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MGMT (GeneName), Methylated-DNA--protein-cysteine methyltransferase (ProteinName), MGMT_CANFA.
Product Name:

Dog MGMT/ Methylated-DNA--protein-cysteine methyltransferase ELISA Kit

Cat.#:

E2027d

Brand:
EIAab®
Regulatory Status:
Alternative:

6-O-methylguanine-DNA methyltransferase, MGMT, O-6-methylguanine-DNA-alkyltransferase

Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
78-5000pg/mL
Sensitivity:
18pg/mL
Specificity:
Natural and recombinant dog Methylated-DNA--protein-cysteine methyltransferase
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Assay Procedure
Research Area:
-
Dog MGMT ELISA Kit
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Product Datasheets
Instruction: Down Instruction
MSDS: MSDS


Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Methylated-DNA--protein-cysteine methyltransferase and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

99-109%

99-109%

88-100%

98-107%

EDTA plasma(n=5)

83-95%

107-115%

109-119%

84-84%

heparin plasma(n=5)

105-115%

 

97-107%

109-119%

110-119%

 

General Annotation


Sub Unit:
N/A


Function:
Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.


Subcellular Location:
Nucleus


This product has not yet been referenced specifically in any publications.

[1].
"Polymorphisms of the DNA repair gene MGMT and risk and progression of head and neck cancer."

[2].
"MGMT methylation is associated primarily with the germline C>T SNP (rs16906252) in colorectal cancer and normal colonic mucosa."

[3].
"Effect of DNA repair host factors on temozolomide or dacarbazine melanoma treatment in Caucasians."

[4].
"Association between MGMT promoter hypermethylation and p53 mutation in glioblastoma."

[5].
"High incidence of MGMT promoter methylation in primary glioblastomas without correlation with TP53 gene mutations."
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