TNFSF11 (基因名), Tumor necrosis factor ligand superfamily member 11 (蛋白名), TNF11_HUMAN.
产品名称:
Human TNFSF11/ Tumor necrosis factor ligand superfamily member 11 ELISA Kit
肿瘤坏死因子配体超家族成员11
货号:
E0855h
商标:
EIAab®
监管等级:
别名:
Osteoclast differentiation factor, Osteoprotegerin ligand, Receptor activator of nuclear factor kappa-B ligand, TNF-related activation-induced cytokine, ODF, OPGL, RANKL, TRANCE, CD254, OPGL, RANKL, TRANCE
检测方法:
ELISA
实验类型:
Sandwich
检测范围:
0.156-10ng/mL
灵敏度:
0.1ng/mL
特异性:
Natural and recombinant human Tumor necrosis factor ligand superfamily member 11
样品类型:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
样品数据:
登录.
实验步骤:
研究领域:
Immunology
精密度
批内差:已知浓度的3个样本在一个板子内重复检测20次,以评估批内精密度。
批内 CV: ≤6.1%
批间差:已知浓度的3个样本在不同的板子上重复测定5次,以评估测定批间精密度。
批间 CV: ≤7.9%
批内 CV: ≤6.1%
批间差:已知浓度的3个样本在不同的板子上重复测定5次,以评估测定批间精密度。
批间 CV: ≤7.9%
回收率
回收率:低、中和高浓度的分析物被掺入到血清或者血浆样本中,进行回收实验测定。
Sample Type |
Average(%) |
Recovery Range(%) |
Serum |
93 |
87-99 |
Plasma |
95 |
89-101 |
线性
线性:给定样本通过梯度稀释,每次稀释的测量值与理论值的比值。
Sample |
1:2 |
1:4 |
1:8 |
1:16 |
serum(n=5) |
92-102% |
86-96% |
107-117% |
93-102% |
EDTA plasma(n=5) |
106-116% |
83-96% |
102-113% |
102-110% |
heparin plasma(n=5) |
97-107%
|
89-101% |
108-118% |
114-124% |
通用注释
亚单元:
Homotrimer (By similarity). Interacts with TNFRSF11B (PubMed:22664871). Interacts with TNFRSF11A. Interacts with FBN1 (via N-terminal domain) in a Ca(+2)-dependent manner (By similarity).
功能:
Cytokine that binds to TNFRSF11B/OPG and to TNFRSF11A/RANK. Osteoclast differentiation and activation factor. Augments the ability of dendritic cells to stimulate naive T-cell proliferation. May be an important regulator of interactions between T-cells and dendritic cells and may play a role in the regulation of the T-cell-dependent immune response. May also play an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy (PubMed:22664871). Induces osteoclastogenesis by activating multiple signaling pathways in osteoclast precursor cells, chief among which is induction of long lasting oscillations in the intracellular concentration of Ca (2+) resulting in the activation of NFATC1, which translocates to the nucleus and induces osteoclast-specific gene transcription to allow differentiation of osteoclasts. During osteoclast differentiation, in a TMEM64 and ATP2A2-dependent manner induces activation of CREB1 and mitochondrial ROS generation necessary for proper osteoclast generation (By similarity).
亚细胞位置:
Tumor necrosis factor ligand superfamily member 11, soluble form
Secreted
[1].
Victoria Clérigues, Maria Isabel Guillén, Miguel Angel Castejón, Francisco Gomard, Vicente Mirabete, Maria José Alcaraz
From:
Biochemical Pharmacology
[2].
Sun Kyoung Lee, Kwang-Kyun Park, Jung Han Yoon Park, Soon Sung Lim, Won-Yoon Chung
From:
PhytotherapyReseach
[4].
[1].
人肿瘤坏死因子配体超家族成员11(TNFSF11)ELISA试剂盒可以做多少个样本?
人肿瘤坏死因子配体超家族成员11(TNFSF11)ELISA试剂盒分为2种规格,96孔和48孔。96孔的试剂盒,标曲和样本都做复孔的话,可以检测40个样本。96孔的试剂盒,标曲和样本都不做复孔的话,可以检测88个样本。
[2].
人肿瘤坏死因子配体超家族成员11(TNFSF11)ELISA试剂盒使用视频?
人肿瘤坏死因子配体超家族成员11(TNFSF11)ELISA试剂盒实验操作视频在以下网址中,对每一步的实验步骤都做了演示,方便实验员能更好地理解ELISA实验的过程。
https://www.eiaab.com.cn/lesson-tech/805.html
https://www.eiaab.com.cn/lesson-tech/805.html
[3].
人肿瘤坏死因子配体超家族成员11(TNFSF11)ELISA试剂盒是放在-20℃冰箱保存吗?
EIAab的人肿瘤坏死因子配体超家族成员11(TNFSF11)ELISA试剂盒,洗涤液、底物、终止液保存于4℃,其余试剂-20℃冰箱保存。
[4].
人肿瘤坏死因子配体超家族成员11(TNFSF11)ELISA试剂盒原理?
双抗体夹心法:用纯化的抗体包被微孔板,制成固相抗体,往包被有固相抗体的微孔中依次加入标准品或受检样本、生物素化抗体、HRP标记的亲和素,经过彻底洗涤后用底物TMB显色。用酶标仪在450nm波长下测定吸光度(OD值),计算样本浓度。
竞争法:用纯化的抗体包被微孔板,制成固相抗体,往包被有固相抗体的微孔中依次加入标准品或受检样本和生物素标记的目标分析物,受检标本中抗原与生物素标记抗原竞争结合有限的抗体。再加入HRP标记的亲和素,经过彻底洗涤后用底物TMB显色。用酶标仪在450nm波长下测定吸光度(OD值),计算样本浓度。
竞争法:用纯化的抗体包被微孔板,制成固相抗体,往包被有固相抗体的微孔中依次加入标准品或受检样本和生物素标记的目标分析物,受检标本中抗原与生物素标记抗原竞争结合有限的抗体。再加入HRP标记的亲和素,经过彻底洗涤后用底物TMB显色。用酶标仪在450nm波长下测定吸光度(OD值),计算样本浓度。
[5].
人肿瘤坏死因子配体超家族成员11(TNFSF11)ELISA试剂盒中需要使用的样品量是多少?
夹心法100μL/孔,竞争法50μL/孔。如样本浓度过高时,应对样本进行稀释,以使稀释后的样本符合试剂盒的检测范围,计算时再乘以相应的稀释倍数。
[6].
如何分析人肿瘤坏死因子配体超家族成员11(TNFSF11)ELISA试剂盒数据?
建议标准曲线,并计算样本浓度。对于elisa的曲线拟合,一般建议采用4参数曲线拟合,4参数曲线拟合通常更适合免疫分析。推荐使用专业软件进行曲线拟合,例如curve expert 1.3。根据样本的OD值由标曲查出相应的浓度,再乘以稀释倍数;或用标准物的浓度与OD值计算出标曲的回归方程式,将样本的OD值代入方程式,计算出样本浓度,再乘以稀释倍数,即为样本的实际浓度。以下链接是curve expert 1.3软件拟合曲线的方法。
https://www.eiaab.com.cn/news/502/
https://www.eiaab.com.cn/news/502/
[7].
人肿瘤坏死因子配体超家族成员11(TNFSF11)ELISA试剂盒中是否包含人和动物的副产物,是否包含感染的或者传染性原料如HIV等?
除了抗体和稀释液中的BSA,不含其它人和动物的副产物,也不含感染材料。
[8].
收集人肿瘤坏死因子配体超家族成员11(TNFSF11)ELISA试剂盒血浆样本,用什么作为抗凝剂?
一般建议用EDTA和肝素作为抗凝剂。
[9].
人肿瘤坏死因子配体超家族成员11(TNFSF11)ELISA试剂盒酶标板可以拆成几部分?拆的时候是否需要避光,无菌?
人肿瘤坏死因子配体超家族成员11(TNFSF11)ELISA试剂盒酶标板是8×12孔条,可拆卸,板子可以拆成12条,注意避免孔污染,不需要避光和无菌。暂时不用的板子,放回原来装的袋子里,密封保存。
[10].
人肿瘤坏死因子配体超家族成员11(TNFSF11)ELISA试剂盒样本如何保存?
尽量检测新鲜样本。若无新鲜样本,则4℃保存1周,-20℃保存1个月,-80℃保存2个月。
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the difference between RANKL and sRANKL
Hi,
I am interested in your ELISA kits for measuring RANKL in various samples.
Can you please tell me what the difference is between these two kits:
E0855h
Human receptor activator of nuclear factor kappa B ligand ELISA Kit
E0470h
Human soluble receptor activator of nuclear factor-kB ligand ELISA Kit
Is there a specific reason to choose one of these kits rather than the other? Once I have this information I will place an order with your distributor in Australia.
Thanks,
customer
2014-01-22 22:42
2014-01-22 22:42
Thank you for your enquiry!E0855h Human receptor activator of nuclear factor kappa B ligand ELISA Kit ,
Specificity: detects endogenous levels of total RANKL protein.
E0470h Human soluble receptor activator of nuclear factor-kB ligand ELISA Kit ,
Specificity: detects hsRANK-L (human soluble receptor activator of NF-Kappa B Ligand).
Human sRANKL is a 20.0 kDa polypeptide comprising the TNF homologous region of RANKL (176 amino acid residues).
What is your research purpose?I will find the suitable kit for your research.
Please contact me as free if you need any further information.
2014-01-23 01:08
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