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Mgp (GeneName), Matrix Gla protein (ProteinName), MGP_MOUSE.
Product Name:

Mouse Mgp/ Matrix Gla protein ELISA Kit

Cat.#:

E1477m

Brand:
EIAab®
Regulatory Status:
Alternative:

MGP, Mglap

Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
78-5000pg/mL
Sensitivity:
39.22pg/mL
Specificity:
Natural and recombinant mouse Matrix Gla protein
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Assay Procedure
Research Area:
-
Mouse Mgp ELISA Kit
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Product Datasheets
Instruction: Down Instruction
MSDS: MSDS


Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤6.9%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤8.8%

Recovery
Recovery was determined by spiking various levels of Matrix Gla protein into serum and plasma.

Sample Type

Average(%)

Recovery Range(%)

Serum

88

82-94

Plasma

90

84-96

 

 

 

 

Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Matrix Gla protein and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

85-94%

104-113%

81-91%

101-109%

EDTA plasma(n=5)

107-119%

80-91%

87-95%

107-117%

heparin plasma(n=5)

99-109%

 

83-93%

95-105%

106-116%

 

General Annotation


Sub Unit:
N/A


Function:
Associates with the organic matrix of bone and cartilage. Thought to act as an inhibitor of bone formation.


Subcellular Location:
Secreted


This product has not yet been referenced specifically in any publications.

[1].
"Molecular structure, chromosome assignment, and promoter organization of the human matrix Gla protein gene."

[2].
"Polymorphisms in MGP gene and their association with lead toxicity."

[3].
"Individual susceptibility and genotoxicity in workers exposed to hazardous materials like lead."

[4].
"The local calcification inhibitor matrix Gla protein in pseudoxanthoma elasticum."

[5].
"A polymorphism of matrix Gla protein gene is associated with kidney stones."

[6].
"Matrix Gla protein and osteopontin genetic associations with coronary artery calcification and bone density: the CARDIA study."

[7].
"Matrix GLA protein gene polymorphisms: clinical correlates and cardiovascular mortality in chronic kidney disease patients."

[8].
"T-138C polymorphism of matrix gla protein promoter alters its expression but is not directly associated with atherosclerotic vascular calcification."

[9].
"Association of natural tooth loss with genetic variation at the human matrix Gla protein locus in elderly women."

[10].
"[Study on association of polymorphism of MGP(T-138C) gene and blood lead level in children]."
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