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首页  >  酶联免疫试剂盒  >  Mouse Mapk15 ELISA Kit
Mapk15 (基因名), Mitogen-activated protein kinase 15 (蛋白名), MK15_MOUSE.
产品名称:

Mouse Mapk15/ Mitogen-activated protein kinase 15 ELISA Kit
促分裂原活化蛋白激酶15

货号:

E10361m

商标:
EIAab®
监管等级:
别名:

MAP kinase 15, Extracellular signal-regulated kinase 7, ERK-7, Erk7, Extracellular signal-regulated kinase 8, ERK-8, Erk8

检测方法:
ELISA
实验类型:
Sandwich
检测范围:
78-5000pg/mL
灵敏度:
39.7pg/mL
特异性:
Natural and recombinant mouse Mitogen-activated protein kinase 15
样品类型:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
样品数据:
实验步骤:
实验步骤
研究领域:
-
Mouse Mapk15 ELISA Kit
规格 & 价格: cart
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Mouse Mapk15 ELISA Kit
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产品说明书
说明书: 下载说明书
MSDS: MSDS


精密度
批内差:已知浓度的3个样本在一个板子内重复检测20次,以评估批内精密度。
批内 CV: ≤7.8%
批间差:已知浓度的3个样本在不同的板子上重复测定5次,以评估测定批间精密度。
批间 CV: ≤10.0%
回收率
回收率:低、中和高浓度的分析物被掺入到血清或者血浆样本中,进行回收实验测定。

Sample Type

Average(%)

Recovery Range(%)

Serum

91

85-97

Plasma

93

87-99

 

 

 

 

线性
线性:给定样本通过梯度稀释,每次稀释的测量值与理论值的比值。

Sample

1:2

1:4

1:8

1:16

serum(n=5)

96-105%

81-81%

103-112%

102-112%

EDTA plasma(n=5)

88-88%

102-111%

102-112%

108-117%

heparin plasma(n=5)

111-121%

 

89-99%

99-109%

80-92%

 

通用注释


亚单元:
Interacts with TGFB1I1 (PubMed:16624805). Interacts with CSK/c-Src, ABL1 and RET. Interacts with GABARAP, MAP1LC3B and GABARAPL1; controls, in a kinase-dependent fashion, both basal and starvation-induced autophagy. Interacts with ESRRA; promotes re-localization of ESRRA to the cytoplasm through a XPO1-dependent mechanism then inhibits ESRRA transcriptional activity. Interacts with PCNA; the interaction is chromatin binding- and kinase activity-dependent and prevents MDM2-mediated PCNA destruction by inhibiting the association of PCNA with MDM2 (By similarity). Interacts with DVL2 (PubMed:25823377). Interacts with CLIC3; MAPK15 does not phosphorylates CLIC3 (By similarity).


功能:
Atypical MAPK protein that regulates several process such as autophagy, ciliogenesis, protein trafficking/secretion and genome integrity, in a kinase activity-dependent manner (By similarity) (PubMed:25823377). Controls both, basal and starvation-induced autophagy throught its interaction with GABARAP, MAP1LC3B and GABARAPL1 leading to autophagosome formation, SQSTM1 degradation and reduced MAP1LC3B inhibitory phosphorylation. Regulates primary cilium formation and the localization of ciliary proteins involved in cilium structure, transport, and signaling. Prevents the relocation of the sugar-adding enzymes from the Golgi to the endoplasmic reticulum, thereby restricting the production of sugar-coated proteins. Upon amino-acid starvation, mediates transitional endoplasmic reticulum site disassembly and inhibition of secretion. Binds to chromatin leading to MAPK15 activation and interaction with PCNA, that which protects genomic integrity by inhibiting MDM2-mediated degradation of PCNA. Regulates DA transporter (DAT) activity and protein expression via activation of RhoA. In response to H(2)O(2) treatment phosphorylates ELAVL1, thus preventing it from binding to the PDCD4 3'UTR and rendering the PDCD4 mRNA accessible to miR-21 and leading to its degradation and loss of protein expression (By similarity). Also functions in a kinase activity-independent manner as a negative regulator of growth (By similarity). Phosphorylates in vitro FOS and MBP (By similarity). During oocyte maturation, plays a key role in the microtubule organization and meiotic cell cycle progression in oocytes, fertilized eggs, and early embryos (PubMed:23351492). Interacts with ESRRA promoting its re-localization from the nucleus to the cytoplasm and then prevents its transcriptional activity (By similarity).


亚细胞位置:
Cytoplasm Cytoskeleton Cilium basal body Cell junction Tight junction Cytoplasm Cytoskeleton Microtubule organizing center Centrosome Centriole Cytoplasmic vesicle Autophagosome Golgi apparatus Nucleus Cytoplasm Cytoplasm Cytoskeleton Spindle Co-localizes to the cytoplasm only in presence of ESRRA. Translocates to the nucleus upon activation (By similarity). At prometaphase I, metaphase I (MI), anaphase I, telophase I, and metaphase II (MII) stages, is stably detected at the spindle (PubMed:23351492).


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