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MYLK (基因名), Myosin light chain kinase, smooth muscle (蛋白名), MYLK_BOVIN.

产品名称:

Bovine MYLK/ Myosin light chain kinase, smooth muscle ELISA Kit
平滑肌肌球蛋白轻链激

货号:

E1106b

商标:

EIAab^®

监管等级:

RUO

别名:

Telokin, MLCK

检测方法:

ELISA

实验类型:

Sandwich

检测范围:

78-5000pg/mL

灵敏度:

33pg/mL

特异性:

Natural and recombinant bovine Myosin light chain kinase, smooth muscle

样品类型:

Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids

样品数据:

实验步骤:

研究领域:

Signal Transduction

规格 & 价格:

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产品说明书

数据表:

说明书:

MSDS:

在线询价

数据
文献 ( 3 )
序列 / 3D
常见问题解答
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通用注释

亚单元:

All isoforms including Telokin bind calmodulin. Interacts with SVIL (By similarity). Interacts with CTTN; this interaction is reduced during thrombin-induced endothelial cell (EC) contraction but is promoted by the barrier-protective agonist sphingosine 1-phosphate (S1P) within lamellipodia. A complex made of ABL1, CTTN and MYLK regulates cortical actin-based cytoskeletal rearrangement critical to sphingosine 1-phosphate (S1P)-mediated endothelial cell (EC) barrier enhancement. Binds to NAA10/ARD1 and PTK2B/PYK2.

功能:

Calcium/calmodulin-dependent myosin light chain kinase implicated in smooth muscle contraction via phosphorylation of myosin light chains (MLC). Also regulates actin-myosin interaction through a non-kinase activity. Phosphorylates PTK2B/PYK2 and myosin light-chains. Involved in the inflammatory response (e.g. apoptosis, vascular permeability, leukocyte diapedesis), cell motility and morphology, airway hyperreactivity and other activities relevant to asthma. Required for tonic airway smooth muscle contraction that is necessary for physiological and asthmatic airway resistance. Necessary for gastrointestinal motility. Implicated in the regulation of endothelial as well as vascular permeability, probably via the regulation of cytoskeletal rearrangements. In the nervous system it has been shown to control the growth initiation of astrocytic processes in culture and to participate in transmitter release at synapses formed between cultured sympathetic ganglion cells. Critical participant in signaling sequences that result in fibroblast apoptosis. Plays a role in the regulation of epithelial cell survival. Required for epithelial wound healing, especially during actomyosin ring contraction during purse-string wound closure. Mediates RhoA-dependent membrane blebbing. Triggers TRPC5 channel activity in a calcium-dependent signaling, by inducing its subcellular localization at the plasma membrane. Promotes cell migration (including tumor cells) and tumor metastasis. PTK2B/PYK2 activation by phosphorylation mediates ITGB2 activation and is thus essential to trigger neutrophil transmigration during acute lung injury (ALI). May regulate optic nerve head astrocyte migration. Probably involved in mitotic cytoskeletal regulation. Regulates tight junction probably by modulating ZO-1 exchange in the perijunctional actomyosin ring. Mediates burn-induced microvascular barrier injury; triggers endothelial contraction in the development of microvascular hyperpermeability by phosphorylating MLC. Essential for intestinal barrier dysfunction. Mediates Giardia spp.-mediated reduced epithelial barrier function during giardiasis intestinal infection via reorganization of cytoskeletal F-actin and tight junctional ZO-1. Necessary for hypotonicity-induced Ca(2+) entry and subsequent activation of volume-sensitive organic osmolyte/anion channels (VSOAC) in cervical cancer cells.

亚细胞位置:

Cytoplasm Cell projection Lamellipodium Cleavage furrow Cytoplasm Cytoskeleton Localized to stress fibers during interphase and to the cleavage furrow during mitosis.

数据库链接

UniGene:

Bt.39894

KEGG:

bta:338037

Pfam:

PF00041

Uniprot:

Q28824

相关产品

序列长度:

1176

序列:

仅供参考，详细请咨询（services@eiaab.com）

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ATCTAELIVE | TMEEGEGEGG | EEEEEE

3D 模型:

3D 结构:

免疫原序列:

验证序列:

[1].

"Chloroorganic (DDT) and organophosphate (malathion) insecticides impair the motor function of the bovine cervix"

M.H. Wrobel, J. Mlynarczuk.

From: Toxicology and Applied Pharmacology

[2].

"Do commonly used herbicides (atrazine and glyphosate) have the potential to impair the contractions, prostaglandin releasing and conducting of oxytocin signal at the bovine cervix in vitro?"

M H Wróbel, J Młynarczuk, R Rękawiecki, et al.

From: Theriogenology

[3].

"Do chlorinated insecticides (aldrin and DDT) or products of their transformations (dieldrin and DDE) impair signal transfer from regulators (oxytocin and relaxin) of bovine myometrium motility in vitro?"

W H Wrobel.

From: Environmental Research

[1].

牛平滑肌肌球蛋白轻链激(MYLK)ELISA试剂盒可以做多少个样本？

牛平滑肌肌球蛋白轻链激(MYLK)ELISA试剂盒分为2种规格，96孔和48孔。96孔的试剂盒，标曲和样本都做复孔的话，可以检测40个样本。96孔的试剂盒，标曲和样本都不做复孔的话，可以检测88个样本。

[2].

牛平滑肌肌球蛋白轻链激(MYLK)ELISA试剂盒使用视频？

牛平滑肌肌球蛋白轻链激(MYLK)ELISA试剂盒实验操作视频在以下网址中，对每一步的实验步骤都做了演示，方便实验员能更好地理解ELISA实验的过程。
https://www.eiaab.com.cn/lesson-tech/805.html

[3].

牛平滑肌肌球蛋白轻链激(MYLK)ELISA试剂盒是放在-20℃冰箱保存吗？

EIAab的牛平滑肌肌球蛋白轻链激(MYLK)ELISA试剂盒，洗涤液、底物、终止液保存于4℃，其余试剂-20℃冰箱保存。

[4].

牛平滑肌肌球蛋白轻链激(MYLK)ELISA试剂盒原理？

双抗体夹心法：用纯化的抗体包被微孔板，制成固相抗体，往包被有固相抗体的微孔中依次加入标准品或受检样本、生物素化抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。用酶标仪在450nm波长下测定吸光度(OD值）,计算样本浓度。
竞争法：用纯化的抗体包被微孔板，制成固相抗体，往包被有固相抗体的微孔中依次加入标准品或受检样本和生物素标记的目标分析物，受检标本中抗原与生物素标记抗原竞争结合有限的抗体。再加入HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。用酶标仪在450nm波长下测定吸光度(OD值）,计算样本浓度。

[5].

牛平滑肌肌球蛋白轻链激(MYLK)ELISA试剂盒中需要使用的样品量是多少？

夹心法100μL/孔，竞争法50μL/孔。如样本浓度过高时，应对样本进行稀释，以使稀释后的样本符合试剂盒的检测范围，计算时再乘以相应的稀释倍数。

[6].

如何分析牛平滑肌肌球蛋白轻链激(MYLK)ELISA试剂盒数据？

建议标准曲线，并计算样本浓度。对于elisa的曲线拟合，一般建议采用4参数曲线拟合，4参数曲线拟合通常更适合免疫分析。推荐使用专业软件进行曲线拟合，例如curve expert 1.3。根据样本的OD值由标曲查出相应的浓度，再乘以稀释倍数；或用标准物的浓度与OD值计算出标曲的回归方程式，将样本的OD值代入方程式，计算出样本浓度，再乘以稀释倍数，即为样本的实际浓度。以下链接是curve expert 1.3软件拟合曲线的方法。
https://www.eiaab.com.cn/news/502/

[7].

牛平滑肌肌球蛋白轻链激(MYLK)ELISA试剂盒中是否包含人和动物的副产物，是否包含感染的或者传染性原料如HIV等？

除了抗体和稀释液中的BSA，不含其它人和动物的副产物，也不含感染材料。

[8].

收集牛平滑肌肌球蛋白轻链激(MYLK)ELISA试剂盒血浆样本，用什么作为抗凝剂？

一般建议用EDTA和肝素作为抗凝剂。

[9].

牛平滑肌肌球蛋白轻链激(MYLK)ELISA试剂盒酶标板可以拆成几部分？拆的时候是否需要避光，无菌？

牛平滑肌肌球蛋白轻链激(MYLK)ELISA试剂盒酶标板是8×12孔条，可拆卸，板子可以拆成12条，注意避免孔污染，不需要避光和无菌。暂时不用的板子，放回原来装的袋子里，密封保存。

[10].

牛平滑肌肌球蛋白轻链激(MYLK)ELISA试剂盒样本如何保存？

尽量检测新鲜样本。若无新鲜样本，则4℃保存1周，-20℃保存1个月，-80℃保存2个月。

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Bovine MYLK/ Myosin light chain kinase, smooth muscle ELISA Kit 平滑肌肌球蛋白轻链激

E1106b

Telokin, MLCK

Bovine MYLK/ Myosin light chain kinase, smooth muscle ELISA Kit
平滑肌肌球蛋白轻链激