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MFSD2A (GeneName), Major facilitator superfamily domain-containing protein 2A (ProteinName), MFS2A_HUMAN.
Product Name:

Human MFSD2A/ Major facilitator superfamily domain-containing protein 2A ELISA Kit

Cat.#:

E15095h

Brand:
EIAab®
Regulatory Status:
Alternative:

MFSD2, HMFN0656, PP9177, UNQ300/PRO341

Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
0.156-10ng/ml
Sensitivity:
0.078ng/ml
Specificity:
Natural and recombinant human Major facilitator superfamily domain-containing protein 2A
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Assay Procedure
Research Area:
-
Human MFSD2A ELISA Kit
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Human MFSD2A ELISA Kit
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Product Datasheets
Datasheet: Down Datasheet
Instruction: Down Instruction
MSDS: MSDS


Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤4.6%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤6.6%

Recovery
Recovery was determined by spiking various levels of Major facilitator superfamily domain-containing protein 2A into serum and plasma.

Sample Type

Average(%)

Recovery Range(%)

Serum

87

81-93

Plasma

89

83-95

 

 

 

 

Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Major facilitator superfamily domain-containing protein 2A and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

96-106%

98-108%

117-127%

94-105%

EDTA plasma(n=5)

92-104%

110-120%

86-98%

117-126%

heparin plasma(n=5)

89-99%

 

85-94%

105-116%

98-110%

 

General Annotation


Sub Unit:
N/A


Function:
Play a role in thermogenesis via beta-adrenergic signaling pathway.


Subcellular Location:
Endoplasmic reticulum membrane Multi-pass membrane protein


This product has not yet been referenced specifically in any publications.

[1].
"Mfsd2a encodes a novel major facilitator superfamily domain-containing protein highly induced in brown adipose tissue during fasting and adaptive thermogenesis."

[2].
"Structural Insights into the Transport Mechanism of the Human Sodium-dependent Lysophosphatidylcholine Transporter MFSD2A."

[3].
"A partially inactivating mutation in the sodium-dependent lysophosphatidylcholine transporter MFSD2A causes a non-lethal microcephaly syndrome."

[4].
"Inactivating mutations in MFSD2A, required for omega-3 fatty acid transport in brain, cause a lethal microcephaly syndrome."

[5].
"Mfsd2a is critical for the formation and function of the blood-brain barrier."

[6].
"Mfsd2a is a transporter for the essential omega-3 fatty acid docosahexaenoic acid."

[7].
"MFSD2a, the Syncytin-2 receptor, is important for trophoblast fusion."

[8].
"A haploid genetic screen identifies the major facilitator domain containing 2A (MFSD2A) transporter as a key mediator in the response to tunicamycin."

[9].
"A placenta-specific receptor for the fusogenic, endogenous retrovirus-derived, human syncytin-2."

[10].
"The DNA sequence and biological annotation of human chromosome 1."
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