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Home  >  ELISA Kit  >  Rat Mfge8 ELISA Kit
Mfge8 (GeneName), Lactadherin (ProteinName), MFGM_RAT.
Product Name:

Rat Mfge8/ Lactadherin ELISA Kit

Cat.#:

E2304r

Brand:
EIAab®
Regulatory Status:
Alternative:

MFGM, Milk fat globule-EGF factor 8, MFG-E8, O-acetyl GD3 ganglioside synthase, AGS, SED1, Ags

Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
31.2-2000pg/mL
Sensitivity:
15.61pg/mL
Specificity:
Natural and recombinant rat Lactadherin
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Assay Procedure
Research Area:
-
Rat Mfge8 ELISA Kit
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Rat Mfge8 ELISA Kit
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Product Datasheets
Datasheet: Down Datasheet
Instruction: Down Instruction
MSDS: MSDS


Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤5.2%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤9.1%

Recovery
Recovery was determined by spiking various levels of Lactadherin into serum and plasma.

Sample Type

Average(%)

Recovery Range(%)

Serum

100

94-106

Plasma

102

96-108

 

 

 

 

Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Lactadherin and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

81-81%

93-104%

85-94%

92-102%

EDTA plasma(n=5)

88-98%

90-100%

105-115%

87-96%

heparin plasma(n=5)

109-119%

 

110-120%

107-116%

105-113%

 

General Annotation


Sub Unit:
N/A


Function:
Contributes to phagocytic removal of apoptotic cells in many tissues. Plays an important role in the maintenance of intestinal epithelial homeostasis and the promotion of mucosal healing. Promotes VEGF-dependent neovascularization (By similarity). Specific ligand for the alpha-v/beta-3 and alpha-v/beta-5 receptors. Also binds to phosphatidylserine-enriched cell surfaces in a receptor-independent manner. Zona pellucida-binding protein which may play a role in gamete interaction. Appears to participate in the O-acetylation of GD3 ganglioside sialic acid.


Subcellular Location:
Membrane Peripheral membrane protein Secreted


This product has not yet been referenced specifically in any publications.

[1].
"SED1/MFG-E8: a bi-motif protein that orchestrates diverse cellular interactions."

[2].
"Genetic polymorphism in milk fat globule-EGF factor 8 (MFG-E8) is associated with systemic lupus erythematosus in human."

[3].
"Medin: an integral fragment of aortic smooth muscle cell-produced lactadherin forms the most common human amyloid."

[4].
"Mapping of a newly discovered human gene homologous to the apoptosis associated-murine mammary protein, MFG-E8, to chromosome 15q25."

[5].
"Lactadherin (formerly BA46), a membrane-associated glycoprotein expressed in human milk and breast carcinomas, promotes Arg-Gly-Asp (RGD)-dependent cell adhesion."

[6].
"Cloning and sequence analysis of human breast epithelial antigen BA46 reveals an RGD cell adhesion sequence presented on an epidermal growth factor-like domain."

[7].
"Sequential use of transcriptional profiling, expression quantitative trait mapping, and gene association implicates MMP20 in human kidney aging."

[8].
"Cross-beta spine architecture of fibrils formed by the amyloidogenic segment NFGSVQFV of medin from solid-state NMR and X-ray fiber diffraction measurements."

[9].
"p63(TP63) elicits strong trans-activation of the MFG-E8/lactadherin/BA46 gene through interactions between the TA and DeltaN isoforms."

[10].
"Role of aggregated medin in the pathogenesis of thoracic aortic aneurysm and dissection."
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