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ASPRO (Shorten Name),
Product Name:

Human ASPRO/ Asprosin ELISA Kit

 
Cat.#:

E15190h

Brand:
EIAab®
Regulatory Status:
Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
1.56-100ng/mL
Sensitivity:
0.808ng/mL
Specificity:
Natural and recombinant human Asprosin
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Research Area:
Immunology
Product Overview:
E15190h is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A Disintegrin and Metalloprotease 30 (Asprosin) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Asprosin. The ELISA analytical biochemical technique of the E15190h kit is based on Asprosin antibody-Asprosin antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Asprosin antigen targets in samples. The ELISA Kit is designed to detect native, recombinant, Asprosin. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Human ASPRO ELISA Kit
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Product Datasheets
Datasheet:
Instruction:
MSDS:


Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤6.5%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤9.8%

Recovery
Recovery was determined by spiking various levels of Asprosin into serum and plasma.

Sample Type

Average(%)

Recovery Range(%)

Serum

111

105-117

Plasma

113

107-119

 

 

 

 

Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Asprosin and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

92-101%

103-113%

97-108%

90-99%

EDTA plasma(n=5)

108-117%

112-122%

93-103%

80-91%

heparin plasma(n=5)

96-106%

 

112-112%

99-108%

104-116%

 

General Annotation


Sub Unit:
Asprosin, a fasting-induced glucogenic protein homone that modulates hepatic glucose release. It is the C-terminal cleavage product of profibrillin, and encoded by the ultimate two exons of FBN1.


Function:
Circulating asprosin, a protein hormone, responds to low dietary glucose by triggering the release of liver glucose stores, and the reduction of asprosin protects against the hyperinsulinism associated with metabolic syndrome.


Location:
Asprosin circulates at nanomolar levels, a single dose of recombinant asprosin retains biological activity and elevates blood glucose and insulin levels. Liver is the target organ for asprosin to recruit. Asprosin increases hepatocyte glucose release through activating the G protein-cAMP-PKA pathway.


Sample Data
Sample Data
Sample Data
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