本公司产品仅供科研使用,不用于人体及临床诊断。
027-59234612(+86)
我的积分
注册  /  登录
EIAab
首页  >  酶联免疫试剂盒  >  Mouse Met ELISA Kit
Met (基因名), Hepatocyte growth factor receptor (蛋白名), MET_MOUSE.
产品名称:

Mouse Met/ Hepatocyte growth factor receptor ELISA Kit
肝细胞生长因子受体

货号:

E9559m

商标:
EIAab®
监管等级:
别名:

HGF receptor, HGF/SF receptor, Proto-oncogene c-Met, Scatter factor receptor, SF receptor, Tyrosine-protein kinase Met

检测方法:
ELISA
实验类型:
Sandwich
检测范围:
31.2-2000pg/mL
灵敏度:
15.61pg/mL
特异性:
Natural and recombinant mouse Hepatocyte growth factor receptor
样品类型:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
样品数据:
实验步骤:
实验步骤
研究领域:
-
Mouse Met ELISA Kit
规格 & 价格: cart
×
Mouse Met ELISA Kit
邮箱 *
消息 *
Please 登录.
产品说明书
说明书: 下载说明书
MSDS: MSDS


精密度
批内差:已知浓度的3个样本在一个板子内重复检测20次,以评估批内精密度。
批内 CV: ≤6.4%
批间差:已知浓度的3个样本在不同的板子上重复测定5次,以评估测定批间精密度。
批间 CV: ≤8.2%
回收率
回收率:低、中和高浓度的分析物被掺入到血清或者血浆样本中,进行回收实验测定。

Sample Type

Average(%)

Recovery Range(%)

Serum

88

82-94

Plasma

90

84-96

 

 

 

 

线性
线性:给定样本通过梯度稀释,每次稀释的测量值与理论值的比值。

Sample

1:2

1:4

1:8

1:16

serum(n=5)

99-108%

111-121%

99-112%

105-117%

EDTA plasma(n=5)

89-100%

85-97%

93-103%

96-106%

heparin plasma(n=5)

100-109%

 

84-94%

86-96%

109-119%

 

通用注释


亚单元:
Heterodimer made of an alpha chain (50 kDa) and a beta chain (145 kDa) which are disulfide linked (By similarity). Binds PLXNB1 (By similarity). Interacts when phosphorylated with downstream effectors including STAT3, PIK3R1, SRC, PCLG1, GRB2 and GAB1 (By similarity). When phosphorylated at Tyr-1354, interacts with INPPL1/SHIP2 (By similarity). Interacts with RANBP9 and RANBP10 (By similarity). Interacts with INPP5D/SHIP1 (PubMed:11896575). Interacts with SPSB1, SPSB2, SPSB4 and probably SPSB3. SPSB1 binding occurs in the presence and in the absence of HGF, however HGF treatment has a positive effect on this interaction (PubMed:16369487). Interacts with MUC20; prevents interaction with GRB2 and suppresses hepatocyte growth factor-induced cell proliferation (PubMed:15314156). Interacts with GRB10 (By similarity). Interacts with PTPN1 and PTPN2. Interacts with HSP90AA1 and HSP90AB1; the interaction suppresses MET kinase activity.


功能:
Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to hepatocyte growth factor/HGF ligand. Regulates many physiological processes including proliferation, scattering, morphogenesis and survival. Ligand binding at the cell surface induces autophosphorylation of MET on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, interacts with the PI3-kinase subunit PIK3R1, PLCG1, SRC, GRB2, STAT3 or the adapter GAB1. Recruitment of these downstream effectors by MET leads to the activation of several signaling cascades including the RAS-ERK, PI3 kinase-AKT, or PLCgamma-PKC. The RAS-ERK activation is associated with the morphogenetic effects while PI3K/AKT coordinates prosurvival effects. During embryonic development, MET signaling plays a role in gastrulation, development and migration of muscles and neuronal precursors, angiogenesis and kidney formation. In adults, participates in wound healing as well as organ regeneration and tissue remodeling. Promotes also differentiation and proliferation of hematopoietic cells (By similarity). May regulate cortical bone osteogenesis (PubMed:26637977).


亚细胞位置:
Membrane Single-pass type I membrane protein


该产品尚未在任何出版物中被引用。
关闭
Sample Data
关闭
Sample Data
关闭
Sample Data
用户中心 close
购物车 close
我的收藏 close
我的足迹 close
清除
产品对比 close
用户中心
购物车
我的收藏
我的足迹
产品对比
回到顶部
消息
close_message
产品名称 *
邮箱 *
消息 *
通知
new 留 言
规格 数量 单价 (¥) 小计 1 (¥)
小计 2:
triangle
规格 数量 单价 (¥)
你想做我们的代理并得到更低的折扣吗?
请联系我们:
电话:027-59234612(+86)
传真:027-59234610(+86)
邮箱:sales@eiaab.com