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ADAM8 (GeneName), Disintegrin and metalloproteinase domain-containing protein 8 (ProteinName), adam8_human.
Product Name:

Human ADAM8/ Disintegrin and metalloproteinase domain-containing protein 8 ELISA Kit

 
Cat.#:

E0620h

Brand:
EIAab®
Regulatory Status:
Alternative:

ADAM 8,Cell surface antigen MS2,CD156a,MS2

Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
78-5000pg/mL
Sensitivity:
39pg/mL
Specificity:
Natural and recombinant human Disintegrin and metalloproteinase domain-containing protein 8
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Research Area:
Cancer
Product Overview:
E0620h is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A Disintegrin and Metalloprotease 30 (Disintegrin and metalloproteinase domain-containing protein 8) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Disintegrin and metalloproteinase domain-containing protein 8. The ELISA analytical biochemical technique of the E0620h kit is based on Disintegrin and metalloproteinase domain-containing protein 8 antibody-Disintegrin and metalloproteinase domain-containing protein 8 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Disintegrin and metalloproteinase domain-containing protein 8 antigen targets in samples. The ELISA Kit is designed to detect native, recombinant, Disintegrin and metalloproteinase domain-containing protein 8. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Human ADAM8 ELISA Kit
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Product Datasheets
Instruction:
MSDS:


Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤3.9%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤9.7%

Recovery
Recovery was determined by spiking various levels of Disintegrin and metalloproteinase domain-containing protein 8 into serum and plasma.

Sample Type

Average(%)

Recovery Range(%)

Serum

95

89-101

Plasma

97

91-103

 

 

 

 

Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Disintegrin and metalloproteinase domain-containing protein 8 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

81-90%

88-98%

106-116%

111-121%

EDTA plasma(n=5)

102-110%

107-120%

103-112%

103-115%

heparin plasma(n=5)

106-115%

 

99-111%

101-110%

89-102%

 

General Annotation


Sub Unit:
Interacts with FST3.


Function:
Possible involvement in extravasation of leukocytes.


Subcellular Location:
Membrane Single-pass type I membrane protein


This product has not yet been referenced specifically in any publications.
Publishing research using this product ?
Please let us know and we will provide a credit for your next order.

[1].
"CD156 (human ADAM8): expression, primary amino acid sequence, and gene location."

[2].
"Evaluation of candidate stromal epithelial cross-talk genes identifies association between risk of serous ovarian cancer and TERT, a cancer susceptibility "hot-spot"."

[3].
"Involvement of a disintegrin and a metalloproteinase 8 (ADAM8) in osteoclastogenesis and pathological bone destruction."

[4].
"Autoactivation of human ADAM8: a novel pre-processing step is required for catalytic activity."

[5].
"Human parainfluenza virus type 2 (HPIV2) induced host ADAM8 expression in human salivary adenocarcinoma cell line (HSY) during cell fusion."

[6].
"ADAM8: a new therapeutic target for asthma."

[7].
"ADAM8 and its single nucleotide polymorphism 2662 T/G are associated with advanced atherosclerosis and fatal myocardial infarction: Tampere vascular study."

[8].
"ADAM8 in allergy."

[9].
"[Expression and clinical significance of a disintegrin and metalloprotease 8 (ADAM8) and epidermal growth factor receptor (EGFR) in non-small cell lung cancer]."

[10].
"Genes to diseases (G2D) computational method to identify asthma candidate genes."
Sample Data
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