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MICA (GeneName), MHC class I polypeptide-related sequence A (ProteinName), MICA_HUMAN.
Product Name:

Human MICA/ MHC class I polypeptide-related sequence A ELISA Kit

Cat.#:

E1845h

Brand:
EIAab®
Regulatory Status:
Alternative:

MIC-A, PERB11.1

Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
78-5000pg/mL
Sensitivity:
33pg/mL
Specificity:
Natural and recombinant human MHC class I polypeptide-related sequence A
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Assay Procedure
Research Area:
Immunology
Human MICA ELISA Kit
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Human MICA ELISA Kit
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Product Datasheets
Instruction: Down Instruction
MSDS: MSDS


Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤5.6%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤8.9%

Recovery
Recovery was determined by spiking various levels of MHC class I polypeptide-related sequence A into serum and plasma.

Sample Type

Average(%)

Recovery Range(%)

Serum

107

101-113

Plasma

109

103-115

 

 

 

 

Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of MHC class I polypeptide-related sequence A and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

91-101%

85-94%

90-99%

104-114%

EDTA plasma(n=5)

83-94%

103-115%

82-92%

80-93%

heparin plasma(n=5)

107-117%

 

93-105%

85-95%

111-120%

 

General Annotation


Sub Unit:
Unlike classical MHC class I molecules, does not form a heterodimer with beta-2-microglobulin. Binds as a monomer to a KLRK1/NKG2D homodimer. KLRK1 forms a complex with HCST/DAP10 in which KLRK1 binds MICA while HCST acts as an adapter molecule which enables signal transduction. Interacts with PDIA6 on the surface of tumor cells, leading to disulfide bond reduction which is required for release of MICA from tumor cells. Interacts with human cytomegalovirus/HHV-5 protein UL142.


Function:
Seems to have no role in antigen presentation. Acts as a stress-induced self-antigen that is recognized by gamma delta T-cells. Ligand for the KLRK1/NKG2D receptor. Binding to KLRK1 leads to cell lysis.


Subcellular Location:
Cell membrane Single-pass type I membrane protein Cytoplasm Expressed on the cell surface in gastric epithelium, endothelial cells and fibroblasts and in the cytoplasm in keratinocytes and monocytes. Infection with human adenovirus 5 suppresses cell surface expression due to the adenoviral E3-19K protein which causes retention in the endoplasmic reticulum.



[1].
"The MICA-A9 triplet repeat polymorphism in the transmembrane region confers additional susceptibility to the development of psoriatic arthritis and is independent of the association of Cw*0602 in psoriasis."

[2].
"A second lineage of mammalian major histocompatibility complex class I genes."

[3].
"Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study."

[4].
"Examination of genetic polymorphisms in newborns for signatures of sex-specific prenatal selection."

[5].
"[Association of MICA gene polymorphism and serum soluble MICA level with colorectal cancer]."

[6].
"Association analysis of MICA gene polymorphism and MICA-129 dimorphism with inflammatory bowel disease susceptibility in a Spanish population."

[7].
"MICA polymorphisms and haplotypes with HLA-B and HLA-DRB1 in Koreans."

[8].
"MICA polymorphism in a northern Chinese Han population: The identification of a new MICA allele, MICA*059."

[9].
"An influence of HLA-A, B, DR, DQ, and MICA on the occurrence of Celiac disease in patients with type 1 diabetes."

[10].
"The major genetic determinants of HIV-1 control affect HLA class I peptide presentation."
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