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Mapk1 (基因名), Mitogen-activated protein kinase 1 (蛋白名), MK01_RAT.
产品名称:

Rat Mapk1/ Mitogen-activated protein kinase 1 ELISA Kit
促分裂原激活蛋白激酶1

货号:

E1206r

商标:
EIAab®
监管等级:
别名:

ERT1, Extracellular signal-regulated kinase 2, MAP kinase isoform p42, Mitogen-activated protein kinase 2, ERK-2, p42-MAPK, MAP kinase 2, MAP kinase 1, Erk2, Mapk, Prkm1

检测方法:
ELISA
实验类型:
Sandwich
检测范围:
31.2-2000pg/mL
灵敏度:
15.74pg/mL
特异性:
Natural and recombinant rat Mitogen-activated protein kinase 1
样品类型:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
样品数据:
实验步骤:
实验步骤
研究领域:
Neurosciences
Rat Mapk1 ELISA Kit
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Rat Mapk1 ELISA Kit
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产品说明书
数据表: 下载说明书
说明书: 下载说明书
MSDS: MSDS
在线询价


精密度
批内差:已知浓度的3个样本在一个板子内重复检测20次,以评估批内精密度。
批内 CV: ≤4.5%
批间差:已知浓度的3个样本在不同的板子上重复测定5次,以评估测定批间精密度。
批间 CV: ≤7.9%
回收率
回收率:低、中和高浓度的分析物被掺入到血清或者血浆样本中,进行回收实验测定。

Sample Type

Average(%)

Recovery Range(%)

Serum

104

98-110

Plasma

106

100-112

 

 

 

 

线性
线性:给定样本通过梯度稀释,每次稀释的测量值与理论值的比值。

Sample

1:2

1:4

1:8

1:16

serum(n=5)

84-94%

89-101%

91-102%

92-101%

EDTA plasma(n=5)

95-105%

106-119%

100-110%

92-101%

heparin plasma(n=5)

103-111%

 

106-116%

84-93%

102-112%

 

通用注释


亚单元:
Binds both upstream activators and downstream substrates in multimolecular complexes. Interacts with ADAM15, ARHGEF2, DAPK1 (via death domain), HSF4, IER3, IPO7, MKNK2, MORG1, NISCH, PEA15, SGK1, and isoform 1 of NEK2 (By similarity). Interacts with DUSP6 (PubMed:16567630). Interacts with ARRB2 (PubMed:11226259). Interacts (phosphorylated form) with CAV2 ('Tyr-19'-phosphorylated form); the interaction, promoted by insulin, leads to nuclear location and MAPK1 activation (PubMed:19778377, PubMed:19427337). MKNK2 isoform 1 binding prevents from dephosphorylation and inactivation (By similarity). Interacts with DCC (PubMed:21070949). The phosphorylated form interacts with PML (By similarity). Interacts with STYX (By similarity). Interacts with CDK2AP2 (PubMed:12944431).


功能:
Acts as a transcriptional repressor. Binds to a [GC]AAA[GC] consensus sequence. Repress the expression of interferon gamma-induced genes. Seems to bind to the promoter of CCL5, DMP1, IFIH1, IFITM1, IRF7, IRF9, LAMP3, OAS1, OAS2, OAS3 and STAT1. Transcriptional activity is independent of kinase activity.


亚细胞位置:
Cytoplasm Cytoskeleton Spindle Nucleus Cytoplasm Cytoskeleton Microtubule organizing center Centrosome Cytoplasm Associated with the spindle during prometaphase and metaphase. PEA15-binding and phosphorylated DAPK1 promote its cytoplasmic retention. Phosphorylation at Ser- 244 and Ser-246 as well as autophosphorylation at Thr-188 promote nuclear localization.


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