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首页  >  酶联免疫试剂盒  >  Human MAPK1 ELISA Kit
MAPK1 (基因名), Mitogen-activated protein kinase 1 (蛋白名), MK01_HUMAN.
产品名称:

Human MAPK1/ Mitogen-activated protein kinase 1 ELISA Kit
促分裂原激活蛋白激酶1

货号:

E1206h

商标:
EIAab®
监管等级:
别名:

MAP kinase 1, ERT1, Extracellular signal-regulated kinase 2, ERK-2, MAP kinase isoform p42, p42-MAPK, Mitogen-activated protein kinase 2, MAP kinase 2, ERK2, PRKM1, PRKM2

检测方法:
ELISA
实验类型:
Sandwich
检测范围:
0.156-10ng/mL
灵敏度:
0.089ng/mL
特异性:
Natural and recombinant human Mitogen-activated protein kinase 1
样品类型:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
样品数据:
实验步骤:
实验步骤
研究领域:
Neurosciences
Human MAPK1 ELISA Kit
规格 & 价格: cart
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Human MAPK1 ELISA Kit
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产品说明书
说明书: 下载说明书
MSDS: MSDS


精密度
批内差:已知浓度的3个样本在一个板子内重复检测20次,以评估批内精密度。
批内 CV: ≤4.7%
批间差:已知浓度的3个样本在不同的板子上重复测定5次,以评估测定批间精密度。
批间 CV: ≤8.2%
回收率
回收率:低、中和高浓度的分析物被掺入到血清或者血浆样本中,进行回收实验测定。

Sample Type

Average(%)

Recovery Range(%)

Serum

99

93-105

Plasma

101

95-107

 

 

 

 

线性
线性:给定样本通过梯度稀释,每次稀释的测量值与理论值的比值。

Sample

1:2

1:4

1:8

1:16

serum(n=5)

100-113%

98-108%

84-94%

97-109%

EDTA plasma(n=5)

99-110%

91-102%

98-106%

110-120%

heparin plasma(n=5)

100-110%

 

104-114%

96-106%

81-94%

 

通用注释


亚单元:
Binds both upstream activators and downstream substrates in multimolecular complexes. Binds to HIV-1 Nef through its SH3 domain. This interaction inhibits its tyrosine-kinase activity. Interacts with ADAM15, ARHGEF2, ARRB2, DAPK1 (via death domain), HSF4, IER3, IPO7, DUSP6, NISCH, SGK1, and isoform 1 of NEK2. Interacts (phosphorylated form) with CAV2 ('Tyr-19'-phosphorylated form); the interaction, promoted by insulin, leads to nuclear location and MAPK1 activation. Interacts with MORG1, PEA15 and MKNK2 (By similarity). MKNK2 isoform 1 binding prevents from dephosphorylation and inactivation (By similarity). Interacts with DCC (By similarity). The phosphorylated form interacts with PML (isoform PML-4). Interacts with STYX. Interacts with CDK2AP2.


功能:
Acts as a transcriptional repressor. Binds to a [GC]AAA[GC] consensus sequence. Repress the expression of interferon gamma-induced genes. Seems to bind to the promoter of CCL5, DMP1, IFIH1, IFITM1, IRF7, IRF9, LAMP3, OAS1, OAS2, OAS3 and STAT1. Transcriptional activity is independent of kinase activity.


亚细胞位置:
Cytoplasm Cytoskeleton Spindle Nucleus Cytoplasm Cytoskeleton Microtubule organizing center Centrosome Cytoplasm Associated with the spindle during prometaphase and metaphase (By similarity). PEA15-binding and phosphorylated DAPK1 promote its cytoplasmic retention. Phosphorylation at Ser- 246 and Ser-248 as well as autophosphorylation at Thr-190 promote nuclear localization.


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