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MIA2 (GeneName), Melanoma inhibitory activity protein 2 (ProteinName), MIA2_HUMAN.
Product Name:

Human MIA2/ Melanoma inhibitory activity protein 2 ELISA Kit

Cat.#:

E13472h

Brand:
EIAab®
Regulatory Status:
Alternative:

CTAGE family member 5 ER export factor, Meningioma-expressed antigen 6/11, CTAGE5, MEA11, MEA6, MGEA11, MGEA6

Detection Method:
ELISA
Assay Type:
Competitive
Detection Range:
0.312-20ng/mL
Sensitivity:
0.176ng/mL
Specificity:
Natural and recombinant human Melanoma inhibitory activity protein 2
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Assay Procedure
Research Area:
Cancer
Human MIA2 ELISA Kit
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Product Datasheets
Instruction: Down Instruction
MSDS: MSDS


Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤7.3%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤10.7%

Recovery
Recovery was determined by spiking various levels of Melanoma inhibitory activity protein 2 into serum and plasma.

Sample Type

Average(%)

Recovery Range(%)

Serum

94

88-100

Plasma

96

90-102

 

 

 

 

Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Melanoma inhibitory activity protein 2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

88-99%

107-117%

111-120%

106-115%

EDTA plasma(n=5)

99-109%

88-101%

102-112%

95-107%

heparin plasma(n=5)

101-112%

 

100-109%

107-107%

99-109%

 

General Annotation


Sub Unit:
Interacts with MIA3 (PubMed:21525241, PubMed:25202031, PubMed:27138255, PubMed:27170179). Interacts with the COPII coat subunits SEC23A, SEC23B and maybe SEC24C (PubMed:21525241, PubMed:27551091). Interacts with PREB; recruits PREB to endoplasmic reticulum exit sites (PubMed:25202031, PubMed:27170179). Interacts with APOB (PubMed:27138255).


Function:
Plays a role in the transport of cargos that are too large to fit into COPII-coated vesicles and require specific mechanisms to be incorporated into membrane-bound carriers and exported from the endoplasmic reticulum (PubMed:27138255, PubMed:21525241, PubMed:25202031, PubMed:27170179). Plays a role in the secretion of lipoproteins, pre-chylomicrons and pre-VLDLs, by participating in their export from the endoplasmic reticulum (PubMed:27138255). Thereby, may play a role in cholesterol and triglyceride homeostasis (By similarity). Required for collagen VII (COL7A1) secretion by loading COL7A1 into transport carriers and recruiting PREB/SEC12 at the endoplasmic reticulum exit sites (PubMed:21525241, PubMed:25202031, PubMed:27170179).


Subcellular Location:
Endoplasmic reticulum membrane Single-pass membrane protein Localizes to endoplasmic reticulum exit sites (ERES), also known as transitional endoplasmic reticulum (tER).


This product has not yet been referenced specifically in any publications.

[1].
"TANGO1 and Mia2/cTAGE5 (TALI) cooperate to export bulky pre-chylomicrons/VLDLs from the endoplasmic reticulum."

[2].
"Specific expression and regulation of the new melanoma inhibitory activity-related gene MIA2 in hepatocytes."

[3].
"An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."

[4].
"Reduced cholesterol and triglycerides in mice with a mutation in Mia2, a liver protein that localizes to ER exit sites."

[5].
"The consensus coding sequences of human breast and colorectal cancers."

[6].
"Complete sequencing and characterization of 21,243 full-length human cDNAs."

[7].
"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."

[8].
"The DNA sequence and analysis of human chromosome 14."

[9].
"MGEA6 is tumor-specific overexpressed and frequently recognized by patient-serum antibodies."

[10].
"Architecture of the human interactome defines protein communities and disease networks."
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