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MAP1LC3A (基因名), Microtubule-associated proteins 1A/1B light chain 3A (蛋白名), MLP3A_BOVIN.

产品名称:

Bovine MAP1LC3A/ Microtubule-associated proteins 1A/1B light chain 3A ELISA Kit
HumanMicrotubule-associatedproteins1A/1Blightchain3A

货号:

E2879b

商标:

EIAab^®

监管等级:

RUO

别名:

Autophagy-related protein LC3 A, Autophagy-related ubiquitin-like modifier LC3 A, MAP1 light chain 3-like protein 1, MAP1A/MAP1B light chain 3 A, Microtubule-associated protein 1 light chain 3 alpha, MAP1A/MAP1B LC3 A

检测方法:

ELISA

特异性:

Natural and recombinant bovine Microtubule-associated proteins 1A/1B light chain 3A

样品类型:

Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids

样品数据:

研究领域:

Cancer

规格 & 价格:

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产品说明书

在线询价

数据
文献
序列 / 3D
常见问题解答
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通用注释

亚单元:

3 different light chains, LC1, LC2 and LC3, can associate with MAP1A and MAP1B proteins (By similarity). Interacts with TP53INP1 and TP53INP2. Directly interacts with SQSTM1; this interaction leads to MAP1LC3A recruitment to inclusion bodies containing polyubiquitinated protein aggregates and to inclusion body degradation by autophagy. Interacts with ATG13. Interacts with ULK1. Interacts with TBC1D5. Found in a complex with UBQLN1 and UBQLN2. Interacts with UBQLN4 (via STI1 1 and 2 domains). Interacts with UBQLN1 in the presence of UBQLN4. Interacts with TRIM5. Interacts with MEFV. Interacts with FAM134A, FAM134B and FAM134C.

功能:

Ubiquitin-like modifier involved in formation of autophagosomal vacuoles (autophagosomes). Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation.

亚细胞位置:

Cytoplasm Cytoskeleton Endomembrane system Lipid-anchor Cytoplasmic vesicle Autophagosome membrane Lipid-anchor Cytoplasmic vesicle Autophagosome LC3-II binds to the autophagic membranes.

数据库链接

UniGene:

Bt.8073

SMR:

Q2HJ23

STRING:

9913.ENSBTAP00000008072

KEGG:

bta:514547

Pfam:

PF02991

Uniprot:

Q2HJ23

相关产品

Human MAP1LC3A ELISA Kit

Mouse Map1lc3a ELISA Kit

Rat Map1lc3a ELISA Kit

Human MAP1LC3A CLIA Kit

Mouse Map1lc3a CLIA Kit

序列长度:

121

序列:

仅供参考，详细请咨询（services@eiaab.com）

50:

100:

121:

QEKDEDGFLY | MVYASQETFG | F

3D 模型:

3D 结构:

免疫原序列:

验证序列:

该产品尚未在任何出版物中被引用。

[1].

牛HumanMicrotubule-associatedproteins1A/1Blightchain3A(MAP1LC3A)ELISA试剂盒可以做多少个样本？

牛HumanMicrotubule-associatedproteins1A/1Blightchain3A(MAP1LC3A)ELISA试剂盒分为2种规格，96孔和48孔。96孔的试剂盒，标曲和样本都做复孔的话，可以检测40个样本。96孔的试剂盒，标曲和样本都不做复孔的话，可以检测88个样本。

[2].

牛HumanMicrotubule-associatedproteins1A/1Blightchain3A(MAP1LC3A)ELISA试剂盒使用视频？

牛HumanMicrotubule-associatedproteins1A/1Blightchain3A(MAP1LC3A)ELISA试剂盒实验操作视频在以下网址中，对每一步的实验步骤都做了演示，方便实验员能更好地理解ELISA实验的过程。
https://www.eiaab.com.cn/lesson-tech/805.html

[3].

牛HumanMicrotubule-associatedproteins1A/1Blightchain3A(MAP1LC3A)ELISA试剂盒是放在-20℃冰箱保存吗？

EIAab的牛HumanMicrotubule-associatedproteins1A/1Blightchain3A(MAP1LC3A)ELISA试剂盒，洗涤液、底物、终止液保存于4℃，其余试剂-20℃冰箱保存。

[4].

牛HumanMicrotubule-associatedproteins1A/1Blightchain3A(MAP1LC3A)ELISA试剂盒原理？

双抗体夹心法：用纯化的抗体包被微孔板，制成固相抗体，往包被有固相抗体的微孔中依次加入标准品或受检样本、生物素化抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。用酶标仪在450nm波长下测定吸光度(OD值）,计算样本浓度。
竞争法：用纯化的抗体包被微孔板，制成固相抗体，往包被有固相抗体的微孔中依次加入标准品或受检样本和生物素标记的目标分析物，受检标本中抗原与生物素标记抗原竞争结合有限的抗体。再加入HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。用酶标仪在450nm波长下测定吸光度(OD值）,计算样本浓度。

[5].

牛HumanMicrotubule-associatedproteins1A/1Blightchain3A(MAP1LC3A)ELISA试剂盒中需要使用的样品量是多少？

夹心法100μL/孔，竞争法50μL/孔。如样本浓度过高时，应对样本进行稀释，以使稀释后的样本符合试剂盒的检测范围，计算时再乘以相应的稀释倍数。

[6].

如何分析牛HumanMicrotubule-associatedproteins1A/1Blightchain3A(MAP1LC3A)ELISA试剂盒数据？

建议标准曲线，并计算样本浓度。对于elisa的曲线拟合，一般建议采用4参数曲线拟合，4参数曲线拟合通常更适合免疫分析。推荐使用专业软件进行曲线拟合，例如curve expert 1.3。根据样本的OD值由标曲查出相应的浓度，再乘以稀释倍数；或用标准物的浓度与OD值计算出标曲的回归方程式，将样本的OD值代入方程式，计算出样本浓度，再乘以稀释倍数，即为样本的实际浓度。以下链接是curve expert 1.3软件拟合曲线的方法。
https://www.eiaab.com.cn/news/502/

[7].

牛HumanMicrotubule-associatedproteins1A/1Blightchain3A(MAP1LC3A)ELISA试剂盒中是否包含人和动物的副产物，是否包含感染的或者传染性原料如HIV等？

除了抗体和稀释液中的BSA，不含其它人和动物的副产物，也不含感染材料。

[8].

收集牛HumanMicrotubule-associatedproteins1A/1Blightchain3A(MAP1LC3A)ELISA试剂盒血浆样本，用什么作为抗凝剂？

一般建议用EDTA和肝素作为抗凝剂。

[9].

牛HumanMicrotubule-associatedproteins1A/1Blightchain3A(MAP1LC3A)ELISA试剂盒酶标板可以拆成几部分？拆的时候是否需要避光，无菌？

牛HumanMicrotubule-associatedproteins1A/1Blightchain3A(MAP1LC3A)ELISA试剂盒酶标板是8×12孔条，可拆卸，板子可以拆成12条，注意避免孔污染，不需要避光和无菌。暂时不用的板子，放回原来装的袋子里，密封保存。

[10].

牛HumanMicrotubule-associatedproteins1A/1Blightchain3A(MAP1LC3A)ELISA试剂盒样本如何保存？

尽量检测新鲜样本。若无新鲜样本，则4℃保存1周，-20℃保存1个月，-80℃保存2个月。

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Bovine MAP1LC3A/ Microtubule-associated proteins 1A/1B light chain 3A ELISA Kit HumanMicrotubule-associatedproteins1A/1Blightchain3A

E2879b

Autophagy-related protein LC3 A, Autophagy-related ubiquitin-like modifier LC3 A, MAP1 light chain 3-like protein 1, MAP1A/MAP1B light chain 3 A, Microtubule-associated protein 1 light chain 3 alpha, MAP1A/MAP1B LC3 A

Bovine MAP1LC3A/ Microtubule-associated proteins 1A/1B light chain 3A ELISA Kit
HumanMicrotubule-associatedproteins1A/1Blightchain3A