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首页  >  酶联免疫试剂盒  >  Human MIF ELISA Kit
MIF (基因名), Macrophage migration inhibitory factor (蛋白名), MIF_HUMAN.
产品名称:

Human MIF/ Macrophage migration inhibitory factor ELISA Kit
巨噬细胞迁移抑制因子

货号:

E0698h

商标:
EIAab®
监管等级:
别名:

MIF, Glycosylation-inhibiting factor, GIF, L-dopachrome isomerase, L-dopachrome tautomerase, Phenylpyruvate tautomerase, GLIF, MMIF

检测方法:
ELISA
实验类型:
Sandwich
检测范围:
0.312-20ng/mL
灵敏度:
0.02ng/mL
特异性:
Natural and recombinant human Macrophage migration inhibitory factor
样品类型:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
样品数据:
实验步骤:
实验步骤
研究领域:
Immunology
Human MIF ELISA Kit
规格 & 价格: cart
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Human MIF ELISA Kit
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产品说明书
说明书: 下载说明书
MSDS: MSDS


精密度
批内差:已知浓度的3个样本在一个板子内重复检测20次,以评估批内精密度。
批内 CV: ≤3.8%
批间差:已知浓度的3个样本在不同的板子上重复测定5次,以评估测定批间精密度。
批间 CV: ≤7.5%
回收率
回收率:低、中和高浓度的分析物被掺入到血清或者血浆样本中,进行回收实验测定。

Sample Type

Average(%)

Recovery Range(%)

Serum

87

81-93

Plasma

89

83-95

 

 

 

 

线性
线性:给定样本通过梯度稀释,每次稀释的测量值与理论值的比值。

Sample

1:2

1:4

1:8

1:16

serum(n=5)

107-116%

91-101%

95-104%

115-124%

EDTA plasma(n=5)

90-99%

113-122%

93-102%

88-96%

heparin plasma(n=5)

97-108%

 

98-107%

87-95%

111-120%

 

通用注释


亚单元:
Homotrimer. Interacts with CXCR2 extracellular domain (By similarity). Interacts with the CD74 extracellular domain, COPS5 and BNIPL.


功能:
Pro-inflammatory cytokine. Involved in the innate immune response to bacterial pathogens. The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense. Counteracts the anti-inflammatory activity of glucocorticoids. Has phenylpyruvate tautomerase and dopachrome tautomerase activity (in vitro), but the physiological substrate is not known. It is not clear whether the tautomerase activity has any physiological relevance, and whether it is important for cytokine activity.


亚细胞位置:
Secreted Cytoplasm Does not have a cleavable signal sequence and is secreted via a specialized, non-classical pathway. Secreted by macrophages upon stimulation by bacterial lipopolysaccharide (LPS), or by M.tuberculosis antigens.


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