027-59234612(+86)
EIAab
Home  >  ELISA Kit  >  Human MGMT ELISA Kit
MGMT (GeneName), Methylated-DNA--protein-cysteine methyltransferase (ProteinName), MGMT_HUMAN.
Product Name:

Human MGMT/ Methylated-DNA--protein-cysteine methyltransferase ELISA Kit

Cat.#:

E2027h

Brand:
EIAab®
Regulatory Status:
Alternative:

6-O-methylguanine-DNA methyltransferase, MGMT, O-6-methylguanine-DNA-alkyltransferase

Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
0.156-10ng/mL
Sensitivity:
0.049ng/mL
Specificity:
Natural and recombinant human Methylated-DNA--protein-cysteine methyltransferase
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Assay Procedure
Research Area:
Cancer
Human MGMT ELISA Kit
Size & Price: Sign In.
×
Human MGMT ELISA Kit
Email *
Message *
Please Sign In.
Product Datasheets
Datasheet: Down Datasheet
Instruction: Down Instruction
MSDS: MSDS


Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤6.5%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤10.2%

Recovery
Recovery was determined by spiking various levels of Methylated-DNA--protein-cysteine methyltransferase into serum and plasma.

Sample Type

Average(%)

Recovery Range(%)

Serum

102

96-108

Plasma

104

98-110

 

 

 

 

Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Methylated-DNA--protein-cysteine methyltransferase and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

88-97%

103-113%

95-105%

100-110%

EDTA plasma(n=5)

81-92%

99-108%

98-108%

114-124%

heparin plasma(n=5)

109-119%

 

106-118%

113-125%

87-97%

 

General Annotation


Sub Unit:
N/A


Function:
Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.


Subcellular Location:
Nucleus


This product has not yet been referenced specifically in any publications.

[1].
"Polymorphisms of the DNA repair gene MGMT and risk and progression of head and neck cancer."

[2].
"MGMT methylation is associated primarily with the germline C>T SNP (rs16906252) in colorectal cancer and normal colonic mucosa."

[3].
"Effect of DNA repair host factors on temozolomide or dacarbazine melanoma treatment in Caucasians."

[4].
"Association between MGMT promoter hypermethylation and p53 mutation in glioblastoma."

[5].
"High incidence of MGMT promoter methylation in primary glioblastomas without correlation with TP53 gene mutations."
Close
Sample Data
Close
Sample Data
Close
Sample Data
My Cart close
Attention close
History close
Clear All
Comparison close
User
My Cart
Attention
History
Comparison
Top
Message
close_message
Product Name *
Email *
Message: *
Inform
new Message
Size Quantity Price ($) Subtotal 1 ($)
Subtotal 2: $
triangle
Size Quantity Price ($)
Do you want to be our agent and get a lower discount?
Please contact us:
Tel:027-59234612(+86)
Fax:027-59759960(+86)
Email:sales@eiaab.com