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首页  >  酶联免疫试剂盒  >  Mouse Mia2 ELISA Kit
Mia2 (基因名), Melanoma inhibitory activity protein 2 (蛋白名), MIA2_MOUSE.
产品名称:

Mouse Mia2/ Melanoma inhibitory activity protein 2 ELISA Kit
黑色素瘤活性抑制蛋白2

货号:

E13472m

商标:
EIAab®
监管等级:
别名:

CTAGE family member 5 ER export factor, Ctage5

检测方法:
ELISA
实验类型:
Competitive
检测范围:
0.312-20ng/mL
灵敏度:
0.178ng/mL
特异性:
Natural and recombinant mouse Melanoma inhibitory activity protein 2
样品类型:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
样品数据:
实验步骤:
实验步骤
研究领域:
Cancer
Mouse Mia2 ELISA Kit
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Mouse Mia2 ELISA Kit
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产品说明书
说明书: 下载说明书
MSDS: MSDS


精密度
批内差:已知浓度的3个样本在一个板子内重复检测20次,以评估批内精密度。
批内 CV: ≤8.3%
批间差:已知浓度的3个样本在不同的板子上重复测定5次,以评估测定批间精密度。
批间 CV: ≤10.2%
回收率
回收率:低、中和高浓度的分析物被掺入到血清或者血浆样本中,进行回收实验测定。

Sample Type

Average(%)

Recovery Range(%)

Serum

90

84-96

Plasma

92

86-98

 

 

 

 

线性
线性:给定样本通过梯度稀释,每次稀释的测量值与理论值的比值。

Sample

1:2

1:4

1:8

1:16

serum(n=5)

80-93%

108-118%

103-115%

103-112%

EDTA plasma(n=5)

82-93%

99-108%

89-99%

91-101%

heparin plasma(n=5)

94-104%

 

105-117%

103-112%

95-104%

 

通用注释


亚单元:
Interacts with MIA3 (By similarity). Interacts with the COPII coat subunits SEC23A, SEC23B and maybe SEC24C (By similarity) (PubMed:21807889). Interacts with PREB; recruits PREB to endoplasmic reticulum exit sites. Interacts with APOB (By similarity).


功能:
Plays a role in the transport of cargos that are too large to fit into COPII-coated vesicles and require specific mechanisms to be incorporated into membrane-bound carriers and exported from the endoplasmic reticulum. Plays a role in the secretion of lipoproteins, pre-chylomicrons and pre-VLDLs, by participating in their export from the endoplasmic reticulum (By similarity). Thereby, may play a role in cholesterol and triglyceride homeostasis (PubMed:21807889). Required for collagen VII (COL7A1) secretion by loading COL7A1 into transport carriers and recruiting PREB/SEC12 at the endoplasmic reticulum exit sites (By similarity).


亚细胞位置:
Endoplasmic reticulum membrane Single-pass membrane protein Localizes to endoplasmic reticulum exit sites (ERES), also known as transitional endoplasmic reticulum (tER).


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