标准物的浓度与OD值计算出标曲的回归方程式,将样本的OD值代入方程式,计算出样本浓度,再乘以稀释倍数,即为样本的实际浓度。以下链接是curve expert 1.3软件拟合曲线的方法。
https://www.eiaab.com.cn/news/502/
https://www.eiaab.com.cn/news/502/
[7].
人Betatrophin(C19orf80)ELISA试剂盒中是否包含人和动物的副产物,是否包含感染的或者传染性原料如HIV等?
除了抗体和稀释液中的BSA,不含其它人和动物的副产物,也不含感染材料。
[8].
收集人Betatrophin(C19orf80)ELISA试剂盒血浆样本,用什么作为抗凝剂?
一般建议用EDTA和肝素作为抗凝剂。
[9].
人Betatrophin(C19orf80)ELISA试剂盒酶标板可以拆成几部分?拆的时候是否需要避光,无菌?
人Betatrophin(C19orf80)ELISA试剂盒酶标板是8×12孔条,可拆卸,板子可以拆成12条,注意避免孔污染,不需要避光和无菌。暂时不用的板子,放回原来装的袋子里,密封保存。
[10].
人Betatrophin(C19orf80)ELISA试剂盒样本如何保存?
尽量检测新鲜样本。若无新鲜样本,则4℃保存1周,-20℃保存1个月,-80℃保存2个月。
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评论数 : 4
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admin
mafarha
customer
Arionas
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Betatrophin ELISA kit-Storage information
It is written in the operating instructions
that the reconstituted Standards and the detection reagents can be used
only once. Since I can remove the unused stripes that I don't need, will
I be able to store again these reagents for more distant use on the
remaining stripes?
admin
2019-06-05 16:09
2019-06-05 16:09
Yes, the reconstituted Standards and the detection reagents can be used only once, so we kindly recommend that you could reconstitute Standards, the detection reagents or wash buffer according you actual need. Once these reagents were reconstituted, they couldn’t be stored to use. They could only be stored in their raw status.
amy
2019-06-05 16:12
2019-06-05 16:12
0
0
Fantastic kit
1. M. Abu-Farha et al., Circulating ANGPTL8/Betatrophin Is Increased in Obesity and Reduced after Exercise Training. PLoS One 11, e0147367 (2016).
2. M. Abu-Farha et al., ANGPTL8/Betatrophin R59W variant is associated with higher glucose level in non-diabetic Arabs living in Kuwaits. Lipids Health Dis 15, 26 (2016).
3. M. Abu-Farha et al., Circulating angiopoietin-like protein 8 (betatrophin) association with HsCRP and metabolic syndrome. Cardiovasc Diabetol 15, 25 (2016).
4. M. Abu-Farha et al., Lack of associations between betatrophin/ANGPTL8 level and C-peptide in type 2 diabetic subjects. Cardiovasc Diabetol 14, 112 (2015).
5. M. Abu-Farha et al., Higher plasma betatrophin/ANGPTL8 level in Type 2 Diabetes subjects does not correlate with blood glucose or insulin resistance. Sci Rep 5, 10949 (2015).
mafarha
2016-03-08 04:06
2016-03-08 04:06
ye
2016-11-09 23:11 最佳回复
5
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333
2016-11-09 23:54
2
0
yeye
2016-11-09 23:37
2
1
TD-26 ELISA kit
Hi, Tech,
It is great to talk to you over the phone today. Could you please send me a copy of your kit instruction manual and your kit validation data (sensitivity, accuracy, sample linearity of dilution, reproducibility, normal serum/plasma sample concentrations, etc)?
Thanks!
customer
2015-01-07 18:23
2015-01-07 18:23
Thank you for your phone.
Please check the data in attachment for reference.There is the Validation Report for this elisa kit and you could check
https://www.scienceexchange.com/validations/29581
Please go to this product page for more information http://www.eiaab.com/entries/detail/TD26_HUMAN
Hope this is helpful for you.Please contact me as free if you need any further information.
star
2015-01-08 22:11 最佳回复
2015-01-08 22:11 最佳回复
5
0
i like zhis good!
2016-11-09 18:34
0
0
good
2016-11-09 18:31
0
0
good
2016-11-09 17:23
0
0
E11644h
I have a question about the ELISA kit E11644h. Can I also use it to measure mouse betatrophin/Angptl8? Or is there an ELISA kit specific for mouse betatrophin/Angptl8 provided by your company?
Thanks,
Arionas.
Arionas
2014-08-28 05:49
2014-08-28 05:49
Dear Arionas,Thank you very much for your inquiry and your interest in our products.We have the mouse Elisa kit for measuring mouse betatrophin/Angptl8.You could click this link for more information.http://www.eiaab.com/entries/detail/BETAT_MOUSE
I hope this helps, please let me know if you need any additional information or assistance.
star
2014-12-15 19:23 最佳回复
2014-12-15 19:23 最佳回复
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