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MFAP5 (GeneName), Microfibrillar-associated protein 5 (ProteinName), MFAP5_HUMAN.
Product Name:

Human MFAP5/ Microfibrillar-associated protein 5 ELISA Kit

Cat.#:

E11955h

Brand:
EIAab®
Regulatory Status:
Alternative:

MFAP-5, MP25, Microfibril-associated glycoprotein 2, MAGP-2, MAGP2

Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
0.312-20ng/mL
Sensitivity:
0.15ng/ml
Specificity:
Natural and recombinant human Microfibrillar-associated protein 5
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Assay Procedure
Research Area:
-
Human MFAP5 ELISA Kit
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Human MFAP5 ELISA Kit
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Product Datasheets
Datasheet: Down Datasheet
Instruction: Down Instruction
MSDS: MSDS


Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤4.6%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤8.3%

Recovery
Recovery was determined by spiking various levels of Microfibrillar-associated protein 5 into serum and plasma.

Sample Type

Average(%)

Recovery Range(%)

Serum

89

83-95

Plasma

91

85-97

 

 

 

 

Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Microfibrillar-associated protein 5 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

106-116%

82-92%

105-117%

104-116%

EDTA plasma(n=5)

109-119%

98-107%

99-109%

101-113%

heparin plasma(n=5)

99-111%

 

100-110%

96-105%

94-107%

 

General Annotation


Sub Unit:
Interacts with TGFB2. Interacts with BMP2. Interacts with FBN1 (via N-terminal domain) and FBN2.


Function:
May play a role in hematopoiesis. In the cardiovascular system, could regulate growth factors or participate in cell signaling in maintaining large vessel integrity (By similarity). Component of the elastin-associated microfibrils (PubMed:8557636).


Subcellular Location:
Secreted Extracellular space Extracellular matrix


This product has not yet been referenced specifically in any publications.

[1].
"The exon structure of the human MAGP-2 gene. Similarity with the MAGP-1 gene is confined to two exons encoding a cysteine-rich region."

[2].
"Further characterization of proteins associated with elastic fiber microfibrils including the molecular cloning of MAGP-2 (MP25)"

[3].
"MFAP5 loss-of-function mutations underscore the involvement of matrix alteration in the pathogenesis of familial thoracic aortic aneurysms and dissections."

[4].
"A gene signature predictive for outcome in advanced ovarian cancer identifies a survival factor: microfibril-associated glycoprotein 2."

[5].
"Microfibril-associate glycoprotein-2 (MAGP-2) promotes angiogenic cell sprouting by blocking notch signaling in endothelial cells."

[6].
"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."

[7].
"MFAP5 promotes tumor progression and bone metastasis by regulating ERK/MMP signaling pathways in breast cancer."

[8].
"MFAP5 and TNNC1: Potential markers for predicting occult cervical lymphatic metastasis and prognosis in early stage tongue cancer."

[9].
"Characterization of the Extracellular Matrix of Normal and Diseased Tissues Using Proteomics."

[10].
"MAGP2 controls Notch via interactions with RGD binding integrins: Identification of a novel ECM-integrin-Notch signaling axis."
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