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Mgme1 (GeneName), Mitochondrial genome maintenance exonuclease 1 (ProteinName), MGME1_MOUSE.
Product Name:

Mouse Mgme1/ Mitochondrial genome maintenance exonuclease 1 ELISA Kit

Cat.#:

E15155m

Brand:
EIAab®
Regulatory Status:
Detection Method:
ELISA
Assay Type:
Sandwich
Specificity:
Natural and recombinant mouse Mitochondrial genome maintenance exonuclease 1
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Assay Procedure
Research Area:
-
Mouse Mgme1 ELISA Kit
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Mouse Mgme1 ELISA Kit
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Product Datasheets
Instruction: Down Instruction
MSDS: MSDS


Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: 0

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: 0

Recovery
Recovery was determined by spiking various levels of Mitochondrial genome maintenance exonuclease 1 into serum and plasma.

Sample Type

Average(%)

Recovery Range(%)

Serum

80

80-80

Plasma

80

80-80

 

 

 

 

Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Mitochondrial genome maintenance exonuclease 1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

88-98%

102-113%

90-100%

95-104%

EDTA plasma(n=5)

81-91%

91-100%

88-101%

85-95%

heparin plasma(n=5)

95-106%

 

100-110%

109-119%

109-120%

 

General Annotation


Sub Unit:
N/A


Function:
Metal-dependent single-stranded DNA (ssDNA) exonuclease involved in mitochondrial genome maintenance. Has preference for 5'-3' exonuclease activity but is also capable of endoduclease activity on linear substrates. Necessary for maintenance of proper 7S DNA levels. Probably involved in mitochondrial DNA (mtDNA) repair, possibly via the processing of displaced DNA containing Okazaki fragments during RNA-primed DNA synthesis on the lagging strand or via processing of DNA flaps during long-patch base excision repair (By similarity). Specifically binds 5-hydroxymethylcytosine (5hmC)-containing DNA in stem cells.


Subcellular Location:
Mitochondrion


This product has not yet been referenced specifically in any publications.

[1].
"Loss-of-function mutations in MGME1 impair mtDNA replication and cause multisystemic mitochondrial disease."

[2].
"Identification of a novel human mitochondrial endo-/exonuclease Ddk1/c20orf72 necessary for maintenance of proper 7S DNA levels."

[3].
"Dynamic readers for 5-(hydroxy)methylcytosine and its oxidized derivatives."

[4].
"Complete sequencing and characterization of 21,243 full-length human cDNAs."

[5].
"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."

[6].
"The DNA sequence and comparative analysis of human chromosome 20."

[7].
"Mass spectrometry analyses of normal and polyglutamine expanded ataxin-3 reveal novel interaction partners involved in mitochondrial function."

[8].
"Architecture of the human interactome defines protein communities and disease networks."

[9].
"Homozygous c.359del variant in MGME1 is associated with early onset cerebellar ataxia."

[10].
"A Map of Human Mitochondrial Protein Interactions Linked to Neurodegeneration Reveals New Mechanisms of Redox Homeostasis and NF-κB Signaling."
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