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MGAM (GeneName), Maltase-glucoamylase, intestinal (ProteinName), MGA_HUMAN.
Product Name:

Human MGAM/ Maltase-glucoamylase, intestinal ELISA Kit

Cat.#:

E0253h

Brand:
EIAab®
Regulatory Status:
Alternative:

MGA, MGAML

Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
1.56-100U/L
Sensitivity:
0.78U/L
Specificity:
Natural and recombinant human Maltase-glucoamylase, intestinal
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Assay Procedure
Research Area:
-
Human MGAM ELISA Kit
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Human MGAM ELISA Kit
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Product Datasheets
Datasheet: Down Datasheet
Instruction: Down Instruction
MSDS: MSDS


Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤4.5%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.3%

Recovery
Recovery was determined by spiking various levels of Maltase-glucoamylase, intestinal into serum and plasma.

Sample Type

Average(%)

Recovery Range(%)

Serum

94

88-100

Plasma

96

90-102

 

 

 

 

Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Maltase-glucoamylase, intestinal and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

106-115%

97-108%

90-99%

108-118%

EDTA plasma(n=5)

110-120%

91-101%

95-104%

107-117%

heparin plasma(n=5)

102-111%

 

81-92%

97-108%

103-113%

 

General Annotation


Sub Unit:
Monomer.


Function:
May serve as an alternate pathway for starch digestion when luminal alpha-amylase activity is reduced because of immaturity or malnutrition. May play a unique role in the digestion of malted dietary oligosaccharides used in food manufacturing.


Subcellular Location:
Apical cell membrane Single-pass type II membrane protein Brush border.


This product has not yet been referenced specifically in any publications.

[1].
"Human small intestinal maltase-glucoamylase cDNA cloning. Homology to sucrase-isomaltase."

[2].
"Human intestinal maltase-glucoamylase: crystal structure of the N-terminal catalytic subunit and basis of inhibition and substrate specificity."

[3].
"Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study."

[4].
"Structural basis for substrate selectivity in human maltase-glucoamylase and sucrase-isomaltase N-terminal domains."

[5].
"A new diagnostic assay for glycogen storage disease type II in mixed leukocytes."

[6].
"Comparison of maltose and acarbose as inhibitors of maltase-glucoamylase activity in assaying acid alpha-glucosidase activity in dried blood spots for the diagnosis of infantile Pompe disease."

[7].
"Direct multiplex assay of enzymes in dried blood spots by tandem mass spectrometry for the newborn screening of lysosomal storage disorders."

[8].
"Inhibition of recombinant human maltase glucoamylase by salacinol and derivatives."

[9].
"The DNA sequence of human chromosome 7."

[10].
"The maltase-glucoamylase gene: common ancestry to sucrase-isomaltase with complementary starch digestion activities."
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